Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients
A basic glycoprotein, which was recognized by IgE from oil palm pollinosis patients, has been purified from oil palm pollen (Elaeis guineensis Jacq.), which is a strong allergen and causes severe pollinosis in Malaysia and Singapore. Soluble proteins were extracted from defatted palm pollen with bot...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press (OUP)
2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115709/ http://psasir.upm.edu.my/id/eprint/115709/1/115709.pdf |
| _version_ | 1848866846397169664 |
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| author | Kimura, Yoshinobu Maeda, Megumi Kimura, Mariko Lai, Oi Ming Tan, Siang Hee Hon, Sook Mei Chew, Fook Tim |
| author_facet | Kimura, Yoshinobu Maeda, Megumi Kimura, Mariko Lai, Oi Ming Tan, Siang Hee Hon, Sook Mei Chew, Fook Tim |
| author_sort | Kimura, Yoshinobu |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A basic glycoprotein, which was recognized by IgE from oil palm pollinosis patients, has been purified from oil palm pollen (Elaeis guineensis Jacq.), which is a strong allergen and causes severe pollinosis in Malaysia and Singapore. Soluble proteins were extracted from defatted palm pollen with both Tris-HCl buffer (pH 7.8) and Na-acetate buffer (pH 4.0). The allergenic glycoprotein was purified from the total extract to homogeneity with 0.4% yield by a combination of DEAE- and CM-cellulose, SP-HPLC, and gel filtration. The purified oil palm pollen glycoprotein with molecular mass of 31 kDa was recognized by the β1-2 xylose specific antibody, suggesting this basic glycoprotein bears plant complex type N-glycan(s). The palm pollen basic glycoprotein, designated Ela g Bd 31 K, was recognized by IgE of palm pollinosis patients, suggesting Ela g Bd 31 K should be one of the palm pollen allergens. The preliminary structural analysis of N-glycans linked to glycoproteins of palm pollens showed that the antigenic N-glycans having α1-3 fucose and β1-2 xylose residues (GlcNAc2∼0Man3Xyl1Fuc1∼0GlcNAc2) actually occur on the palm pollen glycoproteins, in addition to the high-mannose type structures (Man9∼5GlcNAc2). |
| first_indexed | 2025-11-15T14:27:05Z |
| format | Article |
| id | upm-115709 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:27:05Z |
| publishDate | 2002 |
| publisher | Oxford University Press (OUP) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1157092025-03-10T08:11:30Z http://psasir.upm.edu.my/id/eprint/115709/ Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients Kimura, Yoshinobu Maeda, Megumi Kimura, Mariko Lai, Oi Ming Tan, Siang Hee Hon, Sook Mei Chew, Fook Tim A basic glycoprotein, which was recognized by IgE from oil palm pollinosis patients, has been purified from oil palm pollen (Elaeis guineensis Jacq.), which is a strong allergen and causes severe pollinosis in Malaysia and Singapore. Soluble proteins were extracted from defatted palm pollen with both Tris-HCl buffer (pH 7.8) and Na-acetate buffer (pH 4.0). The allergenic glycoprotein was purified from the total extract to homogeneity with 0.4% yield by a combination of DEAE- and CM-cellulose, SP-HPLC, and gel filtration. The purified oil palm pollen glycoprotein with molecular mass of 31 kDa was recognized by the β1-2 xylose specific antibody, suggesting this basic glycoprotein bears plant complex type N-glycan(s). The palm pollen basic glycoprotein, designated Ela g Bd 31 K, was recognized by IgE of palm pollinosis patients, suggesting Ela g Bd 31 K should be one of the palm pollen allergens. The preliminary structural analysis of N-glycans linked to glycoproteins of palm pollens showed that the antigenic N-glycans having α1-3 fucose and β1-2 xylose residues (GlcNAc2∼0Man3Xyl1Fuc1∼0GlcNAc2) actually occur on the palm pollen glycoproteins, in addition to the high-mannose type structures (Man9∼5GlcNAc2). Oxford University Press (OUP) 2002 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115709/1/115709.pdf Kimura, Yoshinobu and Maeda, Megumi and Kimura, Mariko and Lai, Oi Ming and Tan, Siang Hee and Hon, Sook Mei and Chew, Fook Tim (2002) Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients. Bioscience, Biotechnology, and Biochemistry, 66 (4). pp. 820-827. ISSN 0916-8451; eISSN: 1347-6947 https://academic.oup.com/bbb/article/66/4/820-827/5944921 10.1271/bbb.66.820 |
| spellingShingle | Kimura, Yoshinobu Maeda, Megumi Kimura, Mariko Lai, Oi Ming Tan, Siang Hee Hon, Sook Mei Chew, Fook Tim Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients |
| title | Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients |
| title_full | Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients |
| title_fullStr | Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients |
| title_full_unstemmed | Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients |
| title_short | Purification and characterization of 31-kDa palm pollen glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients |
| title_sort | purification and characterization of 31-kda palm pollen glycoprotein (ela g bd 31 k), which is recognized by ige from palm pollinosis patients |
| url | http://psasir.upm.edu.my/id/eprint/115709/ http://psasir.upm.edu.my/id/eprint/115709/ http://psasir.upm.edu.my/id/eprint/115709/ http://psasir.upm.edu.my/id/eprint/115709/1/115709.pdf |