Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia
A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water) and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and 1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based assays, resistance to antib...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115781/ http://psasir.upm.edu.my/id/eprint/115781/1/115781.pdf |
| _version_ | 1848866859372249088 |
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| author | Radu, S. Ho, Y.K. Lihan, S. Yuherman Rusul, G. Yasin, R.M. Khair, J. Elhadi, N. |
| author_facet | Radu, S. Ho, Y.K. Lihan, S. Yuherman Rusul, G. Yasin, R.M. Khair, J. Elhadi, N. |
| author_sort | Radu, S. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water) and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and 1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based assays, resistance to antibiotics, plasmid profiles and random amplified polymorphic DNA (RAPD) analysis. All were resistant to 9 or more of the 17 antibiotics tested. Identical antibiotic resistance patterns of the isolates may indicate that they share a common mode of developing antibiotic resistance. Furthermore, the multiple antibiotic resistance indexing showed that all strains tested originated from high risk contamination. Plasmid profile analysis by agarose gel electrophoresis showed the presence of small plasmids in 12 (7 non-O1 and 5 O1 serotypes) with sizes ranging 1.3-4.6 MDa. The CT gene was detected in all clinical isolates but was present in only 14 (6 O1 serotype and 8 non-O1 serotype) isolates from environmental waters. The genetic relatedness of the clinical and environmental Vibrio cholerae O1 and non-O1 strains was investigated by RAPD fingerprinting with four primers. The four primers generated polymorphisms in all 31 strains of Vibrio cholerae tested, producing bands ranging from < 250 to 4500 bp. The RAPD profiles revealed a wide variability and no correlation with the source of isolation. This study provides evidence that Vibrio cholerae O1 and non-O1 have significant public health implications. |
| first_indexed | 2025-11-15T14:27:18Z |
| format | Article |
| id | upm-115781 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:27:18Z |
| publishDate | 1999 |
| publisher | Cambridge University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1157812025-03-12T00:56:08Z http://psasir.upm.edu.my/id/eprint/115781/ Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia Radu, S. Ho, Y.K. Lihan, S. Yuherman Rusul, G. Yasin, R.M. Khair, J. Elhadi, N. A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water) and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and 1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based assays, resistance to antibiotics, plasmid profiles and random amplified polymorphic DNA (RAPD) analysis. All were resistant to 9 or more of the 17 antibiotics tested. Identical antibiotic resistance patterns of the isolates may indicate that they share a common mode of developing antibiotic resistance. Furthermore, the multiple antibiotic resistance indexing showed that all strains tested originated from high risk contamination. Plasmid profile analysis by agarose gel electrophoresis showed the presence of small plasmids in 12 (7 non-O1 and 5 O1 serotypes) with sizes ranging 1.3-4.6 MDa. The CT gene was detected in all clinical isolates but was present in only 14 (6 O1 serotype and 8 non-O1 serotype) isolates from environmental waters. The genetic relatedness of the clinical and environmental Vibrio cholerae O1 and non-O1 strains was investigated by RAPD fingerprinting with four primers. The four primers generated polymorphisms in all 31 strains of Vibrio cholerae tested, producing bands ranging from < 250 to 4500 bp. The RAPD profiles revealed a wide variability and no correlation with the source of isolation. This study provides evidence that Vibrio cholerae O1 and non-O1 have significant public health implications. Cambridge University Press 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115781/1/115781.pdf Radu, S. and Ho, Y.K. and Lihan, S. and Yuherman and Rusul, G. and Yasin, R.M. and Khair, J. and Elhadi, N. (1999) Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia. Epidemiology and Infection, 123 (2). pp. 225-232. ISSN 0950-2688; eISSN: 0950-2688 https://www.cambridge.org/core/journals/epidemiology-and-infection/article/molecular-characterization-of-vibrio-cholerae-o1-and-nono1-from-human-and-environmental-sources-in-malaysia/FCF8A1D363D5D65FAB2F2D7C08CC9621 10.1017/S0950268899002769 |
| spellingShingle | Radu, S. Ho, Y.K. Lihan, S. Yuherman Rusul, G. Yasin, R.M. Khair, J. Elhadi, N. Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia |
| title | Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia |
| title_full | Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia |
| title_fullStr | Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia |
| title_full_unstemmed | Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia |
| title_short | Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia |
| title_sort | molecular characterization of vibrio cholerae o1 and non-o1 from human and environmental sources in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/115781/ http://psasir.upm.edu.my/id/eprint/115781/ http://psasir.upm.edu.my/id/eprint/115781/ http://psasir.upm.edu.my/id/eprint/115781/1/115781.pdf |