Distribution of an enzyme in porous polymer beads
Trypsin has been immobilized by adsorption onto Amberlite XAD‐7 beads. The Michaelis constant (Km) of the enzyme was increased about sevenfold following the immobilization. Its rate of penetration into the porous beads was determined by staining the beads, which had been split, with naphthol blue bl...
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| Format: | Article |
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1992
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114746/ |
| _version_ | 1848866583347200000 |
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| author | Ampon, Kamaruzaman |
| author_facet | Ampon, Kamaruzaman |
| author_sort | Ampon, Kamaruzaman |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Trypsin has been immobilized by adsorption onto Amberlite XAD‐7 beads. The Michaelis constant (Km) of the enzyme was increased about sevenfold following the immobilization. Its rate of penetration into the porous beads was determined by staining the beads, which had been split, with naphthol blue black. The extent of diffusional rate limitation of immobilized trypsin was related to the penetration depth of the enzyme into the beads. This can be controlled by manipulating the conditions during the preparation of the immobilized enzyme. Copyright © 1992 Society of Chemical Industry |
| first_indexed | 2025-11-15T14:22:54Z |
| format | Article |
| id | upm-114746 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:22:54Z |
| publishDate | 1992 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1147462025-01-28T06:55:57Z http://psasir.upm.edu.my/id/eprint/114746/ Distribution of an enzyme in porous polymer beads Ampon, Kamaruzaman Trypsin has been immobilized by adsorption onto Amberlite XAD‐7 beads. The Michaelis constant (Km) of the enzyme was increased about sevenfold following the immobilization. Its rate of penetration into the porous beads was determined by staining the beads, which had been split, with naphthol blue black. The extent of diffusional rate limitation of immobilized trypsin was related to the penetration depth of the enzyme into the beads. This can be controlled by manipulating the conditions during the preparation of the immobilized enzyme. Copyright © 1992 Society of Chemical Industry 1992 Article PeerReviewed Ampon, Kamaruzaman (1992) Distribution of an enzyme in porous polymer beads. Journal of Chemical Technology & Biotechnology, 55 (2). pp. 185-190. ISSN 0268-2575; eISSN: 1097-4660 https://onlinelibrary.wiley.com/doi/10.1002/jctb.280550213 10.1002/jctb.280550213 |
| spellingShingle | Ampon, Kamaruzaman Distribution of an enzyme in porous polymer beads |
| title | Distribution of an enzyme in porous polymer beads |
| title_full | Distribution of an enzyme in porous polymer beads |
| title_fullStr | Distribution of an enzyme in porous polymer beads |
| title_full_unstemmed | Distribution of an enzyme in porous polymer beads |
| title_short | Distribution of an enzyme in porous polymer beads |
| title_sort | distribution of an enzyme in porous polymer beads |
| url | http://psasir.upm.edu.my/id/eprint/114746/ http://psasir.upm.edu.my/id/eprint/114746/ http://psasir.upm.edu.my/id/eprint/114746/ |