Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells
Objective: Concerns over the increasing number of obese individuals and the associated health risks have prompted therapeutic option explorations. Similarly, this study aimed to establish Sygyzium claviflorum fruit extract (SCFE) anti-adipogenic attributes in 3T3-L1 cells. Methods: The polyphenolic...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier B.V.
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/114761/ http://psasir.upm.edu.my/id/eprint/114761/1/114761.pdf |
| _version_ | 1848866587728150528 |
|---|---|
| author | Zohdi, Rozaini M. Adli, Muhammad A. Mukhtar, Shahida M. Awang Junaidi, Awang H. Bakar, Md Zuki A. |
| author_facet | Zohdi, Rozaini M. Adli, Muhammad A. Mukhtar, Shahida M. Awang Junaidi, Awang H. Bakar, Md Zuki A. |
| author_sort | Zohdi, Rozaini M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: Concerns over the increasing number of obese individuals and the associated health risks have prompted therapeutic option explorations. Similarly, this study aimed to establish Sygyzium claviflorum fruit extract (SCFE) anti-adipogenic attributes in 3T3-L1 cells. Methods: The polyphenolic compounds in SCFE were identified with Reverse phase-high performance liquid chromatography (RP-HPLC). Meanwhile, murine 3T3-L1 preadipocytes, measuring leptin levels, reactive oxygen species (ROS), and lipid and triglyceride (TG) contents were utilized during anti-adipogenic activity assessments. Concurrently, the effects of SCFE on adipogenic transcription factors were established with quantitative real-time-polymerase chain reaction (qRT-PCR). Results: The RP-HPLC results indicated three polyphenolic compounds in SCFE, including one flavonoid (naringin) and two phenolic acids (syringic and p-coumaric). Although SCFE treatments (250–1000 μg/mL) did not result in cell toxicity, they significantly reduced dose-dependent lipid accumulation, ROS production, and TG and leptin levels relative to control-differentiated adipocytes. Moreover, SCFE suppressed sterol regulatory element binding protein-1 (SREBP-1), peroxisome proliferator-activated receptor-gamma (PPAR-γ), and CCAAT/enhancer-binding protein-alpha (C/EBP-α) gene expressions during preadipocyte differentiation into adipocytes. Conclusion: The findings revealed the anti-adipogenic properties of SCFE, indicating its potential as a natural obesity management remedy. Nevertheless, more studies are necessary to elucidate the reactions resulting in SCFE anti-adipogenic effects and the active constituents responsible for the property. |
| first_indexed | 2025-11-15T14:22:59Z |
| format | Article |
| id | upm-114761 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:22:59Z |
| publishDate | 2024 |
| publisher | Elsevier B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1147612025-01-31T01:02:14Z http://psasir.upm.edu.my/id/eprint/114761/ Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells Zohdi, Rozaini M. Adli, Muhammad A. Mukhtar, Shahida M. Awang Junaidi, Awang H. Bakar, Md Zuki A. Objective: Concerns over the increasing number of obese individuals and the associated health risks have prompted therapeutic option explorations. Similarly, this study aimed to establish Sygyzium claviflorum fruit extract (SCFE) anti-adipogenic attributes in 3T3-L1 cells. Methods: The polyphenolic compounds in SCFE were identified with Reverse phase-high performance liquid chromatography (RP-HPLC). Meanwhile, murine 3T3-L1 preadipocytes, measuring leptin levels, reactive oxygen species (ROS), and lipid and triglyceride (TG) contents were utilized during anti-adipogenic activity assessments. Concurrently, the effects of SCFE on adipogenic transcription factors were established with quantitative real-time-polymerase chain reaction (qRT-PCR). Results: The RP-HPLC results indicated three polyphenolic compounds in SCFE, including one flavonoid (naringin) and two phenolic acids (syringic and p-coumaric). Although SCFE treatments (250–1000 μg/mL) did not result in cell toxicity, they significantly reduced dose-dependent lipid accumulation, ROS production, and TG and leptin levels relative to control-differentiated adipocytes. Moreover, SCFE suppressed sterol regulatory element binding protein-1 (SREBP-1), peroxisome proliferator-activated receptor-gamma (PPAR-γ), and CCAAT/enhancer-binding protein-alpha (C/EBP-α) gene expressions during preadipocyte differentiation into adipocytes. Conclusion: The findings revealed the anti-adipogenic properties of SCFE, indicating its potential as a natural obesity management remedy. Nevertheless, more studies are necessary to elucidate the reactions resulting in SCFE anti-adipogenic effects and the active constituents responsible for the property. Elsevier B.V. 2024 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/114761/1/114761.pdf Zohdi, Rozaini M. and Adli, Muhammad A. and Mukhtar, Shahida M. and Awang Junaidi, Awang H. and Bakar, Md Zuki A. (2024) Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells. Journal of Taibah University Medical Sciences, 19 (6). pp. 1181-1192. ISSN 1658-3612; eISSN: 1658-3612 https://linkinghub.elsevier.com/retrieve/pii/S1658361224001525 10.1016/j.jtumed.2024.12.008 |
| spellingShingle | Zohdi, Rozaini M. Adli, Muhammad A. Mukhtar, Shahida M. Awang Junaidi, Awang H. Bakar, Md Zuki A. Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells |
| title | Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells |
| title_full | Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells |
| title_fullStr | Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells |
| title_full_unstemmed | Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells |
| title_short | Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells |
| title_sort | sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3t3-l1 cells |
| url | http://psasir.upm.edu.my/id/eprint/114761/ http://psasir.upm.edu.my/id/eprint/114761/ http://psasir.upm.edu.my/id/eprint/114761/ http://psasir.upm.edu.my/id/eprint/114761/1/114761.pdf |