Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable met...
| Main Authors: | , , , , |
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| Format: | Article |
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Springer Science and Business Media Deutschland GmbH
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115291/ |
| _version_ | 1848866737754210304 |
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| author | Eskandari, Azadeh Nezhad, Nima Ghahremani Leow, Thean Chor Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya |
| author_facet | Eskandari, Azadeh Nezhad, Nima Ghahremani Leow, Thean Chor Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya |
| author_sort | Eskandari, Azadeh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable method for biotherapeutics and food industry productions. Hence, it is crucial to analyze the limitations and advantages of microbial hosts to enhance the efficient production of recombinant proteins on a large scale. E. coli is widely used as a host for the production of recombinant proteins. Researchers have identified certain obstacles with this host, and given the growing demand for recombinant protein production, there is an immediate requirement to enhance this host. The following review discusses the elements contributing to the manifestation of recombinant protein. Subsequently, it sheds light on innovative approaches aimed at improving the expression of recombinant protein. Lastly, it delves into the obstacles and optimization methods associated with translation, mentioning both cis-optimization and trans-optimization, producing soluble recombinant protein, and engineering the metal ion transportation. In this context, a comprehensive description of the distinct features will be provided, and this knowledge could potentially enhance the expression of recombinant proteins in E. coli. |
| first_indexed | 2025-11-15T14:25:22Z |
| format | Article |
| id | upm-115291 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:25:22Z |
| publishDate | 2024 |
| publisher | Springer Science and Business Media Deutschland GmbH |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1152912025-02-28T01:43:35Z http://psasir.upm.edu.my/id/eprint/115291/ Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli Eskandari, Azadeh Nezhad, Nima Ghahremani Leow, Thean Chor Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable method for biotherapeutics and food industry productions. Hence, it is crucial to analyze the limitations and advantages of microbial hosts to enhance the efficient production of recombinant proteins on a large scale. E. coli is widely used as a host for the production of recombinant proteins. Researchers have identified certain obstacles with this host, and given the growing demand for recombinant protein production, there is an immediate requirement to enhance this host. The following review discusses the elements contributing to the manifestation of recombinant protein. Subsequently, it sheds light on innovative approaches aimed at improving the expression of recombinant protein. Lastly, it delves into the obstacles and optimization methods associated with translation, mentioning both cis-optimization and trans-optimization, producing soluble recombinant protein, and engineering the metal ion transportation. In this context, a comprehensive description of the distinct features will be provided, and this knowledge could potentially enhance the expression of recombinant proteins in E. coli. Springer Science and Business Media Deutschland GmbH 2024-03-12 Article PeerReviewed Eskandari, Azadeh and Nezhad, Nima Ghahremani and Leow, Thean Chor and Abdul Rahman, Mohd Basyaruddin and Oslan, Siti Nurbaya (2024) Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli. Archives of Microbiology, 206 (4). art. no. 152. ISSN 0302-8933; eISSN: 1432-072X https://link.springer.com/article/10.1007/s00203-024-03871-2 10.1007/s00203-024-03871-2 |
| spellingShingle | Eskandari, Azadeh Nezhad, Nima Ghahremani Leow, Thean Chor Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli |
| title | Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli |
| title_full | Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli |
| title_fullStr | Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli |
| title_full_unstemmed | Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli |
| title_short | Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli |
| title_sort | essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/115291/ http://psasir.upm.edu.my/id/eprint/115291/ http://psasir.upm.edu.my/id/eprint/115291/ |