Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells
In this study, blackberry polysaccharide-selenium nanoparticles (BBP-24-3Se) were first prepared via Na2SeO3/Vc redox reaction, followed by coating with red blood cell membrane (RBC) to form core–shell structure polysaccharide-selenium nanoparticles (RBC@BBP-24-3Se). The particle size of BBP-24-3Se...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115386/ http://psasir.upm.edu.my/id/eprint/115386/1/115386.pdf |
| _version_ | 1848866763337367552 |
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| author | Zu-Man, Dou Yu-Long, Zhang Chun-Yang, Tang Chuang, Liu Jia-Qin, Fang Qiang, Huang Chun, Chen Li-Jun, You Chin-Ping, Tan Hui, Niu Xiong, Fu |
| author_facet | Zu-Man, Dou Yu-Long, Zhang Chun-Yang, Tang Chuang, Liu Jia-Qin, Fang Qiang, Huang Chun, Chen Li-Jun, You Chin-Ping, Tan Hui, Niu Xiong, Fu |
| author_sort | Zu-Man, Dou |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, blackberry polysaccharide-selenium nanoparticles (BBP-24-3Se) were first prepared via Na2SeO3/Vc redox reaction, followed by coating with red blood cell membrane (RBC) to form core–shell structure polysaccharide-selenium nanoparticles (RBC@BBP-24-3Se). The particle size of BBP-24-3Se (167.1 nm) was increased to 239.8 nm (RBC@BBP-24-3Se) with an obvious core–shell structure after coating with RBC. FT-IR and XPS results indicated that the interaction between BBP-24-3 and SeNPs formed a new C-O···Se bond with valence state of Se0. Bioassays indicated that RBC coating markedly enhanced both the biocompatibility and bioabsorbability of RBC@BBP-24-3Se, and the absorption rate of RBC@BBP-24-3Se in HepG2 cells was 4.99 times higher than that of BBP-24-3Se at a concentration of 10 μg/mL. Compared with BBP-24-3Se, RBC@BBP-24-3Se possessed significantly heightened protective efficacy against oxidative damage and better regulation of glucose/lipid metabolism disorder induced by palmitic acid in HepG2 cells. Mechanistic studies demonstrated that RBC@BBP-24-3Se could effectively improve PI3K/AKT signaling pathway to promote glucose metabolism, inhibit the expression of lipid synthesis genes and up-regulate the expression of lipid-decomposing genes through AMPK signaling pathway to improve lipid metabolism. These results provided a theoretical basis for developing a new type of selenium supplement for the treatment of insulin resistance. |
| first_indexed | 2025-11-15T14:25:46Z |
| format | Article |
| id | upm-115386 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:25:46Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1153862025-04-21T02:40:52Z http://psasir.upm.edu.my/id/eprint/115386/ Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells Zu-Man, Dou Yu-Long, Zhang Chun-Yang, Tang Chuang, Liu Jia-Qin, Fang Qiang, Huang Chun, Chen Li-Jun, You Chin-Ping, Tan Hui, Niu Xiong, Fu In this study, blackberry polysaccharide-selenium nanoparticles (BBP-24-3Se) were first prepared via Na2SeO3/Vc redox reaction, followed by coating with red blood cell membrane (RBC) to form core–shell structure polysaccharide-selenium nanoparticles (RBC@BBP-24-3Se). The particle size of BBP-24-3Se (167.1 nm) was increased to 239.8 nm (RBC@BBP-24-3Se) with an obvious core–shell structure after coating with RBC. FT-IR and XPS results indicated that the interaction between BBP-24-3 and SeNPs formed a new C-O···Se bond with valence state of Se0. Bioassays indicated that RBC coating markedly enhanced both the biocompatibility and bioabsorbability of RBC@BBP-24-3Se, and the absorption rate of RBC@BBP-24-3Se in HepG2 cells was 4.99 times higher than that of BBP-24-3Se at a concentration of 10 μg/mL. Compared with BBP-24-3Se, RBC@BBP-24-3Se possessed significantly heightened protective efficacy against oxidative damage and better regulation of glucose/lipid metabolism disorder induced by palmitic acid in HepG2 cells. Mechanistic studies demonstrated that RBC@BBP-24-3Se could effectively improve PI3K/AKT signaling pathway to promote glucose metabolism, inhibit the expression of lipid synthesis genes and up-regulate the expression of lipid-decomposing genes through AMPK signaling pathway to improve lipid metabolism. These results provided a theoretical basis for developing a new type of selenium supplement for the treatment of insulin resistance. Elsevier 2024-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115386/1/115386.pdf Zu-Man, Dou and Yu-Long, Zhang and Chun-Yang, Tang and Chuang, Liu and Jia-Qin, Fang and Qiang, Huang and Chun, Chen and Li-Jun, You and Chin-Ping, Tan and Hui, Niu and Xiong, Fu (2024) Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells. Food Research International, 187. art. no. 114428. ISSN 0963-9969; eISSN: 1873-7145 https://www.sciencedirect.com/science/article/pii/S0963996924004988?via%3Dihub 10.1016/j.foodres.2024.114428 |
| spellingShingle | Zu-Man, Dou Yu-Long, Zhang Chun-Yang, Tang Chuang, Liu Jia-Qin, Fang Qiang, Huang Chun, Chen Li-Jun, You Chin-Ping, Tan Hui, Niu Xiong, Fu Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells |
| title | Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells |
| title_full | Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells |
| title_fullStr | Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells |
| title_full_unstemmed | Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells |
| title_short | Construction of blackberry polysaccharide nano-selenium particles: Structure features and regulation effects of glucose/lipid metabolism in HepG2 cells |
| title_sort | construction of blackberry polysaccharide nano-selenium particles: structure features and regulation effects of glucose/lipid metabolism in hepg2 cells |
| url | http://psasir.upm.edu.my/id/eprint/115386/ http://psasir.upm.edu.my/id/eprint/115386/ http://psasir.upm.edu.my/id/eprint/115386/ http://psasir.upm.edu.my/id/eprint/115386/1/115386.pdf |