Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli
The coding region of the nucleocapsid (N) gene was amplified from the viral RNA and inserted into the bacterial expression vector, pTrcHis2, for intracellular expression in three Escherichia coli strains: TOP 10, BL 21 and SG 935. The N protein was expressed as a fusion protein containing the myc ep...
| Main Authors: | Ong, S.T., Tan, W.S., Hassan, S.S., Mohd Lila, M.A. |
|---|---|
| Format: | Article |
| Published: |
Taylor & Francis
2002
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/115312/ |
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