Effect of pH on the preparation of poly-L-lysine-stabilized calcium alginate beads for immobilization of aminoacylase

The preparations of calcium alginate beads stabilized with poly-L-lysine and encapsulating aminoacylase were conducted at different pH conditions. The interaction of poly-L-lysine and alginate beads proceeds readily. The beads prepared at pH 7.0 exhibited high operational and storage stability with...

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Bibliographic Details
Main Authors: Lee, Kong H., Lee, Pat M.
Format: Article
Language:English
Published: Kluwer Academic Publishers 1993
Online Access:http://psasir.upm.edu.my/id/eprint/114951/
http://psasir.upm.edu.my/id/eprint/114951/1/114951.pdf
Description
Summary:The preparations of calcium alginate beads stabilized with poly-L-lysine and encapsulating aminoacylase were conducted at different pH conditions. The interaction of poly-L-lysine and alginate beads proceeds readily. The beads prepared at pH 7.0 exhibited high operational and storage stability with the elimination of enzyme leakage and the immobilized aminoacylase possessed high biological activity.