Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection
This study investigated the effects of fish protein hydrolysate derived from barramundi on growth performance, muscle composition, immune response, disease resistance, histology and gene expression in white shrimp (Penaeus vannamei). In vitro studies demonstrated FPH enhanced mRNA expressions of key...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Academic Press
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115410/ http://psasir.upm.edu.my/id/eprint/115410/1/115410.pdf |
| _version_ | 1848866769320542208 |
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| author | Tran, Hoang Trieu Quan Ho, Thi Hang Nan, Fan-Hua Liu, Chun-Hung Hu, Yeh-Fang Chong, Chou Min de Cruz, Clement R. Karim, Murni Liu, Ting-Jui Kuo, I-Pei Lee, Po-Tsang |
| author_facet | Tran, Hoang Trieu Quan Ho, Thi Hang Nan, Fan-Hua Liu, Chun-Hung Hu, Yeh-Fang Chong, Chou Min de Cruz, Clement R. Karim, Murni Liu, Ting-Jui Kuo, I-Pei Lee, Po-Tsang |
| author_sort | Tran, Hoang Trieu Quan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study investigated the effects of fish protein hydrolysate derived from barramundi on growth performance, muscle composition, immune response, disease resistance, histology and gene expression in white shrimp (Penaeus vannamei). In vitro studies demonstrated FPH enhanced mRNA expressions of key immune-related genes and stimulated reactive oxygen species (ROS) production and phagocytic activity in shrimp hemocytes. To evaluate the effects of substituting fish meal with FPH in vivo, four isoproteic (43 %), isolipidic (6 %), and isoenergetic diets (489 kcal/100 g) were formulated with fish meal substitution levels of 0 % (control), 30 % (FPH30), 65 % (FPH65), and 100 % (FPH100). After 8-week feeding, the growth performance of FPH65 and FPH100 were significantly lower than that of control and FPH30 (p < 0.05). Similarly, the midgut histological examination revealed the wall thickness and villi height of FPH100 were significantly lower than those of control (p < 0.05). The shrimps were received the challenge of AHPND + Vibrio parahaemolyticus at week 4 and 8. All FPH-fed groups significantly enhanced resistance against Vibrio parahaemolyticus at week 4 (p < 0.05). However, this protective effect diminished after long-period feeding. No significant difference of survival rate was observed among all groups at week 8 (p > 0.05). The expressions of immune-related genes were analyzed at week 4 before and after challenge. In control group, V. parahaemolyticus significantly elevated SOD in hepatopancreas and Muc 19, trypsin, Midline-fas, and GPx in foregut (p < 0.05). Moreover, hepatopancreatic SOD of FPH65 and FPH100 were significantly higher than that of control before challenge (p < 0.05). Immune parameters were measured at week 8. Compared with control, the phagocytic index of FPH 30 was significantly higher (p < 0.05). However, dietary FPH did not alter ROS production, phenoloxidase activity, phagocytic rate, and total hemocyte count (p > 0.05). These findings suggest that FPH30 holds promise as a feed without adverse impacts on growth performance while enhancing the immunological response of white shrimp. |
| first_indexed | 2025-11-15T14:25:52Z |
| format | Article |
| id | upm-115410 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:25:52Z |
| publishDate | 2024 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1154102025-04-21T02:44:15Z http://psasir.upm.edu.my/id/eprint/115410/ Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection Tran, Hoang Trieu Quan Ho, Thi Hang Nan, Fan-Hua Liu, Chun-Hung Hu, Yeh-Fang Chong, Chou Min de Cruz, Clement R. Karim, Murni Liu, Ting-Jui Kuo, I-Pei Lee, Po-Tsang This study investigated the effects of fish protein hydrolysate derived from barramundi on growth performance, muscle composition, immune response, disease resistance, histology and gene expression in white shrimp (Penaeus vannamei). In vitro studies demonstrated FPH enhanced mRNA expressions of key immune-related genes and stimulated reactive oxygen species (ROS) production and phagocytic activity in shrimp hemocytes. To evaluate the effects of substituting fish meal with FPH in vivo, four isoproteic (43 %), isolipidic (6 %), and isoenergetic diets (489 kcal/100 g) were formulated with fish meal substitution levels of 0 % (control), 30 % (FPH30), 65 % (FPH65), and 100 % (FPH100). After 8-week feeding, the growth performance of FPH65 and FPH100 were significantly lower than that of control and FPH30 (p < 0.05). Similarly, the midgut histological examination revealed the wall thickness and villi height of FPH100 were significantly lower than those of control (p < 0.05). The shrimps were received the challenge of AHPND + Vibrio parahaemolyticus at week 4 and 8. All FPH-fed groups significantly enhanced resistance against Vibrio parahaemolyticus at week 4 (p < 0.05). However, this protective effect diminished after long-period feeding. No significant difference of survival rate was observed among all groups at week 8 (p > 0.05). The expressions of immune-related genes were analyzed at week 4 before and after challenge. In control group, V. parahaemolyticus significantly elevated SOD in hepatopancreas and Muc 19, trypsin, Midline-fas, and GPx in foregut (p < 0.05). Moreover, hepatopancreatic SOD of FPH65 and FPH100 were significantly higher than that of control before challenge (p < 0.05). Immune parameters were measured at week 8. Compared with control, the phagocytic index of FPH 30 was significantly higher (p < 0.05). However, dietary FPH did not alter ROS production, phenoloxidase activity, phagocytic rate, and total hemocyte count (p > 0.05). These findings suggest that FPH30 holds promise as a feed without adverse impacts on growth performance while enhancing the immunological response of white shrimp. Academic Press 2024-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115410/1/115410.pdf Tran, Hoang Trieu Quan and Ho, Thi Hang and Nan, Fan-Hua and Liu, Chun-Hung and Hu, Yeh-Fang and Chong, Chou Min and de Cruz, Clement R. and Karim, Murni and Liu, Ting-Jui and Kuo, I-Pei and Lee, Po-Tsang (2024) Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection. Fish and Shellfish Immunology, 150. art. no. 109597. pp. 1-12. ISSN 1050-4648; eISSN: 1095-9947 https://www.sciencedirect.com/science/article/pii/S1050464824002420?via%3Dihub 10.1016/j.fsi.2024.109597 |
| spellingShingle | Tran, Hoang Trieu Quan Ho, Thi Hang Nan, Fan-Hua Liu, Chun-Hung Hu, Yeh-Fang Chong, Chou Min de Cruz, Clement R. Karim, Murni Liu, Ting-Jui Kuo, I-Pei Lee, Po-Tsang Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection |
| title | Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection |
| title_full | Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection |
| title_fullStr | Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection |
| title_full_unstemmed | Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection |
| title_short | Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection |
| title_sort | assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: impact on growth, immune response, and resistance against vibrio parahaemolyticus infection |
| url | http://psasir.upm.edu.my/id/eprint/115410/ http://psasir.upm.edu.my/id/eprint/115410/ http://psasir.upm.edu.my/id/eprint/115410/ http://psasir.upm.edu.my/id/eprint/115410/1/115410.pdf |