Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells
Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii, induced apoptosis in Jurkat T-cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases-3 and -7 with the appearance of their catalytically active large subunits...
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| Format: | Article |
| Language: | English |
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Federation of European Biochemical Societies
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114448/ http://psasir.upm.edu.my/id/eprint/114448/1/114448.pdf |
| _version_ | 1848866497884061696 |
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| author | Inayat-Hussain, Salmaan H. Osman, Annuar B. Din, Laily B. Ali, Abdul M. |
| author_facet | Inayat-Hussain, Salmaan H. Osman, Annuar B. Din, Laily B. Ali, Abdul M. |
| author_sort | Inayat-Hussain, Salmaan H. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii, induced apoptosis in Jurkat T-cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases-3 and -7 with the appearance of their catalytically active large subunits of 17 and 19 kDa, respectively. Activation of these caspases was further evidenced by detection of poly(ADP-ribose) polymerase cleavage (PARP). Pre-treatment with the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (Z-VAD.FMK) blocked apoptosis and the resultant cleavage of these caspases and PARP. Our results demonstrate that activation of at least two effector caspases is a key feature of goniothalamin-induced apoptosis in Jurkat T-cells. |
| first_indexed | 2025-11-15T14:21:33Z |
| format | Article |
| id | upm-114448 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:21:33Z |
| publishDate | 1999 |
| publisher | Federation of European Biochemical Societies |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1144482025-01-15T07:40:07Z http://psasir.upm.edu.my/id/eprint/114448/ Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells Inayat-Hussain, Salmaan H. Osman, Annuar B. Din, Laily B. Ali, Abdul M. Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii, induced apoptosis in Jurkat T-cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases-3 and -7 with the appearance of their catalytically active large subunits of 17 and 19 kDa, respectively. Activation of these caspases was further evidenced by detection of poly(ADP-ribose) polymerase cleavage (PARP). Pre-treatment with the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (Z-VAD.FMK) blocked apoptosis and the resultant cleavage of these caspases and PARP. Our results demonstrate that activation of at least two effector caspases is a key feature of goniothalamin-induced apoptosis in Jurkat T-cells. Federation of European Biochemical Societies 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114448/1/114448.pdf Inayat-Hussain, Salmaan H. and Osman, Annuar B. and Din, Laily B. and Ali, Abdul M. (1999) Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells. FEBS Letters, 456 (3). pp. 379-383. ISSN 0014-5793; eISSN: 0014-5793 https://febs.onlinelibrary.wiley.com/doi/10.1016/S0014-5793%2899%2900984-9 10.1016/S0014-5793(99)00984-9 |
| spellingShingle | Inayat-Hussain, Salmaan H. Osman, Annuar B. Din, Laily B. Ali, Abdul M. Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells |
| title | Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells |
| title_full | Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells |
| title_fullStr | Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells |
| title_full_unstemmed | Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells |
| title_short | Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells |
| title_sort | caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human jurkat t-cells |
| url | http://psasir.upm.edu.my/id/eprint/114448/ http://psasir.upm.edu.my/id/eprint/114448/ http://psasir.upm.edu.my/id/eprint/114448/ http://psasir.upm.edu.my/id/eprint/114448/1/114448.pdf |