Sugar esterification catalysed by alkylated trypsin in dimethylformamide
Reductive alkylation of porcine pancreatic trypsin with acetaldehyde, propionaldehyde, octaldehyde and benzaldehyde resulted in about 5 to 6 folds increase in the sugar esterification activities of the enzyme in DMF. The optimum activities of the modified enzymes depend on the degrees of their modif...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Kluwer Academic Publishers
1991
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114788/ http://psasir.upm.edu.my/id/eprint/114788/1/114788.pdf |
| _version_ | 1848866595529555968 |
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| author | Ampon, K. Salleh, A.B. Teoh, A. Wan Yunus, W.M.Z. |
| author_facet | Ampon, K. Salleh, A.B. Teoh, A. Wan Yunus, W.M.Z. |
| author_sort | Ampon, K. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Reductive alkylation of porcine pancreatic trypsin with acetaldehyde, propionaldehyde, octaldehyde and benzaldehyde resulted in about 5 to 6 folds increase in the sugar esterification activities of the enzyme in DMF. The optimum activities of the modified enzymes depend on the degrees of their modification with the respective aldehydes. These alkylated trypsins were more stable in DMF compared to the native unmodified enzyme at temperatures between 26 and 60°C. © 1991 Kluwer Academic Publishers. |
| first_indexed | 2025-11-15T14:23:06Z |
| format | Article |
| id | upm-114788 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:23:06Z |
| publishDate | 1991 |
| publisher | Kluwer Academic Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1147882025-01-31T03:58:53Z http://psasir.upm.edu.my/id/eprint/114788/ Sugar esterification catalysed by alkylated trypsin in dimethylformamide Ampon, K. Salleh, A.B. Teoh, A. Wan Yunus, W.M.Z. Reductive alkylation of porcine pancreatic trypsin with acetaldehyde, propionaldehyde, octaldehyde and benzaldehyde resulted in about 5 to 6 folds increase in the sugar esterification activities of the enzyme in DMF. The optimum activities of the modified enzymes depend on the degrees of their modification with the respective aldehydes. These alkylated trypsins were more stable in DMF compared to the native unmodified enzyme at temperatures between 26 and 60°C. © 1991 Kluwer Academic Publishers. Kluwer Academic Publishers 1991 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114788/1/114788.pdf Ampon, K. and Salleh, A.B. and Teoh, A. and Wan Yunus, W.M.Z. (1991) Sugar esterification catalysed by alkylated trypsin in dimethylformamide. Biotechnology Letters, 13 (1). pp. 25-30. ISSN 1573-6776; eISSN: 0141-5492 https://link.springer.com/article/10.1007/BF01033511?error=cookies_not_supported&code=d314132a-177e-48a4-8f48-a76a7bc74246 10.1007/BF01033511 |
| spellingShingle | Ampon, K. Salleh, A.B. Teoh, A. Wan Yunus, W.M.Z. Sugar esterification catalysed by alkylated trypsin in dimethylformamide |
| title | Sugar esterification catalysed by alkylated trypsin in dimethylformamide |
| title_full | Sugar esterification catalysed by alkylated trypsin in dimethylformamide |
| title_fullStr | Sugar esterification catalysed by alkylated trypsin in dimethylformamide |
| title_full_unstemmed | Sugar esterification catalysed by alkylated trypsin in dimethylformamide |
| title_short | Sugar esterification catalysed by alkylated trypsin in dimethylformamide |
| title_sort | sugar esterification catalysed by alkylated trypsin in dimethylformamide |
| url | http://psasir.upm.edu.my/id/eprint/114788/ http://psasir.upm.edu.my/id/eprint/114788/ http://psasir.upm.edu.my/id/eprint/114788/ http://psasir.upm.edu.my/id/eprint/114788/1/114788.pdf |