Homology modeling and expression of recombinant NS5-RDRP based on the Indonesian local strain of dengue virus for anti-dengue drug development

QR Code

Homology modeling and expression of recombinant NS5-RDRP based on the Indonesian local strain of dengue virus for anti-dengue drug development

This study aims to isolate the RNA-dependent RNA polymerase (RdRp) gene of the dengue virus (DENV) isolates from Indonesia, determine the serotype of the local DENV strain through homology modeling, and express the recombinant RdRp protein in Escherichia coli BL21 (DE3), as the target for anti-dengu...

Full description

Bibliographic Details
Main Authors:	Subroto, Toto, Firdaus, Ade Rizqi Ridwan, Kusumawardani, Shinta, Tohari, Taufik Ramdani, Nababan, Theresia Adventina, Pertiwi, Wulan, Fadhlillah, Muhammad, Kusuma, Sri Agung Fitri, Rukayadi, Yaya
Format:	Article
Language:	English
Published:	HH Publisher 2024
Online Access:	http://psasir.upm.edu.my/id/eprint/114680/ http://psasir.upm.edu.my/id/eprint/114680/1/114680.pdf

Similar Items

Computational Design And Synthesis Of Potential Ns2b-Ns3 Dengue Protease Inhibitors
by: Salin, Nurul Hanim
Published: (2024)

Ligand based drug discovery of novel dengue-2 NS2B-NS3 protease inhibitors
by: Mohd Nawi, Mohamed Sufian, et al.
Published: (2016)

In-silico design of curcumin analogs as potential inhibitors of dengue virus NS2B/NS3 protease
by: Roney, Miah, et al.
Published: (2023)

Ligand Based Drug Discovery Of Novel Dengue-2 NS2B-NS3 Protease Inhibitors
by: Mohd Nawi, Mohamed Sufian
Published: (2015)

Structure Based Design And Synthesis Of Potential Dengue Virus NS2B-NS3 Protease Inhibitors
by: Sinha,, Suman
Published: (2018)

Dengue virus Non-structural (NS) 1 gene as a molecular marker for early detection of in vitro dengue virus infection
by: Nur Azizah A Rahman,, et al.
Published: (2021)

Prevalence of NS1 Dengue Antigen among Blood Donors In Perak and Penang
by: Mohamed Yusuf, Mohd Faizal
Published: (2016)

Virtual Screening And In Vitro Assay Of Potential Inhibitors Against Dengue-2 NS2B-NS3 Protease
by: Ganesh, Vanee
Published: (2016)

Development And Application Of Dengue Virus Ns2b/Ns3 Protease Inhibition Assay Using Alphascreen® Technology.
by: Abduraman, Muhammad Asyraf
Published: (2015)

In Vitro And In Silico Studies On Selected Malaysia Culinary Plants Against Dengue Ns2b-Ns3 Protease
by: Yong , Kai Sing
Published: (2016)

An electrochemical immunobiosensor for dengue virus NS1 protein detection / Nadiya Taha Darwish
by: Nadiya Taha, Darwish
Published: (2016)

Generation And Characterization Of Rna Aptamer Against Dengue Virus 2 Ns1 Glycoprotein
by: Sivalingam, Rogini
Published: (2018)

Improving of pelB-Secreted MPT64 protein released by Escherichia coli BL21 (DE3) using Triton X-100 and Tween-80
by: Kusuma, Sri Agung Fitri, et al.
Published: (2022)

Molecular docking and drug-likeness study of nirmatrelvir as promising drug candidates of dengue virus NS2B-NS3 protease
by: Huq, A.K.M. Moyeenul, et al.
Published: (2023)

Rational Design And Synthesis Of Peptide Inhibitors Of Ns2b/Ns3 Protease As Therapeutic Agents For Dengue Virus Type 2
by: Abdalrazaq, Nadeem Abdalsatar
Published: (2021)

Computational evaluation of quinones of Nigella sativa L. as potential inhibitor of dengue virus NS5
by: Roney, Miah, et al.
Published: (2023)

Development Of An Enzyme-Linked Aptasorbant Assay In Detecting Dengue Virus 2 Ns1 Biomarker
by: Ramesh, Thevendran
Published: (2022)

Biological activities of quercus infectoria aqueous extract toward dengue-2 NS2B/NS3 protease / Ammar Y. Abdulrahman
by: Abdulrahman, Ammar Y.
Published: (2013)

Expression Of Recombinant Denv Ns2b/Ns3 Protease: In Silico Screening And In Vitro Validation Of Novel Antiviral Compounds For Dengue Virus Inhibition
by: Harun, Norshidah
Published: (2024)

Recombinant Ns3 Serine Protease From Dengue Virus 2 As A Screen For Small Molecules
by: Ab Aziz, Nurohaida
Published: (2011)

Recombinant Ns3 Serine Protease From Dengue Virus 2 As A Screen For Small Molecules
by: Ab Aziz, Nurohaida
Published: (2011)

Phenolic compounds of Theobroma cacao L. show potential against dengue RdRp protease enzyme inhibition by In-silico docking, DFT study, MD simulation and MMGBSA calculation
by: Moyeenul Huq, A. K. M., et al.
Published: (2024)

Dengue fever presenting as bilateral dengue maculopathy
by: Umi Kalthum, MN, et al.
Published: (2012)

Insight into the in silico study and biological evaluation of curcumin analogue compounds as new potential inhibitors for dengue DEN2 NS2B/NS3 serine protease
by: Frimayanti, Neni, et al.
Published: (2024)

Purification of anti-Mycobacterium tuberculosis MPT64 immunoglobulin-Y from egg-yolk supernatant using thiophilic adsorption chromatography
by: Kusuma, Sri Agung Fitri, et al.
Published: (2024)

Salivary based sers analysis in recognition of ns1 for pcasvm classification of dengue fever / Afaf Rozan Mohd Radzol
by: Mohd Radzol, Afaf Rozan
Published: (2018)

Improvement of extracellular secretion efficiency of recombinant protein from Escherichia coli: signal peptide fusion, surfactants addition, and phospholipase
by: Kusuma, Sri Agung Fitri, et al.
Published: (2019)

Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology
by: Kusuma, Sri Agung Fitri, et al.
Published: (2019)

Salivary based SERS analysia in recognition of NS1 for PCA-SVM classification of dengue fever / Afaf Rozan Mohd Radzol
by: Mohd Radzol, Afaf Rozan
Published: (2018)

Development Of Dengue Immunodiagnostic Assay By Targeting Denv Type 2 Ns1 Using Shark Single Domain Vnar Antibody
by: Kok, Boon Hui
Published: (2024)

Development of dengue vaccines
by: Lim, Chee Siean *, et al.
Published: (2018)

Inhibitory activity of cyclohexenyl chalcone derivatives and flavonoids of fingerroot, Boesenbergia rotunda (L.), towards dengue-2 virus NS3 protease.
by: Tan, Siew Kiat *, et al.
Published: (2006)

Quantitative forecasting trend of dengue fever and dengue haemorrhagic fever cases in Peninsular Malaysia
by: Cheng, Kueh Yee
Published: (2006)

Construction and expression of synthetic gene encoding MPT64 as extracellular protein in Escherichia coli BL21 (DE3) expression system
by: Kusuma, Sri Agung Fitri, et al.
Published: (2018)

Controlling dengue: an uphill task?
by: Amjad, Nasser Muhammad
Published: (2015)

Dengue in Malaysia - a commentary
by: Abd. Kadir, Abu Hassan Shaari, et al.
Published: (2015)

Development of peptide vaccines in dengue
by: Reginald, Kavita*, et al.
Published: (2017)

Phylodynamics of dengue virus in Malaysia
by: Sazaly, AB
Published: (2011)

Birdcages source of dengue virus
by: Medical, Xpress
Published: (2016)

Dengue vaccine price soars
by: Vethasalam, Ragananthini
Published: (2024)