Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable met...
| Main Authors: | Eskandari, Azadeh, Nezhad, Nima Ghahremani, Leow, Thean Chor, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya |
|---|---|
| Format: | Article |
| Published: |
Springer Science and Business Media Deutschland GmbH
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/115291/ |
Similar Items
Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
by: Eskandari, Azadeh, et al.
Published: (2024)
by: Eskandari, Azadeh, et al.
Published: (2024)
Advances in therapeutic cancer vaccines, their obstacles, and prospects toward tumor immunotherapy
by: Eskandari, Azadeh, et al.
Published: (2024)
by: Eskandari, Azadeh, et al.
Published: (2024)
Recent insight into the advances and prospects of microbial lipases and their potential applications in industry
by: Eskandari, Azadeh, et al.
Published: (2024)
by: Eskandari, Azadeh, et al.
Published: (2024)
Advanced freezing point insights into regulatory role of antifreeze proteins, their fundamentals, and obstacles in food preservation
by: Eskandari, Azadeh, et al.
Published: (2024)
by: Eskandari, Azadeh, et al.
Published: (2024)
Recent advances in overexpression of functional recombinant lipases
by: Alias, Fatin Liyana, et al.
Published: (2023)
by: Alias, Fatin Liyana, et al.
Published: (2023)
Molecular dynamics-guided insight into the adsorption–inhibition mechanism for controlling ice growth/melt of antifreeze protein type IV mutant from longhorn sculpin fish
by: Eskandari, Azadeh, et al.
Published: (2024)
by: Eskandari, Azadeh, et al.
Published: (2024)
Structural investigation, computational analysis, and theoretical cryoprotectant approach of antifreeze protein type IV mutants
by: Eskandari, Azadeh, et al.
Published: (2024)
by: Eskandari, Azadeh, et al.
Published: (2024)
Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b
by: Heidary, Somayyeh, et al.
Published: (2014)
by: Heidary, Somayyeh, et al.
Published: (2014)
Expression and the antigenicity of recombinant coat proteins of tungro viruses expressed in Escherichia coli
by: Yee, Siewfung, et al.
Published: (2017)
by: Yee, Siewfung, et al.
Published: (2017)
Recent advances in simultaneous thermostability-activity improvement of industrial enzymes through structure modification
by: Nezhad, Nima Ghahremani, et al.
Published: (2023)
by: Nezhad, Nima Ghahremani, et al.
Published: (2023)
Cold-active lipases and esterases: a review on recombinant overexpression and other essential issues
by: Matinja, Adamu Idris, et al.
Published: (2022)
by: Matinja, Adamu Idris, et al.
Published: (2022)
Cloning and purification of recombinant proteins of Mycoplasma hyopneumoniae expressed in Escherichia coli
by: Simionatto, S., et al.
Published: (2010)
by: Simionatto, S., et al.
Published: (2010)
Isolation, Cloning and Expression of Recombinant Human Renin in Escherichia Coli System
by: Ng, Chyan Leong
Published: (2002)
by: Ng, Chyan Leong
Published: (2002)
Cloning and Expression of Recombinant Human Epidermal Growth Factor In Escherichia Coli
by: Abdull Razis, Ahmad Faizal
Published: (2007)
by: Abdull Razis, Ahmad Faizal
Published: (2007)
Use of a newly isolated yeast in the expression of recombinant proteins
by: Salleh, Abu Bakar, et al.
Published: (2012)
by: Salleh, Abu Bakar, et al.
Published: (2012)
A newly isolated yeast as an expression host for recombinant lipase
by: Oslan, Siti Nurbaya, et al.
Published: (2015)
by: Oslan, Siti Nurbaya, et al.
Published: (2015)
Optimization of inoculum size & agitation rate on expression of recombinant chitinase in Escherichia coli
by: Nor Fadzillah Hanis, Abu Sulaiman
Published: (2009)
by: Nor Fadzillah Hanis, Abu Sulaiman
Published: (2009)
Periplasmic protein changes in response to over-expression of recombinant protein in escherichia coli
by: Heidary, Somayyeh
Published: (2013)
by: Heidary, Somayyeh
Published: (2013)
Expression and characterization of Geobacillus stearothermophilus SR74 recombinant a-Amylase in Pichia pastoris
by: Gandhi, Sivasangkary, et al.
Published: (2015)
by: Gandhi, Sivasangkary, et al.
Published: (2015)
Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology
by: Norheziwan, Hamsah
Published: (2009)
by: Norheziwan, Hamsah
Published: (2009)
Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli
by: Zakaria, Iffah Izzati, et al.
Published: (2011)
by: Zakaria, Iffah Izzati, et al.
Published: (2011)
Molecular expression of a recombinant thermostable bacterial amylase from geobacillus stearothermophilus SR74 using methanol-free meyerozyma guilliermondii strain SO yeast system
by: Mohamad Nasir, Nurul Syazwani, et al.
Published: (2020)
by: Mohamad Nasir, Nurul Syazwani, et al.
Published: (2020)
Heterologous expression of Bromelain in Escherichia coli
by: M., Bala, et al.
Published: (2011)
by: M., Bala, et al.
Published: (2011)
Immunogenic properties of recombinant ectodomain of Newcastle disease virus hemagglutinin-neuraminidase protein expressed in Escherichia coli.
by: Wong, Sing King, et al.
Published: (2009)
by: Wong, Sing King, et al.
Published: (2009)
Periplasmic Expression, Recovery and Quantification of
Recombinant Human Interferon-A2b in Fermentation by Escherichia Coli
by: Ramanan, R. Nagasundara
Published: (2009)
by: Ramanan, R. Nagasundara
Published: (2009)
Heterologous expression of CGT-BS gene improved recombinant cyclodextrin glycosyltransferase production from Escherichia coli
by: Nik Pa, Nik Ida Mardiana, et al.
Published: (2017)
by: Nik Pa, Nik Ida Mardiana, et al.
Published: (2017)
Optimization of medium formulation on production of recombinant chitinase in escherichia coli
by: Mohd Faris, Mohd Yunus
Published: (2010)
by: Mohd Faris, Mohd Yunus
Published: (2010)
Production of cyclodextrin (cd) using immobilized recombinant Escherichia coli
by: Rohaida, Che Man, et al.
Published: (2021)
by: Rohaida, Che Man, et al.
Published: (2021)
Development and characterisation of a recombinant Escherichia coli for the production of Polyhydroxyalkanoates
by: Yee, Lian Ngit
Published: (2013)
by: Yee, Lian Ngit
Published: (2013)
Functional expression, purification, biochemical and biophysical characterizations, and molecular dynamics simulation of a histidine acid phosphatase from Saccharomyces cerevisiae
by: Nezhad, Nima Ghahremani, et al.
Published: (2024)
by: Nezhad, Nima Ghahremani, et al.
Published: (2024)
Functional expression, purification, biochemical and biophysical characterizations, and molecular dynamics simulation of a histidine acid phosphatase from Saccharomyces cerevisiae
by: Nezhad, Nima Ghahremani, et al.
Published: (2024)
by: Nezhad, Nima Ghahremani, et al.
Published: (2024)
Plasmid-based and genome-based expression of recombinant T1 lipase in sucrose-utilizing E. coli strain W
by: Yusof, Siti Hajar, et al.
Published: (2024)
by: Yusof, Siti Hajar, et al.
Published: (2024)
Isolation, screening and molecular characterization of phytase producing microorganisms to discover the novel phytase
by: Nezhad, Nima Ghahremani, et al.
Published: (2023)
by: Nezhad, Nima Ghahremani, et al.
Published: (2023)
Recovery of recombinant bromelain from Escherichia
coli BL21-AI
by: Bala, Muntari, et al.
Published: (2011)
by: Bala, Muntari, et al.
Published: (2011)
Cell rupture of recombinant escherichia coli using high pressure homogenizer
by: Ahmad Raus, Raha, et al.
Published: (2010)
by: Ahmad Raus, Raha, et al.
Published: (2010)
Cell rupture of recombinant escherichia coli using high pressure homogenizer
by: Ahmad Raus, Raha, et al.
Published: (2009)
by: Ahmad Raus, Raha, et al.
Published: (2009)
Crossflow microfiltration of recombinant Escherichia coli producing β-glucuronidase enzyme
by: Mel, Maizirwan, et al.
Published: (2011)
by: Mel, Maizirwan, et al.
Published: (2011)
Effect of Process Parameters on Immobilization of Recombinant Escherichia coli on Coconut Fiber
by: Rohaida, Che Man, et al.
Published: (2024)
by: Rohaida, Che Man, et al.
Published: (2024)
Hydrogen and ethanol co-production by recombinant Escherichia coli SS1
by: Soo, Chiu Shyan
Published: (2016)
by: Soo, Chiu Shyan
Published: (2016)
Thermostability engineering of industrial enzymes through structure modification
by: Nezhad, Nima Ghahremani, et al.
Published: (2022)
by: Nezhad, Nima Ghahremani, et al.
Published: (2022)
Similar Items
-
Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
by: Eskandari, Azadeh, et al.
Published: (2024) -
Advances in therapeutic cancer vaccines, their obstacles, and prospects toward tumor immunotherapy
by: Eskandari, Azadeh, et al.
Published: (2024) -
Recent insight into the advances and prospects of microbial lipases and their potential applications in industry
by: Eskandari, Azadeh, et al.
Published: (2024) -
Advanced freezing point insights into regulatory role of antifreeze proteins, their fundamentals, and obstacles in food preservation
by: Eskandari, Azadeh, et al.
Published: (2024) -
Recent advances in overexpression of functional recombinant lipases
by: Alias, Fatin Liyana, et al.
Published: (2023)