Poly(Methyl Methacrylate) as a matrix for immobilization of lipase
Poly(methyl methacrylate) (PMMA) was found to be suitable for the immobilization of lipase from Candida rugosa. The best result based on hydrolytic activity was obtained by adsorption of the purified unbuffered enzyme solution onto PMMA beads without any modification of the beads. Prolonged exposure...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Humana Press
1992
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/114984/ http://psasir.upm.edu.my/id/eprint/114984/1/114984.pdf |
| _version_ | 1848866652838428672 |
|---|---|
| author | Yunus, W. M. Z. Wan Salleh, A. B. Ismail, A. Ampon, K. Razak, C. N. A. Basri, M. |
| author_facet | Yunus, W. M. Z. Wan Salleh, A. B. Ismail, A. Ampon, K. Razak, C. N. A. Basri, M. |
| author_sort | Yunus, W. M. Z. Wan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Poly(methyl methacrylate) (PMMA) was found to be suitable for the immobilization of lipase from Candida rugosa. The best result based on hydrolytic activity was obtained by adsorption of the purified unbuffered enzyme solution onto PMMA beads without any modification of the beads. Prolonged exposure of the protein to the beads increased its adsorption but the expressed activity decreased after 1 h of exposure. The magnitude of the immobilized activity also varied with the size of the beads. Immobilization of the lipase shifted its optimal reaction temperature from 37 to 45°C. The immobilized enzyme is also more stable than the free enzyme in solution. The operational half-life of the immobilized lipase packed in a column and assayed in a closed system is 40 d. |
| first_indexed | 2025-11-15T14:24:01Z |
| format | Article |
| id | upm-114984 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:24:01Z |
| publishDate | 1992 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1149842025-02-13T06:50:08Z http://psasir.upm.edu.my/id/eprint/114984/ Poly(Methyl Methacrylate) as a matrix for immobilization of lipase Yunus, W. M. Z. Wan Salleh, A. B. Ismail, A. Ampon, K. Razak, C. N. A. Basri, M. Poly(methyl methacrylate) (PMMA) was found to be suitable for the immobilization of lipase from Candida rugosa. The best result based on hydrolytic activity was obtained by adsorption of the purified unbuffered enzyme solution onto PMMA beads without any modification of the beads. Prolonged exposure of the protein to the beads increased its adsorption but the expressed activity decreased after 1 h of exposure. The magnitude of the immobilized activity also varied with the size of the beads. Immobilization of the lipase shifted its optimal reaction temperature from 37 to 45°C. The immobilized enzyme is also more stable than the free enzyme in solution. The operational half-life of the immobilized lipase packed in a column and assayed in a closed system is 40 d. Humana Press 1992-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114984/1/114984.pdf Yunus, W. M. Z. Wan and Salleh, A. B. and Ismail, A. and Ampon, K. and Razak, C. N. A. and Basri, M. (1992) Poly(Methyl Methacrylate) as a matrix for immobilization of lipase. Applied Biochemistry and Biotechnology, 36 (2). pp. 97-105. ISSN 1559-0291; eISSN: 0273-2289 https://link.springer.com/article/10.1007/BF02929689?error=cookies_not_supported&code=d0f214c7-9ad4-453d-8977-aa0b0b396090 10.1007/BF02929689 |
| spellingShingle | Yunus, W. M. Z. Wan Salleh, A. B. Ismail, A. Ampon, K. Razak, C. N. A. Basri, M. Poly(Methyl Methacrylate) as a matrix for immobilization of lipase |
| title | Poly(Methyl Methacrylate) as a matrix for immobilization of lipase |
| title_full | Poly(Methyl Methacrylate) as a matrix for immobilization of lipase |
| title_fullStr | Poly(Methyl Methacrylate) as a matrix for immobilization of lipase |
| title_full_unstemmed | Poly(Methyl Methacrylate) as a matrix for immobilization of lipase |
| title_short | Poly(Methyl Methacrylate) as a matrix for immobilization of lipase |
| title_sort | poly(methyl methacrylate) as a matrix for immobilization of lipase |
| url | http://psasir.upm.edu.my/id/eprint/114984/ http://psasir.upm.edu.my/id/eprint/114984/ http://psasir.upm.edu.my/id/eprint/114984/ http://psasir.upm.edu.my/id/eprint/114984/1/114984.pdf |