3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum
The effect of the C-serum (the cytosol) on the activity of 3-hydroxy-3-methylglutaryl CoA reductase in the latex of Hevea brasiliensis was investigated. Depending on the clone from which the latex was obtained, the C-serum was found to depress or activate or have little effect on the enzyme activity...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
1982
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115115/ http://psasir.upm.edu.my/id/eprint/115115/1/115115.pdf |
| _version_ | 1848866689436876800 |
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| author | Mohd. Isa, Ramli B. Sipat, A.B. |
| author_facet | Mohd. Isa, Ramli B. Sipat, A.B. |
| author_sort | Mohd. Isa, Ramli B. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The effect of the C-serum (the cytosol) on the activity of 3-hydroxy-3-methylglutaryl CoA reductase in the latex of Hevea brasiliensis was investigated. Depending on the clone from which the latex was obtained, the C-serum was found to depress or activate or have little effect on the enzyme activity. Boiling the C-serum however, resulted in a consistent activation effect in all the clones examined. Optimal activation was obtained with 20 μl boiled C-serum. Dialysis or EDTA (40 mM) treatment of the boiled C-serum did not diminish the activation effect. Although not essential, dithiothreitol complemented the activation effect of the boiled C-serum and the optimal concentration was 10 mM. Trypsin digestion of the boiled C-serum resulted in the complete loss of the activation effect. The activator in the boiled C-serum was salted out by ammonium sulphate at 25 - 100% saturation. Hevein had no effect on reductase activity. |
| first_indexed | 2025-11-15T14:24:36Z |
| format | Article |
| id | upm-115115 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:24:36Z |
| publishDate | 1982 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1151152025-02-20T07:15:38Z http://psasir.upm.edu.my/id/eprint/115115/ 3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum Mohd. Isa, Ramli B. Sipat, A.B. The effect of the C-serum (the cytosol) on the activity of 3-hydroxy-3-methylglutaryl CoA reductase in the latex of Hevea brasiliensis was investigated. Depending on the clone from which the latex was obtained, the C-serum was found to depress or activate or have little effect on the enzyme activity. Boiling the C-serum however, resulted in a consistent activation effect in all the clones examined. Optimal activation was obtained with 20 μl boiled C-serum. Dialysis or EDTA (40 mM) treatment of the boiled C-serum did not diminish the activation effect. Although not essential, dithiothreitol complemented the activation effect of the boiled C-serum and the optimal concentration was 10 mM. Trypsin digestion of the boiled C-serum resulted in the complete loss of the activation effect. The activator in the boiled C-serum was salted out by ammonium sulphate at 25 - 100% saturation. Hevein had no effect on reductase activity. Elsevier BV 1982 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115115/1/115115.pdf Mohd. Isa, Ramli B. and Sipat, A.B. (1982) 3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum. Biochemical and Biophysical Research Communications, 108 (1). pp. 206-212. ISSN 0006-291X https://linkinghub.elsevier.com/retrieve/pii/0006291X82918526 10.1016/0006-291X(82)91852-6 |
| spellingShingle | Mohd. Isa, Ramli B. Sipat, A.B. 3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum |
| title | 3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum |
| title_full | 3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum |
| title_fullStr | 3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum |
| title_full_unstemmed | 3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum |
| title_short | 3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum |
| title_sort | 3-hydroxy-3-methylglutaryl coa reductase of hevea latex: the occurrence of a heat-stable activator in the c-serum |
| url | http://psasir.upm.edu.my/id/eprint/115115/ http://psasir.upm.edu.my/id/eprint/115115/ http://psasir.upm.edu.my/id/eprint/115115/ http://psasir.upm.edu.my/id/eprint/115115/1/115115.pdf |