Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties
There is an urgent need to explore natural sources like Caulerpa racemosa (Försskal) J. Agardh for bioactive compounds with strong antioxidant and therapeutic potential, providing sustainable alternatives for health and pharmaceutical innovation. This study aimed to determine the phytochemical profi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114854/ http://psasir.upm.edu.my/id/eprint/114854/1/114854.pdf |
| _version_ | 1848866615359176704 |
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| author | Taslim, Nurpudji Astuti Hardinsyah, Hardinsyah Radu, Son Mayulu, Nelly Tsopmo, Apollinaire Kurniawan, Rudy Tallei, Trina Ekawati Herlina, Tati Maksum, Iman Permana Nurkolis, Fahrul |
| author_facet | Taslim, Nurpudji Astuti Hardinsyah, Hardinsyah Radu, Son Mayulu, Nelly Tsopmo, Apollinaire Kurniawan, Rudy Tallei, Trina Ekawati Herlina, Tati Maksum, Iman Permana Nurkolis, Fahrul |
| author_sort | Taslim, Nurpudji Astuti |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | There is an urgent need to explore natural sources like Caulerpa racemosa (Försskal) J. Agardh for bioactive compounds with strong antioxidant and therapeutic potential, providing sustainable alternatives for health and pharmaceutical innovation. This study aimed to determine the phytochemical profile and biological activities of extracts from edible green algae–known as sea grapes (C. racemosa). This present study has successfully identified secondary metabolites through untargeted metabolomic profiling by liquid chromatography-high resolution mass spectrometry (LC-HRMS) as well as a bioactive peptide. The antioxidant activity and cytotoxicity of extracts from C. racemosa and compounds were determined. A total of 103 metabolites were identified in the C. racemosa extract obtained by the maceration (ME), while 48 metabolites were detected in the soxhlet extract (SE). The sequence of the identified peptide was ELWKTF (Glu-Leu-Trp-Lys-Thr-Phe; C41H58N8O) and its abundance was identified in the α-chymotrypsin hydrolysate of C. racemosa. In the antioxidant activity test, SE and purified fraction 1 (PF1) had EC50<EC50 of control or Glutathione (GSH) in terms of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) inhibition, and PF1 had EC50<EC50 of control or Trolox in terms of 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) inhibition. In general, C. racemosa contains antioxidant nutrients, metabolites, and bioactive peptides, suggesting its promising potential as a functional food and pharmaceutical. |
| first_indexed | 2025-11-15T14:23:25Z |
| format | Article |
| id | upm-114854 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:23:25Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1148542025-02-05T03:30:06Z http://psasir.upm.edu.my/id/eprint/114854/ Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties Taslim, Nurpudji Astuti Hardinsyah, Hardinsyah Radu, Son Mayulu, Nelly Tsopmo, Apollinaire Kurniawan, Rudy Tallei, Trina Ekawati Herlina, Tati Maksum, Iman Permana Nurkolis, Fahrul There is an urgent need to explore natural sources like Caulerpa racemosa (Försskal) J. Agardh for bioactive compounds with strong antioxidant and therapeutic potential, providing sustainable alternatives for health and pharmaceutical innovation. This study aimed to determine the phytochemical profile and biological activities of extracts from edible green algae–known as sea grapes (C. racemosa). This present study has successfully identified secondary metabolites through untargeted metabolomic profiling by liquid chromatography-high resolution mass spectrometry (LC-HRMS) as well as a bioactive peptide. The antioxidant activity and cytotoxicity of extracts from C. racemosa and compounds were determined. A total of 103 metabolites were identified in the C. racemosa extract obtained by the maceration (ME), while 48 metabolites were detected in the soxhlet extract (SE). The sequence of the identified peptide was ELWKTF (Glu-Leu-Trp-Lys-Thr-Phe; C41H58N8O) and its abundance was identified in the α-chymotrypsin hydrolysate of C. racemosa. In the antioxidant activity test, SE and purified fraction 1 (PF1) had EC50<EC50 of control or Glutathione (GSH) in terms of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) inhibition, and PF1 had EC50<EC50 of control or Trolox in terms of 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) inhibition. In general, C. racemosa contains antioxidant nutrients, metabolites, and bioactive peptides, suggesting its promising potential as a functional food and pharmaceutical. Elsevier 2024-12 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/114854/1/114854.pdf Taslim, Nurpudji Astuti and Hardinsyah, Hardinsyah and Radu, Son and Mayulu, Nelly and Tsopmo, Apollinaire and Kurniawan, Rudy and Tallei, Trina Ekawati and Herlina, Tati and Maksum, Iman Permana and Nurkolis, Fahrul (2024) Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties. Journal of Agriculture and Food Research, 18. art. no. 101513. pp. 1-15. ISSN 2666-1543; eISSN: 2666-1543 https://linkinghub.elsevier.com/retrieve/pii/S2666154324005507 10.1016/j.jafr.2024.101513 |
| spellingShingle | Taslim, Nurpudji Astuti Hardinsyah, Hardinsyah Radu, Son Mayulu, Nelly Tsopmo, Apollinaire Kurniawan, Rudy Tallei, Trina Ekawati Herlina, Tati Maksum, Iman Permana Nurkolis, Fahrul Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties |
| title | Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties |
| title_full | Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties |
| title_fullStr | Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties |
| title_full_unstemmed | Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties |
| title_short | Functional food candidate from Indonesian green algae Caulerpa racemosa (Försskal) J. Agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties |
| title_sort | functional food candidate from indonesian green algae caulerpa racemosa (försskal) j. agardh by two extraction methods: metabolite profile, antioxidant activity, and cytotoxic properties |
| url | http://psasir.upm.edu.my/id/eprint/114854/ http://psasir.upm.edu.my/id/eprint/114854/ http://psasir.upm.edu.my/id/eprint/114854/ http://psasir.upm.edu.my/id/eprint/114854/1/114854.pdf |