The characterisation of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae in a teaching hospital in Malaysia
Background/Objectives: The emergence and dissemination of carbapenem-resistant organisms, particularly Acinetobacter baumannii and Klebsiella pneumoniae, pose a significant threat to healthcare systems worldwide. This retrospective study aims to characterise carbapenem-resistant Acinetobacter bauman...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute (MDPI)
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114803/ http://psasir.upm.edu.my/id/eprint/114803/1/114803.pdf |
| _version_ | 1848866599895826432 |
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| author | Lau, Min Yi Ponnampalavanar, Sasheela Chong, Chun Wie Dwiyanto, Jacky Lee, Yee Qing Woon, Jia Jie Kong, Zhi Xian Jasni, Azmiza Syawani Lee, Michelle Chin Chin Obaidellah, Unaizah Hanum Teh, Cindy Shuan Ju |
| author_facet | Lau, Min Yi Ponnampalavanar, Sasheela Chong, Chun Wie Dwiyanto, Jacky Lee, Yee Qing Woon, Jia Jie Kong, Zhi Xian Jasni, Azmiza Syawani Lee, Michelle Chin Chin Obaidellah, Unaizah Hanum Teh, Cindy Shuan Ju |
| author_sort | Lau, Min Yi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background/Objectives: The emergence and dissemination of carbapenem-resistant organisms, particularly Acinetobacter baumannii and Klebsiella pneumoniae, pose a significant threat to healthcare systems worldwide. This retrospective study aims to characterise carbapenem-resistant Acinetobacter baumannii (CRAB) and carbapenem-resistant Klebsiella pneumoniae (CRKP) strains in a teaching hospital and to determine the risk factors associated with patients’ in-hospital mortality. Methods: A total of 90 CRAB and 63 CRKP were included in this study. Carbapenemase genes and MLST types of CRAB and CRKP were determined using specific primers. Risk factors associated with in-hospital mortality were analysed with collected data. Results: All the CRAB strains consisted of OXA carbapenemase genes, with 98% of the strains co-harbouring blaOXA-23-like and blaOXA-51-like carbapenemase genes. Conversely, blaNDM is the predominant carbapenemase gene in CRKP, followed by blaOXA-48-like carbapenemase genes. ST2 and ST20 are the dominant MLST types in CRAB and CRKP, respectively. In CRAB, multivariate analysis identified age, ethnicity, the presence of a mechanical ventilator, and patients who experienced previous exposure to clindamycin in the last 90 days as associated with an increased risk of in-hospital mortality. In contrast, older age, male, ICU admission, and the presence of an indwelling urinary catheter were significantly associated with an increased risk of mortality for patients with CRKP. Conclusions: Both CRAB and CRKP lead to high rates of mortality. The MLST profile showed that the genomic patterns of CRKP were highly diverse, whereas CRAB strains had low genetic diversity. To tackle these challenging pathogens, robust surveillance and an in-depth understanding of molecular epidemiology and genomics studies are needed to tailor infection control strategies and individualise treatment approaches. |
| first_indexed | 2025-11-15T14:23:10Z |
| format | Article |
| id | upm-114803 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:23:10Z |
| publishDate | 2024 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1148032025-02-03T00:54:41Z http://psasir.upm.edu.my/id/eprint/114803/ The characterisation of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae in a teaching hospital in Malaysia Lau, Min Yi Ponnampalavanar, Sasheela Chong, Chun Wie Dwiyanto, Jacky Lee, Yee Qing Woon, Jia Jie Kong, Zhi Xian Jasni, Azmiza Syawani Lee, Michelle Chin Chin Obaidellah, Unaizah Hanum Teh, Cindy Shuan Ju Background/Objectives: The emergence and dissemination of carbapenem-resistant organisms, particularly Acinetobacter baumannii and Klebsiella pneumoniae, pose a significant threat to healthcare systems worldwide. This retrospective study aims to characterise carbapenem-resistant Acinetobacter baumannii (CRAB) and carbapenem-resistant Klebsiella pneumoniae (CRKP) strains in a teaching hospital and to determine the risk factors associated with patients’ in-hospital mortality. Methods: A total of 90 CRAB and 63 CRKP were included in this study. Carbapenemase genes and MLST types of CRAB and CRKP were determined using specific primers. Risk factors associated with in-hospital mortality were analysed with collected data. Results: All the CRAB strains consisted of OXA carbapenemase genes, with 98% of the strains co-harbouring blaOXA-23-like and blaOXA-51-like carbapenemase genes. Conversely, blaNDM is the predominant carbapenemase gene in CRKP, followed by blaOXA-48-like carbapenemase genes. ST2 and ST20 are the dominant MLST types in CRAB and CRKP, respectively. In CRAB, multivariate analysis identified age, ethnicity, the presence of a mechanical ventilator, and patients who experienced previous exposure to clindamycin in the last 90 days as associated with an increased risk of in-hospital mortality. In contrast, older age, male, ICU admission, and the presence of an indwelling urinary catheter were significantly associated with an increased risk of mortality for patients with CRKP. Conclusions: Both CRAB and CRKP lead to high rates of mortality. The MLST profile showed that the genomic patterns of CRKP were highly diverse, whereas CRAB strains had low genetic diversity. To tackle these challenging pathogens, robust surveillance and an in-depth understanding of molecular epidemiology and genomics studies are needed to tailor infection control strategies and individualise treatment approaches. Multidisciplinary Digital Publishing Institute (MDPI) 2024-11-20 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/114803/1/114803.pdf Lau, Min Yi and Ponnampalavanar, Sasheela and Chong, Chun Wie and Dwiyanto, Jacky and Lee, Yee Qing and Woon, Jia Jie and Kong, Zhi Xian and Jasni, Azmiza Syawani and Lee, Michelle Chin Chin and Obaidellah, Unaizah Hanum and Teh, Cindy Shuan Ju (2024) The characterisation of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae in a teaching hospital in Malaysia. Antibiotics, 13 (11). art. no. 1107. ISSN 2079-6382; eISSN: 2079-6382 https://www.mdpi.com/2079-6382/13/11/1107 10.3390/antibiotics13111107 |
| spellingShingle | Lau, Min Yi Ponnampalavanar, Sasheela Chong, Chun Wie Dwiyanto, Jacky Lee, Yee Qing Woon, Jia Jie Kong, Zhi Xian Jasni, Azmiza Syawani Lee, Michelle Chin Chin Obaidellah, Unaizah Hanum Teh, Cindy Shuan Ju The characterisation of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae in a teaching hospital in Malaysia |
| title | The characterisation of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae in a teaching hospital in Malaysia |
| title_full | The characterisation of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae in a teaching hospital in Malaysia |
| title_fullStr | The characterisation of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae in a teaching hospital in Malaysia |
| title_full_unstemmed | The characterisation of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae in a teaching hospital in Malaysia |
| title_short | The characterisation of carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae in a teaching hospital in Malaysia |
| title_sort | characterisation of carbapenem-resistant acinetobacter baumannii and klebsiella pneumoniae in a teaching hospital in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/114803/ http://psasir.upm.edu.my/id/eprint/114803/ http://psasir.upm.edu.my/id/eprint/114803/ http://psasir.upm.edu.my/id/eprint/114803/1/114803.pdf |