Development of a recombinant non-replicating Newcastle disease virus
Newcastle disease virus (NDV) is an avian orthoavulavirus I that causes high morbidity and mortality in birds, depending on the pathotype of the virus. Several clinical studies have shown that NDV is a promising oncolytic virus for cancer therapies, yet its application to clinics is restricted due t...
| Main Authors: | , , , , |
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| Format: | Article |
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Taylor and Francis
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114712/ |
| _version_ | 1848866573460176896 |
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| author | Cheow, Pheik-Sheen Tan, Tiong Kit Song, Adelene Ai-Lian Yusoff, Khatijah Chia, Suet Lin |
| author_facet | Cheow, Pheik-Sheen Tan, Tiong Kit Song, Adelene Ai-Lian Yusoff, Khatijah Chia, Suet Lin |
| author_sort | Cheow, Pheik-Sheen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Newcastle disease virus (NDV) is an avian orthoavulavirus I that causes high morbidity and mortality in birds, depending on the pathotype of the virus. Several clinical studies have shown that NDV is a promising oncolytic virus for cancer therapies, yet its application to clinics is restricted due to the pathogenicity of this virus in poultry. Hence, it is important to produce a recombinant NDV (rNDV) with reduced virulence to make it a fit candidate as an oncolytic virus. In this study, we aimed to produce a rNDV with a truncated ant-igenome via reverse genetics to render it non-replicating (nr). The genes responsible for replication were removed from the NDV genome. The rescued nr-NDV was verified, propagated, and purified from tissue culture medium. The virus failed to propagate in embryonated eggs, confirming its non-replicating property. The virus retained its oncolytic activities similar to that of the replicating rAF-GFP as observed in a cell viability assay when a breast cancer cell line, MCF-7, was treated with the virus. In conclusion, this study has successfully generated a system to produce a non-replicating NDV which can be used as a viral vector for heterologous protein delivery, as a safer alternative compared to the virulent strains as an oncolytic virus. RESEARCH HIGHLIGHTS: Development of nr-NDV. Reverse transfection was applied for the recovery of nr-NDV. Propagation of nr-NDV was done by sub-passaging transfected BSR T7/5 cells. Safety profile was done to prove that the nr-NDV is non-replicating. |
| first_indexed | 2025-11-15T14:22:45Z |
| format | Article |
| id | upm-114712 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:22:45Z |
| publishDate | 2024 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1147122025-01-24T03:02:18Z http://psasir.upm.edu.my/id/eprint/114712/ Development of a recombinant non-replicating Newcastle disease virus Cheow, Pheik-Sheen Tan, Tiong Kit Song, Adelene Ai-Lian Yusoff, Khatijah Chia, Suet Lin Newcastle disease virus (NDV) is an avian orthoavulavirus I that causes high morbidity and mortality in birds, depending on the pathotype of the virus. Several clinical studies have shown that NDV is a promising oncolytic virus for cancer therapies, yet its application to clinics is restricted due to the pathogenicity of this virus in poultry. Hence, it is important to produce a recombinant NDV (rNDV) with reduced virulence to make it a fit candidate as an oncolytic virus. In this study, we aimed to produce a rNDV with a truncated ant-igenome via reverse genetics to render it non-replicating (nr). The genes responsible for replication were removed from the NDV genome. The rescued nr-NDV was verified, propagated, and purified from tissue culture medium. The virus failed to propagate in embryonated eggs, confirming its non-replicating property. The virus retained its oncolytic activities similar to that of the replicating rAF-GFP as observed in a cell viability assay when a breast cancer cell line, MCF-7, was treated with the virus. In conclusion, this study has successfully generated a system to produce a non-replicating NDV which can be used as a viral vector for heterologous protein delivery, as a safer alternative compared to the virulent strains as an oncolytic virus. RESEARCH HIGHLIGHTS: Development of nr-NDV. Reverse transfection was applied for the recovery of nr-NDV. Propagation of nr-NDV was done by sub-passaging transfected BSR T7/5 cells. Safety profile was done to prove that the nr-NDV is non-replicating. Taylor and Francis 2024-09-25 Article PeerReviewed Cheow, Pheik-Sheen and Tan, Tiong Kit and Song, Adelene Ai-Lian and Yusoff, Khatijah and Chia, Suet Lin (2024) Development of a recombinant non-replicating Newcastle disease virus. Avian Pathology, 54 (1). pp. 149-157. ISSN 0307-9457; eISSN: 1465-3338 https://www.tandfonline.com/doi/full/10.1080/03079457.2024.2403412 10.1080/03079457.2024.2403412 |
| spellingShingle | Cheow, Pheik-Sheen Tan, Tiong Kit Song, Adelene Ai-Lian Yusoff, Khatijah Chia, Suet Lin Development of a recombinant non-replicating Newcastle disease virus |
| title | Development of a recombinant non-replicating Newcastle disease virus |
| title_full | Development of a recombinant non-replicating Newcastle disease virus |
| title_fullStr | Development of a recombinant non-replicating Newcastle disease virus |
| title_full_unstemmed | Development of a recombinant non-replicating Newcastle disease virus |
| title_short | Development of a recombinant non-replicating Newcastle disease virus |
| title_sort | development of a recombinant non-replicating newcastle disease virus |
| url | http://psasir.upm.edu.my/id/eprint/114712/ http://psasir.upm.edu.my/id/eprint/114712/ http://psasir.upm.edu.my/id/eprint/114712/ |