Enhanced micropropagation protocol for teak (Tectona grandis) through zygotic embryo and in vitro seedling-derived explants
This study attempted to evaluate the potential use of juvenile explants from seeds and seed-derived seedlings to establish an enhanced micropropagation protocol by optimizing the plant growth regulators that promote induction and multiplication of shoots to produce a large number of teak plantlets....
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/115522/ http://psasir.upm.edu.my/id/eprint/115522/1/115522.pdf |
| _version_ | 1848866798944911360 |
|---|---|
| author | Venkatachalam, Vasudevan Nakasha, Jaafar Juju Sinniah, Uma Rani |
| author_facet | Venkatachalam, Vasudevan Nakasha, Jaafar Juju Sinniah, Uma Rani |
| author_sort | Venkatachalam, Vasudevan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study attempted to evaluate the potential use of juvenile explants from seeds and seed-derived seedlings to establish an enhanced micropropagation protocol by optimizing the plant growth regulators that promote induction and multiplication of shoots to produce a large number of teak plantlets. In this study, seed-derived explants, such as cotyledons and zygotic embryos, and seedling-derived explants, such as shoot tips and nodal segments, were first utilized to induce multiple shoots. The maximum number of multiple shoots (9.3 shoots) with a height of 1.5 ± 0.2 cm was produced from zygotic embryos compared with shoot tips (4.3), nodal segments (4.1), and cotyledons (4.0). Approximately 9.3 shoots were obtained from explants cultured on medium supplemented with 2.0 mg L−1 BAP and 0.01 mg L−1 NAA after 5-wk incubation. Upon removal of the elongated shoot (1.5 ± 0.2 cm), the remaining clump of microshoots (< 1.0 cm) was further sub-cultured for three cycles, resulting in 775 shoots in 15 wk. The isolated shoots subjected to elongation under the optimal concentration of 0.1 mg L−1 BAP demonstrated an 80% response, accompanied by the development of shoots with a maximum length of 2.08 cm and a maximum leaf count of 5.4 after 4 wk. The rooting media supplemented with 0.01 mg L−1 NAA facilitated the highest number of roots (3.3) with an average root length of 2.6 cm, resulting in a response of 74% compared with control, which had only a 36% response and 1.8 roots with a root length of 1.6 cm. The rooted plantlets were acclimatized and then successfully transferred to the field. This efficient protocol can result in large-scale propagation of in vitro teak plantlets in the future and offer advantages in terms of healthy and resilient teak plantations. |
| first_indexed | 2025-11-15T14:26:20Z |
| format | Article |
| id | upm-115522 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:26:20Z |
| publishDate | 2024 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1155222025-04-21T02:50:18Z http://psasir.upm.edu.my/id/eprint/115522/ Enhanced micropropagation protocol for teak (Tectona grandis) through zygotic embryo and in vitro seedling-derived explants Venkatachalam, Vasudevan Nakasha, Jaafar Juju Sinniah, Uma Rani This study attempted to evaluate the potential use of juvenile explants from seeds and seed-derived seedlings to establish an enhanced micropropagation protocol by optimizing the plant growth regulators that promote induction and multiplication of shoots to produce a large number of teak plantlets. In this study, seed-derived explants, such as cotyledons and zygotic embryos, and seedling-derived explants, such as shoot tips and nodal segments, were first utilized to induce multiple shoots. The maximum number of multiple shoots (9.3 shoots) with a height of 1.5 ± 0.2 cm was produced from zygotic embryos compared with shoot tips (4.3), nodal segments (4.1), and cotyledons (4.0). Approximately 9.3 shoots were obtained from explants cultured on medium supplemented with 2.0 mg L−1 BAP and 0.01 mg L−1 NAA after 5-wk incubation. Upon removal of the elongated shoot (1.5 ± 0.2 cm), the remaining clump of microshoots (< 1.0 cm) was further sub-cultured for three cycles, resulting in 775 shoots in 15 wk. The isolated shoots subjected to elongation under the optimal concentration of 0.1 mg L−1 BAP demonstrated an 80% response, accompanied by the development of shoots with a maximum length of 2.08 cm and a maximum leaf count of 5.4 after 4 wk. The rooting media supplemented with 0.01 mg L−1 NAA facilitated the highest number of roots (3.3) with an average root length of 2.6 cm, resulting in a response of 74% compared with control, which had only a 36% response and 1.8 roots with a root length of 1.6 cm. The rooted plantlets were acclimatized and then successfully transferred to the field. This efficient protocol can result in large-scale propagation of in vitro teak plantlets in the future and offer advantages in terms of healthy and resilient teak plantations. Springer 2024-11-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115522/1/115522.pdf Venkatachalam, Vasudevan and Nakasha, Jaafar Juju and Sinniah, Uma Rani (2024) Enhanced micropropagation protocol for teak (Tectona grandis) through zygotic embryo and in vitro seedling-derived explants. In Vitro Cellular and Developmental Biology - Plant, 60. pp. 851-865. ISSN 1054-5476; eISSN: 1475-2689 https://link.springer.com/article/10.1007/s11627-024-10467-4?error=cookies_not_supported&code=b2a753e4-ef8c-4eee-9d45-1079f2d773e1 10.1007/s11627-024-10467-4 |
| spellingShingle | Venkatachalam, Vasudevan Nakasha, Jaafar Juju Sinniah, Uma Rani Enhanced micropropagation protocol for teak (Tectona grandis) through zygotic embryo and in vitro seedling-derived explants |
| title | Enhanced micropropagation protocol for teak (Tectona grandis) through zygotic embryo and in vitro seedling-derived explants |
| title_full | Enhanced micropropagation protocol for teak (Tectona grandis) through zygotic embryo and in vitro seedling-derived explants |
| title_fullStr | Enhanced micropropagation protocol for teak (Tectona grandis) through zygotic embryo and in vitro seedling-derived explants |
| title_full_unstemmed | Enhanced micropropagation protocol for teak (Tectona grandis) through zygotic embryo and in vitro seedling-derived explants |
| title_short | Enhanced micropropagation protocol for teak (Tectona grandis) through zygotic embryo and in vitro seedling-derived explants |
| title_sort | enhanced micropropagation protocol for teak (tectona grandis) through zygotic embryo and in vitro seedling-derived explants |
| url | http://psasir.upm.edu.my/id/eprint/115522/ http://psasir.upm.edu.my/id/eprint/115522/ http://psasir.upm.edu.my/id/eprint/115522/ http://psasir.upm.edu.my/id/eprint/115522/1/115522.pdf |