Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation
Nylon tube was directly activated by alkaline glutaraldehyde solution. PEI was utilised as a spacer molecule. Glucose oxidase was immobilised to the nylon tube after reactivating the spacer molecules with glutaraldehyde. On immobilising glucose oxidase there was more protein binding and higher immob...
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| Format: | Article |
| Language: | English |
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Springer Science and Business Media LLC
1982
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115073/ http://psasir.upm.edu.my/id/eprint/115073/1/115073.pdf |
| _version_ | 1848866677441167360 |
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| author | Salleh, Abu Bakar |
| author_facet | Salleh, Abu Bakar |
| author_sort | Salleh, Abu Bakar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nylon tube was directly activated by alkaline glutaraldehyde solution. PEI was utilised as a spacer molecule. Glucose oxidase was immobilised to the nylon tube after reactivating the spacer molecules with glutaraldehyde. On immobilising glucose oxidase there was more protein binding and higher immobilised enzyme activity when compared to immobilised enzyme tube activated by triethyloxonium salt. The optimal condition for direct glutaraldehyde activation of nylon was incubation with 18.5% (w/v) glutaraldehyde in 0.12M borate pH 9.0 for 15 min at 90 °. |
| first_indexed | 2025-11-15T14:24:24Z |
| format | Article |
| id | upm-115073 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:24:24Z |
| publishDate | 1982 |
| publisher | Springer Science and Business Media LLC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1150732025-02-19T07:25:30Z http://psasir.upm.edu.my/id/eprint/115073/ Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation Salleh, Abu Bakar Nylon tube was directly activated by alkaline glutaraldehyde solution. PEI was utilised as a spacer molecule. Glucose oxidase was immobilised to the nylon tube after reactivating the spacer molecules with glutaraldehyde. On immobilising glucose oxidase there was more protein binding and higher immobilised enzyme activity when compared to immobilised enzyme tube activated by triethyloxonium salt. The optimal condition for direct glutaraldehyde activation of nylon was incubation with 18.5% (w/v) glutaraldehyde in 0.12M borate pH 9.0 for 15 min at 90 °. Springer Science and Business Media LLC 1982 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115073/1/115073.pdf Salleh, Abu Bakar (1982) Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation. Biotechnology Letters, 4 (12). pp. 769-774. ISSN 0141-5492; eISSN: 1573-6776 https://link.springer.com/article/10.1007/BF00131150?error=cookies_not_supported&code=c1b6b9e1-173a-4106-8e9a-b87b964749ea 10.1007/BF00131150 |
| spellingShingle | Salleh, Abu Bakar Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation |
| title | Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation |
| title_full | Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation |
| title_fullStr | Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation |
| title_full_unstemmed | Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation |
| title_short | Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation |
| title_sort | activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation |
| url | http://psasir.upm.edu.my/id/eprint/115073/ http://psasir.upm.edu.my/id/eprint/115073/ http://psasir.upm.edu.my/id/eprint/115073/ http://psasir.upm.edu.my/id/eprint/115073/1/115073.pdf |