Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 μM in 25% methanol can catalyse the synthetic reaction. The op...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
1992
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/115092/ http://psasir.upm.edu.my/id/eprint/115092/1/115092.pdf |
| _version_ | 1848866683065729024 |
|---|---|
| author | Lee, Kong H. Lee, Pat M. Siaw, Y.S. Morihara, Kazuyuki |
| author_facet | Lee, Kong H. Lee, Pat M. Siaw, Y.S. Morihara, Kazuyuki |
| author_sort | Lee, Kong H. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 μM in 25% methanol can catalyse the synthetic reaction. The optimum methanol compositions at 4°C and 37°C were 50% and 25% respectively where an increase in peptide yield of 85% was obtained for both conditions as compared to that in water. |
| first_indexed | 2025-11-15T14:24:30Z |
| format | Article |
| id | upm-115092 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:24:30Z |
| publishDate | 1992 |
| publisher | Springer Science and Business Media LLC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1150922025-02-19T07:44:35Z http://psasir.upm.edu.my/id/eprint/115092/ Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin Lee, Kong H. Lee, Pat M. Siaw, Y.S. Morihara, Kazuyuki The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 μM in 25% methanol can catalyse the synthetic reaction. The optimum methanol compositions at 4°C and 37°C were 50% and 25% respectively where an increase in peptide yield of 85% was obtained for both conditions as compared to that in water. Springer Science and Business Media LLC 1992 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115092/1/115092.pdf Lee, Kong H. and Lee, Pat M. and Siaw, Y.S. and Morihara, Kazuyuki (1992) Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin. Biotechnology Letters, 14 (12). pp. 1159-1164. ISSN 0141-5492; eISSN: 1573-6776 https://link.springer.com/article/10.1007/BF01027021?error=cookies_not_supported&code=d1721d86-f388-468c-9b3a-08448006333f 10.1007/BF01027021 |
| spellingShingle | Lee, Kong H. Lee, Pat M. Siaw, Y.S. Morihara, Kazuyuki Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin |
| title | Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin |
| title_full | Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin |
| title_fullStr | Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin |
| title_full_unstemmed | Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin |
| title_short | Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin |
| title_sort | aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin |
| url | http://psasir.upm.edu.my/id/eprint/115092/ http://psasir.upm.edu.my/id/eprint/115092/ http://psasir.upm.edu.my/id/eprint/115092/ http://psasir.upm.edu.my/id/eprint/115092/1/115092.pdf |