Gut microbiota and functional metabolic predictions in white feces disease-infected Pacific white shrimp, Penaeus vannamei, from Indonesian farms
The etiological agent of white feces disease (WFD) infecting Pacific white shrimp Penaeus vannamei in Indonesia farms remains obscure. The present study aimed to identify possible causative agents of WFD infection in Pacific white shrimps cultured in Indonesian farms. WFD-infected and healthy sample...
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| Format: | Article |
| Language: | English |
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Academic Press
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115982/ http://psasir.upm.edu.my/id/eprint/115982/1/115982.pdf |
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| author | Amin, Muhamad Rahardjo, Karina Kristanti Ekarani Panosa, Alief Erwaanda Satyantini, Woro H. Mukti, Akhmad Taufiq Ali, Muhamad Musdalifah, Laila Akhyar, Halil Yanuhar, Uun Azmai, Mohammad Noor Amal Arai, Takaomi |
| author_facet | Amin, Muhamad Rahardjo, Karina Kristanti Ekarani Panosa, Alief Erwaanda Satyantini, Woro H. Mukti, Akhmad Taufiq Ali, Muhamad Musdalifah, Laila Akhyar, Halil Yanuhar, Uun Azmai, Mohammad Noor Amal Arai, Takaomi |
| author_sort | Amin, Muhamad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The etiological agent of white feces disease (WFD) infecting Pacific white shrimp Penaeus vannamei in Indonesia farms remains obscure. The present study aimed to identify possible causative agents of WFD infection in Pacific white shrimps cultured in Indonesian farms. WFD-infected and healthy samples (shrimp gut and rearing water) were collected from 8 commercial shrimp farms in East Java, Indonesia followed by bacterial community profiling using HiSeq sequencing of 16S rRNA gene amplicons. The results showed that the microbiota composition in the guts of WFD-infected shrimps was significantly different (p < 0.05) from the guts of healthy shrimps in term of genus and bacterial species. The intestinal bacterial communities of WFS-infected shrimps were overrepresented by Vibrio coralliilyticus, whereas Paracoccus was underrepresented. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States functional predictions indicated that relative abundances of 12 genes associated with the pathogenicity of bacteria including amino acid metabolisms, carbohydrate metabolisms, glycan biosynthesis, and xenobiotic biodegradation and metabolism were significantly (p < 0.05) higher in the gut microbiota of WFD-infected shrimps. These findings provide insights into the microbiome domination and their metabolic activities in the digestive tract of WFD-infected shrimps and suggest that V. coralliilyticus is a possible causative agent of WFD in cultured Indonesia Pacific white shrimp. © 2025 Elsevier Ltd |
| first_indexed | 2025-11-15T14:28:01Z |
| format | Article |
| id | upm-115982 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:28:01Z |
| publishDate | 2025 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1159822025-03-17T06:36:30Z http://psasir.upm.edu.my/id/eprint/115982/ Gut microbiota and functional metabolic predictions in white feces disease-infected Pacific white shrimp, Penaeus vannamei, from Indonesian farms Amin, Muhamad Rahardjo, Karina Kristanti Ekarani Panosa, Alief Erwaanda Satyantini, Woro H. Mukti, Akhmad Taufiq Ali, Muhamad Musdalifah, Laila Akhyar, Halil Yanuhar, Uun Azmai, Mohammad Noor Amal Arai, Takaomi The etiological agent of white feces disease (WFD) infecting Pacific white shrimp Penaeus vannamei in Indonesia farms remains obscure. The present study aimed to identify possible causative agents of WFD infection in Pacific white shrimps cultured in Indonesian farms. WFD-infected and healthy samples (shrimp gut and rearing water) were collected from 8 commercial shrimp farms in East Java, Indonesia followed by bacterial community profiling using HiSeq sequencing of 16S rRNA gene amplicons. The results showed that the microbiota composition in the guts of WFD-infected shrimps was significantly different (p < 0.05) from the guts of healthy shrimps in term of genus and bacterial species. The intestinal bacterial communities of WFS-infected shrimps were overrepresented by Vibrio coralliilyticus, whereas Paracoccus was underrepresented. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States functional predictions indicated that relative abundances of 12 genes associated with the pathogenicity of bacteria including amino acid metabolisms, carbohydrate metabolisms, glycan biosynthesis, and xenobiotic biodegradation and metabolism were significantly (p < 0.05) higher in the gut microbiota of WFD-infected shrimps. These findings provide insights into the microbiome domination and their metabolic activities in the digestive tract of WFD-infected shrimps and suggest that V. coralliilyticus is a possible causative agent of WFD in cultured Indonesia Pacific white shrimp. © 2025 Elsevier Ltd Academic Press 2025 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115982/1/115982.pdf Amin, Muhamad and Rahardjo, Karina Kristanti Ekarani and Panosa, Alief Erwaanda and Satyantini, Woro H. and Mukti, Akhmad Taufiq and Ali, Muhamad and Musdalifah, Laila and Akhyar, Halil and Yanuhar, Uun and Azmai, Mohammad Noor Amal and Arai, Takaomi (2025) Gut microbiota and functional metabolic predictions in white feces disease-infected Pacific white shrimp, Penaeus vannamei, from Indonesian farms. Fish and Shellfish Immunology, 158. art. no. 110171. pp. 1-8. ISSN 1050-4648; eISSN: 1095-9947 https://linkinghub.elsevier.com/retrieve/pii/S1050464825000609 10.1016/j.fsi.2025.110171 |
| spellingShingle | Amin, Muhamad Rahardjo, Karina Kristanti Ekarani Panosa, Alief Erwaanda Satyantini, Woro H. Mukti, Akhmad Taufiq Ali, Muhamad Musdalifah, Laila Akhyar, Halil Yanuhar, Uun Azmai, Mohammad Noor Amal Arai, Takaomi Gut microbiota and functional metabolic predictions in white feces disease-infected Pacific white shrimp, Penaeus vannamei, from Indonesian farms |
| title | Gut microbiota and functional metabolic predictions in white feces disease-infected Pacific white shrimp, Penaeus vannamei, from Indonesian farms |
| title_full | Gut microbiota and functional metabolic predictions in white feces disease-infected Pacific white shrimp, Penaeus vannamei, from Indonesian farms |
| title_fullStr | Gut microbiota and functional metabolic predictions in white feces disease-infected Pacific white shrimp, Penaeus vannamei, from Indonesian farms |
| title_full_unstemmed | Gut microbiota and functional metabolic predictions in white feces disease-infected Pacific white shrimp, Penaeus vannamei, from Indonesian farms |
| title_short | Gut microbiota and functional metabolic predictions in white feces disease-infected Pacific white shrimp, Penaeus vannamei, from Indonesian farms |
| title_sort | gut microbiota and functional metabolic predictions in white feces disease-infected pacific white shrimp, penaeus vannamei, from indonesian farms |
| url | http://psasir.upm.edu.my/id/eprint/115982/ http://psasir.upm.edu.my/id/eprint/115982/ http://psasir.upm.edu.my/id/eprint/115982/ http://psasir.upm.edu.my/id/eprint/115982/1/115982.pdf |