Enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism
ω-3 fatty acids-based phospholipids (ω-3-PLs) exhibit higher bioavailability within organisms and stability compared to polyunsaturated fatty acids in their neutral forms. In this study, ω-3 fatty acids enriched partial acylglycerols (ω-3-AGs) were produced from hydrolysis of algal oil firstly. The...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115355/ http://psasir.upm.edu.my/id/eprint/115355/1/115355.pdf |
| _version_ | 1848866755156377600 |
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| author | Wu, Yuxin Peng, Xianwu Lee, Yee-Ying Xie, Pengkai Tan, Chin-Ping Wang, Yong Zhang, Zhen |
| author_facet | Wu, Yuxin Peng, Xianwu Lee, Yee-Ying Xie, Pengkai Tan, Chin-Ping Wang, Yong Zhang, Zhen |
| author_sort | Wu, Yuxin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | ω-3 fatty acids-based phospholipids (ω-3-PLs) exhibit higher bioavailability within organisms and stability compared to polyunsaturated fatty acids in their neutral forms. In this study, ω-3 fatty acids enriched partial acylglycerols (ω-3-AGs) were produced from hydrolysis of algal oil firstly. The effects of hydrolysis processing parameters such as enzyme type, enzyme loading, oil-water ratio, reaction temperature, and time were evaluated. Subsequently, ω-3-PLs were prepared via interesterification of ω-3-AGs with PLs catalyzed by PLA1. When monoacylglycerols (MAGs) reached 60% in partial acylglycerols, the interesterification reaction showed a marked improvement, leading to the achievement of the optimal final ω-3-PLs content of 38.93%. Using molecular docking techniques to speculate on the catalytic mechanism, the results indicate that MAGs exhibit an affinity of up to −6.33 kcal/mol with PLA1, verifying their critical role in the interesterification process. |
| first_indexed | 2025-11-15T14:25:38Z |
| format | Article |
| id | upm-115355 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:25:38Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1153552025-04-21T02:37:38Z http://psasir.upm.edu.my/id/eprint/115355/ Enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism Wu, Yuxin Peng, Xianwu Lee, Yee-Ying Xie, Pengkai Tan, Chin-Ping Wang, Yong Zhang, Zhen ω-3 fatty acids-based phospholipids (ω-3-PLs) exhibit higher bioavailability within organisms and stability compared to polyunsaturated fatty acids in their neutral forms. In this study, ω-3 fatty acids enriched partial acylglycerols (ω-3-AGs) were produced from hydrolysis of algal oil firstly. The effects of hydrolysis processing parameters such as enzyme type, enzyme loading, oil-water ratio, reaction temperature, and time were evaluated. Subsequently, ω-3-PLs were prepared via interesterification of ω-3-AGs with PLs catalyzed by PLA1. When monoacylglycerols (MAGs) reached 60% in partial acylglycerols, the interesterification reaction showed a marked improvement, leading to the achievement of the optimal final ω-3-PLs content of 38.93%. Using molecular docking techniques to speculate on the catalytic mechanism, the results indicate that MAGs exhibit an affinity of up to −6.33 kcal/mol with PLA1, verifying their critical role in the interesterification process. Elsevier 2024-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115355/1/115355.pdf Wu, Yuxin and Peng, Xianwu and Lee, Yee-Ying and Xie, Pengkai and Tan, Chin-Ping and Wang, Yong and Zhang, Zhen (2024) Enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism. Food Bioscience, 62. art. no. 105246. pp. 1-10. ISSN 2212-4292; eISSN: 2212-4306 https://www.sciencedirect.com/science/article/pii/S2212429224016766?via%3Dihub 10.1016/j.fbio.2024.105246 |
| spellingShingle | Wu, Yuxin Peng, Xianwu Lee, Yee-Ying Xie, Pengkai Tan, Chin-Ping Wang, Yong Zhang, Zhen Enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism |
| title | Enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism |
| title_full | Enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism |
| title_fullStr | Enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism |
| title_full_unstemmed | Enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism |
| title_short | Enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism |
| title_sort | enzymatic synthesis of ω-3 fatty acids-based structured phospholipids: a novel solvent-free preparation approach and its catalytic mechanism |
| url | http://psasir.upm.edu.my/id/eprint/115355/ http://psasir.upm.edu.my/id/eprint/115355/ http://psasir.upm.edu.my/id/eprint/115355/ http://psasir.upm.edu.my/id/eprint/115355/1/115355.pdf |