Natural product testing: selecting in vivo anticancer assay model

Phytochemicals and other natural products have been suggested to be effective adjuvants to conventional therapy to reduce potential side effects that arise from cancer treatments. Various natural compounds and synthetic analogues have been studied for their potential anti-cancer properties in the la...

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Main Authors: Saad, Norazalina, Chia, Suet Lin, Che Abdullah, Che Azurahanim, Sulaiman, Fadzil
Format: Article
Language:English
Published: Brawijaya University 2024
Online Access:http://psasir.upm.edu.my/id/eprint/115445/
http://psasir.upm.edu.my/id/eprint/115445/1/115445.pdf
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author Saad, Norazalina
Chia, Suet Lin
Che Abdullah, Che Azurahanim
Sulaiman, Fadzil
author_facet Saad, Norazalina
Chia, Suet Lin
Che Abdullah, Che Azurahanim
Sulaiman, Fadzil
author_sort Saad, Norazalina
building UPM Institutional Repository
collection Online Access
description Phytochemicals and other natural products have been suggested to be effective adjuvants to conventional therapy to reduce potential side effects that arise from cancer treatments. Various natural compounds and synthetic analogues have been studied for their potential anti-cancer properties in the last decade. Prior to administering them to patients in a clinical setting, drug development necessitates a sequence of preclinical testing, beginning with in vitro and progressing to in vivo studies. While numerous drugs and plant extracts were reported to be active in vitro, only several of them had reproducible activity at the tested doses in vivo. Furthermore, breakthroughs in manipulating gene editing have aided scientists in accurately replicating human diseases in animal models. Therefore, during drug development, in vivo studies are of tremendous help in evaluating a drug candidate's safety, toxicity, and efficacy in complex physiological and biochemical states. Hence, for this purpose, it is crucial to apply and select the animal model that is the most suitable to represent the studied disease or biological process. The current review summarizes various animal models that have been considered for in vivo testing, their advantages and disadvantages, and the experimental guidelines for conducting animal studies.
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spelling upm-1154452025-03-04T07:08:57Z http://psasir.upm.edu.my/id/eprint/115445/ Natural product testing: selecting in vivo anticancer assay model Saad, Norazalina Chia, Suet Lin Che Abdullah, Che Azurahanim Sulaiman, Fadzil Phytochemicals and other natural products have been suggested to be effective adjuvants to conventional therapy to reduce potential side effects that arise from cancer treatments. Various natural compounds and synthetic analogues have been studied for their potential anti-cancer properties in the last decade. Prior to administering them to patients in a clinical setting, drug development necessitates a sequence of preclinical testing, beginning with in vitro and progressing to in vivo studies. While numerous drugs and plant extracts were reported to be active in vitro, only several of them had reproducible activity at the tested doses in vivo. Furthermore, breakthroughs in manipulating gene editing have aided scientists in accurately replicating human diseases in animal models. Therefore, during drug development, in vivo studies are of tremendous help in evaluating a drug candidate's safety, toxicity, and efficacy in complex physiological and biochemical states. Hence, for this purpose, it is crucial to apply and select the animal model that is the most suitable to represent the studied disease or biological process. The current review summarizes various animal models that have been considered for in vivo testing, their advantages and disadvantages, and the experimental guidelines for conducting animal studies. Brawijaya University 2024-02-09 Article PeerReviewed text en cc_by_nc_4 http://psasir.upm.edu.my/id/eprint/115445/1/115445.pdf Saad, Norazalina and Chia, Suet Lin and Che Abdullah, Che Azurahanim and Sulaiman, Fadzil (2024) Natural product testing: selecting in vivo anticancer assay model. Journal of Tropical Life Science, 14 (1). pp. 155-172. ISSN 2087-5517; eISSN: 2527-4376 https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2852 10.11594/jtls.14.01.16
spellingShingle Saad, Norazalina
Chia, Suet Lin
Che Abdullah, Che Azurahanim
Sulaiman, Fadzil
Natural product testing: selecting in vivo anticancer assay model
title Natural product testing: selecting in vivo anticancer assay model
title_full Natural product testing: selecting in vivo anticancer assay model
title_fullStr Natural product testing: selecting in vivo anticancer assay model
title_full_unstemmed Natural product testing: selecting in vivo anticancer assay model
title_short Natural product testing: selecting in vivo anticancer assay model
title_sort natural product testing: selecting in vivo anticancer assay model
url http://psasir.upm.edu.my/id/eprint/115445/
http://psasir.upm.edu.my/id/eprint/115445/
http://psasir.upm.edu.my/id/eprint/115445/
http://psasir.upm.edu.my/id/eprint/115445/1/115445.pdf