Isolation and screening of an extracellular organic solvent-tolerant protease producer
An extracellular organic solvent-tolerant protease producer has been successfully isolated out of 11 isolates of benzene-toluene-xylene-ethylbenzene (BTEX) tolerant bacteria. This organic solvent-tolerant microorganism was found to be a polycyclic-aromatic-hydrocarbons (PAHs) degrader and identified...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier Science B.V.
2003
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/114458/ http://psasir.upm.edu.my/id/eprint/114458/1/114458.pdf |
| _version_ | 1848866500679565312 |
|---|---|
| author | Geok, Lee Poh Nyonya, Che Razak, Abdul Abd Rahman, Raja Noor Zaliha |
| author_facet | Geok, Lee Poh Nyonya, Che Razak, Abdul Abd Rahman, Raja Noor Zaliha |
| author_sort | Geok, Lee Poh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An extracellular organic solvent-tolerant protease producer has been successfully isolated out of 11 isolates of benzene-toluene-xylene-ethylbenzene (BTEX) tolerant bacteria. This organic solvent-tolerant microorganism was found to be a polycyclic-aromatic-hydrocarbons (PAHs) degrader and identified as Pseudomonas aeruginosa strain K. It was selected based on the stability of its proteolytic enzyme in the presence of various organic solvents. P. aeruginosa strain K protease tolerated up to at least 50% (v/v) of benzene, n-hexane, 1-decanol, isooctane and n-hexadecane and was also stable in the presence of 25% (v/v) n-decane and n-dodecane. This enzyme strain K was activated 2.5, 1.5 and 1.2 times by 75% (v/v) of 1-decanol, isooctane and n-dodecane, respectively. This organic solvent stable protease could be used as a biocatalyst for enzymatic synthesis in the presence of organic solvents. |
| first_indexed | 2025-11-15T14:21:36Z |
| format | Article |
| id | upm-114458 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:21:36Z |
| publishDate | 2003 |
| publisher | Elsevier Science B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1144582025-02-05T03:55:37Z http://psasir.upm.edu.my/id/eprint/114458/ Isolation and screening of an extracellular organic solvent-tolerant protease producer Geok, Lee Poh Nyonya, Che Razak, Abdul Abd Rahman, Raja Noor Zaliha An extracellular organic solvent-tolerant protease producer has been successfully isolated out of 11 isolates of benzene-toluene-xylene-ethylbenzene (BTEX) tolerant bacteria. This organic solvent-tolerant microorganism was found to be a polycyclic-aromatic-hydrocarbons (PAHs) degrader and identified as Pseudomonas aeruginosa strain K. It was selected based on the stability of its proteolytic enzyme in the presence of various organic solvents. P. aeruginosa strain K protease tolerated up to at least 50% (v/v) of benzene, n-hexane, 1-decanol, isooctane and n-hexadecane and was also stable in the presence of 25% (v/v) n-decane and n-dodecane. This enzyme strain K was activated 2.5, 1.5 and 1.2 times by 75% (v/v) of 1-decanol, isooctane and n-dodecane, respectively. This organic solvent stable protease could be used as a biocatalyst for enzymatic synthesis in the presence of organic solvents. Elsevier Science B.V. 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114458/1/114458.pdf Geok, Lee Poh and Nyonya, Che and Razak, Abdul and Abd Rahman, Raja Noor Zaliha (2003) Isolation and screening of an extracellular organic solvent-tolerant protease producer. Biochemical Engineering Journal, 13 (1). pp. 73-77. ISSN 1369-703X; eISSN: 1369-703X https://linkinghub.elsevier.com/retrieve/pii/S1369703X02001377 10.1016/S1369-703X(02)00137-7 |
| spellingShingle | Geok, Lee Poh Nyonya, Che Razak, Abdul Abd Rahman, Raja Noor Zaliha Isolation and screening of an extracellular organic solvent-tolerant protease producer |
| title | Isolation and screening of an extracellular organic solvent-tolerant protease producer |
| title_full | Isolation and screening of an extracellular organic solvent-tolerant protease producer |
| title_fullStr | Isolation and screening of an extracellular organic solvent-tolerant protease producer |
| title_full_unstemmed | Isolation and screening of an extracellular organic solvent-tolerant protease producer |
| title_short | Isolation and screening of an extracellular organic solvent-tolerant protease producer |
| title_sort | isolation and screening of an extracellular organic solvent-tolerant protease producer |
| url | http://psasir.upm.edu.my/id/eprint/114458/ http://psasir.upm.edu.my/id/eprint/114458/ http://psasir.upm.edu.my/id/eprint/114458/ http://psasir.upm.edu.my/id/eprint/114458/1/114458.pdf |