Physicochemical and biological characterization of the lipid particles with bovine serum albumin corona
Diacylglycerol–based nanoparticles are promising bioactive delivery systems. However, limited understanding of their interaction with biological entities restricts their clinical use. This study investigated the protein corona formed on medium and long chain diacylglycerol (MLCD)-based solid lipid n...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115557/ http://psasir.upm.edu.my/id/eprint/115557/1/115557.pdf |
| _version_ | 1848866808392581120 |
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| author | Chen, Canfeng Alfredo, Ying Ye Lee, Yee Ying Tan, Chin Ping Wang, Yong Qiu, Chaoying |
| author_facet | Chen, Canfeng Alfredo, Ying Ye Lee, Yee Ying Tan, Chin Ping Wang, Yong Qiu, Chaoying |
| author_sort | Chen, Canfeng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Diacylglycerol–based nanoparticles are promising bioactive delivery systems. However, limited understanding of their interaction with biological entities restricts their clinical use. This study investigated the protein corona formed on medium and long chain diacylglycerol (MLCD)-based solid lipid nanoparticles (NPs) modified by Polyoxethylene stearate (PEG) and compared to glyceryl tristearate (TG) and cetyl palmitate (CP) nanoparticles. Bovine serum albumin (BSA) formed corona with MLCD NPs through hydrophobic interactions and hydrogen bonding, contributing to a decrease in α–helix, an increase in β–sheet and a change in the microenvironment of Tyr residues. Owing to higher lipid hydrophilicity, MLCD NPs showed a much lower affinity for BSA than TG and CP NPs, and the binding constant with BSA was increased for larger NPs. PEG modification and the protein corona reduced the uptake of NPs by macrophages but exerted little influence on B16 cell. Among the NPs with different lipid core, the MLCD NPs showed a lower macrophages cell uptake but higher B16 cell uptake, suggesting a longer circulation time in blood but higher cancer cell internalization. This work shed light on the interactions between MLCD NPs and proteins, which is significant for application as nanocarriers with improved biological efficacy. |
| first_indexed | 2025-11-15T14:26:29Z |
| format | Article |
| id | upm-115557 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:26:29Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1155572025-04-21T02:53:45Z http://psasir.upm.edu.my/id/eprint/115557/ Physicochemical and biological characterization of the lipid particles with bovine serum albumin corona Chen, Canfeng Alfredo, Ying Ye Lee, Yee Ying Tan, Chin Ping Wang, Yong Qiu, Chaoying Diacylglycerol–based nanoparticles are promising bioactive delivery systems. However, limited understanding of their interaction with biological entities restricts their clinical use. This study investigated the protein corona formed on medium and long chain diacylglycerol (MLCD)-based solid lipid nanoparticles (NPs) modified by Polyoxethylene stearate (PEG) and compared to glyceryl tristearate (TG) and cetyl palmitate (CP) nanoparticles. Bovine serum albumin (BSA) formed corona with MLCD NPs through hydrophobic interactions and hydrogen bonding, contributing to a decrease in α–helix, an increase in β–sheet and a change in the microenvironment of Tyr residues. Owing to higher lipid hydrophilicity, MLCD NPs showed a much lower affinity for BSA than TG and CP NPs, and the binding constant with BSA was increased for larger NPs. PEG modification and the protein corona reduced the uptake of NPs by macrophages but exerted little influence on B16 cell. Among the NPs with different lipid core, the MLCD NPs showed a lower macrophages cell uptake but higher B16 cell uptake, suggesting a longer circulation time in blood but higher cancer cell internalization. This work shed light on the interactions between MLCD NPs and proteins, which is significant for application as nanocarriers with improved biological efficacy. Elsevier 2024-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115557/1/115557.pdf Chen, Canfeng and Alfredo, Ying Ye and Lee, Yee Ying and Tan, Chin Ping and Wang, Yong and Qiu, Chaoying (2024) Physicochemical and biological characterization of the lipid particles with bovine serum albumin corona. International Journal of Biological Macromolecules, 281 (pt.1). art. no. 136223. pp. 1-14. ISSN 0141-8130; eISSN: 1879-0003 https://www.sciencedirect.com/science/article/pii/S0141813024070326?via%3Dihub 10.1016/j.ijbiomac.2024.136223 |
| spellingShingle | Chen, Canfeng Alfredo, Ying Ye Lee, Yee Ying Tan, Chin Ping Wang, Yong Qiu, Chaoying Physicochemical and biological characterization of the lipid particles with bovine serum albumin corona |
| title | Physicochemical and biological characterization of the lipid particles with bovine serum albumin corona |
| title_full | Physicochemical and biological characterization of the lipid particles with bovine serum albumin corona |
| title_fullStr | Physicochemical and biological characterization of the lipid particles with bovine serum albumin corona |
| title_full_unstemmed | Physicochemical and biological characterization of the lipid particles with bovine serum albumin corona |
| title_short | Physicochemical and biological characterization of the lipid particles with bovine serum albumin corona |
| title_sort | physicochemical and biological characterization of the lipid particles with bovine serum albumin corona |
| url | http://psasir.upm.edu.my/id/eprint/115557/ http://psasir.upm.edu.my/id/eprint/115557/ http://psasir.upm.edu.my/id/eprint/115557/ http://psasir.upm.edu.my/id/eprint/115557/1/115557.pdf |