Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum
A pair of PCR primers for the rapid detection of bile salt hydrolase (bsh) gene from Bifidobacterium longum BB536 has been synthesised and have revealed the bsh gene of approx 970 bp in Bifidobacterium longum BB 536 but not in other species of bacteria tested. The bsh gene was cloned and sequenced s...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
2001
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/115211/ http://psasir.upm.edu.my/id/eprint/115211/1/115211.pdf |
| _version_ | 1848866715578925056 |
|---|---|
| author | Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. |
| author_facet | Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. |
| author_sort | Shuhaimi, M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A pair of PCR primers for the rapid detection of bile salt hydrolase (bsh) gene from Bifidobacterium longum BB536 has been synthesised and have revealed the bsh gene of approx 970 bp in Bifidobacterium longum BB 536 but not in other species of bacteria tested. The bsh gene was cloned and sequenced showing a high similarity to bsh gene previously published. The resulting nucleotide sequence encodes a predicted protein of 317 amino acids, Mw = 35 kDa. |
| first_indexed | 2025-11-15T14:25:01Z |
| format | Article |
| id | upm-115211 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:25:01Z |
| publishDate | 2001 |
| publisher | Springer Science and Business Media LLC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1152112025-02-26T03:52:29Z http://psasir.upm.edu.my/id/eprint/115211/ Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. A pair of PCR primers for the rapid detection of bile salt hydrolase (bsh) gene from Bifidobacterium longum BB536 has been synthesised and have revealed the bsh gene of approx 970 bp in Bifidobacterium longum BB 536 but not in other species of bacteria tested. The bsh gene was cloned and sequenced showing a high similarity to bsh gene previously published. The resulting nucleotide sequence encodes a predicted protein of 317 amino acids, Mw = 35 kDa. Springer Science and Business Media LLC 2001 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115211/1/115211.pdf Shuhaimi, M. and Ali, A.M. and Saleh, N.M. and Yazid, A.M. (2001) Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum. Biotechnology Letters, 23 (21). pp. 1775-1780. ISSN 0141-5492 https://link.springer.com/article/10.1023/A:1012400518207?error=cookies_not_supported&code=b74611f1-a6e5-471f-9e24-c02e186de39a 10.1023/A:1012400518207 |
| spellingShingle | Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum |
| title | Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum |
| title_full | Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum |
| title_fullStr | Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum |
| title_full_unstemmed | Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum |
| title_short | Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum |
| title_sort | cloning and sequence analysis of bile salt hydrolase (bsh) gene from bifidobacterium longum |
| url | http://psasir.upm.edu.my/id/eprint/115211/ http://psasir.upm.edu.my/id/eprint/115211/ http://psasir.upm.edu.my/id/eprint/115211/ http://psasir.upm.edu.my/id/eprint/115211/1/115211.pdf |