Different starting times of α-Tocopherol and γ-Tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer

1. α-Tocopherol (α-T) and γ-tocotrienol (γ-T) were supplemented continuously for 8 weeks in the diets of normal rats and rats chemically induced with cancer using diethylnitrosamine (DEN), 2-acetylaminofluorene (AAF) and partial hepatectomy. Hepatocarcinogenesis was followed by determining the plasm...

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Main Authors: Makpol, S., Shamaan, N.A., Jarien, Z., Md Top, A.G., Khalid, B.A.K., Wan Ngah, W.Z.
Format: Article
Language:English
Published: Elsevier Science 1997
Online Access:http://psasir.upm.edu.my/id/eprint/115780/
http://psasir.upm.edu.my/id/eprint/115780/1/115780.pdf
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author Makpol, S.
Shamaan, N.A.
Jarien, Z.
Md Top, A.G.
Khalid, B.A.K.
Wan Ngah, W.Z.
author_facet Makpol, S.
Shamaan, N.A.
Jarien, Z.
Md Top, A.G.
Khalid, B.A.K.
Wan Ngah, W.Z.
author_sort Makpol, S.
building UPM Institutional Repository
collection Online Access
description 1. α-Tocopherol (α-T) and γ-tocotrienol (γ-T) were supplemented continuously for 8 weeks in the diets of normal rats and rats chemically induced with cancer using diethylnitrosamine (DEN), 2-acetylaminofluorene (AAF) and partial hepatectomy. Hepatocarcinogenesis was followed by determining the plasma γ-glutamyl-transpeptidase (GGT) and alkaline phosphatase (ALP) activities as well as placental glutathione S transferase (PGST) and GGT activities histochemically, at 4-week intervals. 2. Male Rattus norvegicus were supplemented α-T and γ-T at two different doses of 30 and 300 mg/kg diet. The supplementation was started at three different times: simultaneously with DEN administration; 4 weeks; and 8 weeks after DEN administration. 3. Elevation of plasma GGT activities and formation of PGST and GGT positive foci were attenuated significantly (P < 0.05) when α-T and γ-T were supplemented simultaneously with cancer induction. Supplementation begun 4 and 8 weeks after cancer induction did not affect plasma enzyme activities and formation of enzyme positive foci. 4. α-T was more effective than γ-T, and a lower dose of 30 mg/kg was found to be more effective in reducing the severity of hepatocarcinogenesis.
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spelling upm-1157802025-07-10T08:01:56Z http://psasir.upm.edu.my/id/eprint/115780/ Different starting times of α-Tocopherol and γ-Tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer Makpol, S. Shamaan, N.A. Jarien, Z. Md Top, A.G. Khalid, B.A.K. Wan Ngah, W.Z. 1. α-Tocopherol (α-T) and γ-tocotrienol (γ-T) were supplemented continuously for 8 weeks in the diets of normal rats and rats chemically induced with cancer using diethylnitrosamine (DEN), 2-acetylaminofluorene (AAF) and partial hepatectomy. Hepatocarcinogenesis was followed by determining the plasma γ-glutamyl-transpeptidase (GGT) and alkaline phosphatase (ALP) activities as well as placental glutathione S transferase (PGST) and GGT activities histochemically, at 4-week intervals. 2. Male Rattus norvegicus were supplemented α-T and γ-T at two different doses of 30 and 300 mg/kg diet. The supplementation was started at three different times: simultaneously with DEN administration; 4 weeks; and 8 weeks after DEN administration. 3. Elevation of plasma GGT activities and formation of PGST and GGT positive foci were attenuated significantly (P < 0.05) when α-T and γ-T were supplemented simultaneously with cancer induction. Supplementation begun 4 and 8 weeks after cancer induction did not affect plasma enzyme activities and formation of enzyme positive foci. 4. α-T was more effective than γ-T, and a lower dose of 30 mg/kg was found to be more effective in reducing the severity of hepatocarcinogenesis. Elsevier Science 1997 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115780/1/115780.pdf Makpol, S. and Shamaan, N.A. and Jarien, Z. and Md Top, A.G. and Khalid, B.A.K. and Wan Ngah, W.Z. (1997) Different starting times of α-Tocopherol and γ-Tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer. General Pharmacology, 28 (4). pp. 589-592. ISSN 0306-3623; eISSN: 0306-3623 https://linkinghub.elsevier.com/retrieve/pii/S030636239600239X 10.1016/S0306-3623(96)00239-X
spellingShingle Makpol, S.
Shamaan, N.A.
Jarien, Z.
Md Top, A.G.
Khalid, B.A.K.
Wan Ngah, W.Z.
Different starting times of α-Tocopherol and γ-Tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer
title Different starting times of α-Tocopherol and γ-Tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer
title_full Different starting times of α-Tocopherol and γ-Tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer
title_fullStr Different starting times of α-Tocopherol and γ-Tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer
title_full_unstemmed Different starting times of α-Tocopherol and γ-Tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer
title_short Different starting times of α-Tocopherol and γ-Tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer
title_sort different starting times of α-tocopherol and γ-tocotrienol supplementation and tumor marker enzyme activities in-the rat chemically induced with cancer
url http://psasir.upm.edu.my/id/eprint/115780/
http://psasir.upm.edu.my/id/eprint/115780/
http://psasir.upm.edu.my/id/eprint/115780/
http://psasir.upm.edu.my/id/eprint/115780/1/115780.pdf