Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits
Purpose: To determine the effect of aqueous extract of Channa striatus on plasma lipids concentrations, pro-inflammatory cytokines levels, adhesion molecules and arterial plaque formation in cholesterol-fed rabbits. Methods: New Zealand rabbits (n = 30, females) were divided into five groups of six...
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| Format: | Article |
| Language: | English |
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University of Benin
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114718/ http://psasir.upm.edu.my/id/eprint/114718/1/114718.pdf |
| _version_ | 1848866575182987264 |
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| author | Hakim, Muhammad Nazrul Yee, Pang Chong Cheema, Manraj Singh Keong, Yong Yoke Ahmad, Zuraini |
| author_facet | Hakim, Muhammad Nazrul Yee, Pang Chong Cheema, Manraj Singh Keong, Yong Yoke Ahmad, Zuraini |
| author_sort | Hakim, Muhammad Nazrul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Purpose: To determine the effect of aqueous extract of Channa striatus on plasma lipids concentrations, pro-inflammatory cytokines levels, adhesion molecules and arterial plaque formation in cholesterol-fed rabbits. Methods: New Zealand rabbits (n = 30, females) were divided into five groups of six rabbits each as follows: cholesterol control group (0.5 % cholesterol); normal control group (normal diet), positive control group (0.5 % cholesterol + 5 mg/kg atorvastatin); and treatment groups (0.5% cholesterol + 250 mg/kg or 500 mg/kg C. striatus extracts, respectively) for 6 weeks. Blood was taken every 2 weeks and analyzed for total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglyceride (TG), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). Atherogenic index (AI) was calculated based on LDL:HDL ratio. Results: Cholesterol feeding for 6 weeks resulted in significantly increased TC, LDL, HDL, AI, TG, TNF-α and IL-1β when compared to C. striatus-fed rabbits. Serum LDL was 26.34 ± 1.43 mmol/L in C. striatus group to 30.52 ± 0.82 mmol/L in cholesterol group (p < 0.05). C. striatus extract (250 mg/kg) increased HDL by approximately 400 % (6.23 ± 1.76 mmol/L from 0.72 ± 0.18 mmol/L), decreased AI value (4.6 in C. striatus group to 10.4 in cholesterol group). Also, IL-1β and TNF-α concentration also significantly (p < 0.05) reduced with C. striatus administration. Interestingly, down-regulation of ICAM-1 and VCAM-1 expression and reduced plaque formation were observed with C. striatus feeding. Conclusion: Channa striatus extract is effective in reducing atherogenesis. This could be due to the high HDL level produced in 250 mg/kg dose group, and strongly suggests the significance of HDL in preventing atherogenesis even if other lipid profiles remain unchanged. |
| first_indexed | 2025-11-15T14:22:47Z |
| format | Article |
| id | upm-114718 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:22:47Z |
| publishDate | 2024 |
| publisher | University of Benin |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1147182025-01-24T04:11:21Z http://psasir.upm.edu.my/id/eprint/114718/ Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits Hakim, Muhammad Nazrul Yee, Pang Chong Cheema, Manraj Singh Keong, Yong Yoke Ahmad, Zuraini Purpose: To determine the effect of aqueous extract of Channa striatus on plasma lipids concentrations, pro-inflammatory cytokines levels, adhesion molecules and arterial plaque formation in cholesterol-fed rabbits. Methods: New Zealand rabbits (n = 30, females) were divided into five groups of six rabbits each as follows: cholesterol control group (0.5 % cholesterol); normal control group (normal diet), positive control group (0.5 % cholesterol + 5 mg/kg atorvastatin); and treatment groups (0.5% cholesterol + 250 mg/kg or 500 mg/kg C. striatus extracts, respectively) for 6 weeks. Blood was taken every 2 weeks and analyzed for total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglyceride (TG), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). Atherogenic index (AI) was calculated based on LDL:HDL ratio. Results: Cholesterol feeding for 6 weeks resulted in significantly increased TC, LDL, HDL, AI, TG, TNF-α and IL-1β when compared to C. striatus-fed rabbits. Serum LDL was 26.34 ± 1.43 mmol/L in C. striatus group to 30.52 ± 0.82 mmol/L in cholesterol group (p < 0.05). C. striatus extract (250 mg/kg) increased HDL by approximately 400 % (6.23 ± 1.76 mmol/L from 0.72 ± 0.18 mmol/L), decreased AI value (4.6 in C. striatus group to 10.4 in cholesterol group). Also, IL-1β and TNF-α concentration also significantly (p < 0.05) reduced with C. striatus administration. Interestingly, down-regulation of ICAM-1 and VCAM-1 expression and reduced plaque formation were observed with C. striatus feeding. Conclusion: Channa striatus extract is effective in reducing atherogenesis. This could be due to the high HDL level produced in 250 mg/kg dose group, and strongly suggests the significance of HDL in preventing atherogenesis even if other lipid profiles remain unchanged. University of Benin 2024-09-06 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/114718/1/114718.pdf Hakim, Muhammad Nazrul and Yee, Pang Chong and Cheema, Manraj Singh and Keong, Yong Yoke and Ahmad, Zuraini (2024) Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits. Tropical Journal of Pharmaceutical Research, 23 (7). pp. 1083-1092. ISSN 1596-5996; eISSN: 1596-9827 https://www.ajol.info/index.php/tjpr/article/view/278008 10.4314/tjpr.v23i7.6 |
| spellingShingle | Hakim, Muhammad Nazrul Yee, Pang Chong Cheema, Manraj Singh Keong, Yong Yoke Ahmad, Zuraini Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits |
| title | Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits |
| title_full | Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits |
| title_fullStr | Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits |
| title_full_unstemmed | Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits |
| title_short | Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits |
| title_sort | anti-atherogenic effect of channa striatus fish extract in high cholesterol-fed rabbits |
| url | http://psasir.upm.edu.my/id/eprint/114718/ http://psasir.upm.edu.my/id/eprint/114718/ http://psasir.upm.edu.my/id/eprint/114718/ http://psasir.upm.edu.my/id/eprint/114718/1/114718.pdf |