Microbiological And Physico-Chemical Changes During Fermentation Of Theobroma Cacao, L.: Isolation And Characterization Of Cocoa Lipase.

A study was carried out to examine microbiological and physicochemical changes during the fermentation of Theobroma cacao. Isolation, partial purification, characterization of cocoa lipase were also carried out. Results showed that several microbes were successfully detected during fermentation...

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Main Author: Samsumaharto, Ratna Agung
Format: Thesis
Language:English
English
Published: 2000
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/11440/
http://psasir.upm.edu.my/id/eprint/11440/1/FSAS_2000_27.pdf
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author Samsumaharto, Ratna Agung
author_facet Samsumaharto, Ratna Agung
author_sort Samsumaharto, Ratna Agung
building UPM Institutional Repository
collection Online Access
description A study was carried out to examine microbiological and physicochemical changes during the fermentation of Theobroma cacao. Isolation, partial purification, characterization of cocoa lipase were also carried out. Results showed that several microbes were successfully detected during fermentation of cocoa beans. The maximum number of yeast colonies observed in fermented cocoa beans PBC 123 and 159 clones was 4.7 x 108 and 1.5 x 109 CFU/g. fro wt., respectively. Acetic acid bacteria we·re found to be dominant, 9.6 x 107 CFU/g. fro wt. for PBC 123 clone and 1.4 x 108 CFU/g. fro wt. for PBC 159 clone during the third day of fermentation whilst moulds were present throughout the fermentation period of six days. Spore forming bacteria appeared to be dominant on the fifth day of fermentation period. The maximum number of colonies observed for lactic acid bacteria was 3.5 x 106
first_indexed 2025-11-15T07:46:19Z
format Thesis
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institution Universiti Putra Malaysia
institution_category Local University
language English
English
last_indexed 2025-11-15T07:46:19Z
publishDate 2000
recordtype eprints
repository_type Digital Repository
spelling upm-114402024-06-19T07:29:34Z http://psasir.upm.edu.my/id/eprint/11440/ Microbiological And Physico-Chemical Changes During Fermentation Of Theobroma Cacao, L.: Isolation And Characterization Of Cocoa Lipase. Samsumaharto, Ratna Agung A study was carried out to examine microbiological and physicochemical changes during the fermentation of Theobroma cacao. Isolation, partial purification, characterization of cocoa lipase were also carried out. Results showed that several microbes were successfully detected during fermentation of cocoa beans. The maximum number of yeast colonies observed in fermented cocoa beans PBC 123 and 159 clones was 4.7 x 108 and 1.5 x 109 CFU/g. fro wt., respectively. Acetic acid bacteria we·re found to be dominant, 9.6 x 107 CFU/g. fro wt. for PBC 123 clone and 1.4 x 108 CFU/g. fro wt. for PBC 159 clone during the third day of fermentation whilst moulds were present throughout the fermentation period of six days. Spore forming bacteria appeared to be dominant on the fifth day of fermentation period. The maximum number of colonies observed for lactic acid bacteria was 3.5 x 106 2000-02 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/11440/1/FSAS_2000_27.pdf Samsumaharto, Ratna Agung (2000) Microbiological And Physico-Chemical Changes During Fermentation Of Theobroma Cacao, L.: Isolation And Characterization Of Cocoa Lipase. Masters thesis, Universiti Putra Malaysia. Fermentation Cacao Microbiology - Research English
spellingShingle Fermentation
Cacao
Microbiology - Research
Samsumaharto, Ratna Agung
Microbiological And Physico-Chemical Changes During Fermentation Of Theobroma Cacao, L.: Isolation And Characterization Of Cocoa Lipase.
title Microbiological And Physico-Chemical Changes During Fermentation Of Theobroma Cacao, L.: Isolation And Characterization Of Cocoa Lipase.
title_full Microbiological And Physico-Chemical Changes During Fermentation Of Theobroma Cacao, L.: Isolation And Characterization Of Cocoa Lipase.
title_fullStr Microbiological And Physico-Chemical Changes During Fermentation Of Theobroma Cacao, L.: Isolation And Characterization Of Cocoa Lipase.
title_full_unstemmed Microbiological And Physico-Chemical Changes During Fermentation Of Theobroma Cacao, L.: Isolation And Characterization Of Cocoa Lipase.
title_short Microbiological And Physico-Chemical Changes During Fermentation Of Theobroma Cacao, L.: Isolation And Characterization Of Cocoa Lipase.
title_sort microbiological and physico-chemical changes during fermentation of theobroma cacao, l.: isolation and characterization of cocoa lipase.
topic Fermentation
Cacao
Microbiology - Research
url http://psasir.upm.edu.my/id/eprint/11440/
http://psasir.upm.edu.my/id/eprint/11440/1/FSAS_2000_27.pdf