Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl
Cloning of alkaline protease gene from a thermophilic bacteria, Bacillus stearothermophilus Fl (BSF1) was done by PCR cloning method. Genomic DNA of BSFI was extracted and the highest concentration obtained was 0.46 ug/uL and with a purity of 2.0. Three sets of primers were used including RM5 (5&...
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| Format: | Thesis |
| Language: | English English |
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2000
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11436/ http://psasir.upm.edu.my/id/eprint/11436/1/FSAS_2000_25.pdf |
| _version_ | 1848841631149588480 |
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| author | Ab.Hamid, Suhaili |
| author_facet | Ab.Hamid, Suhaili |
| author_sort | Ab.Hamid, Suhaili |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cloning of alkaline protease gene from a thermophilic bacteria, Bacillus
stearothermophilus Fl (BSF1) was done by PCR cloning method. Genomic DNA
of BSFI was extracted and the highest concentration obtained was 0.46 ug/uL and
with a purity of 2.0. Three sets of primers were used including RM5 (5'-CA(CT)
GG(ACGT) ACC AA(CT) GTG GC(CGT) GG-3) and RM6 (5'-(ACG)GG GGT
(ACG)GC CAT GGA (CGT)CC-3', F0R900 (5-GCA TGC TAC GAT TAA ATA
TC-3) and REV900 (5'-CGG CAA TAT CAC ITA GAG TAC C-3') and FOR900
(5'-GCA TGC TAC GAT TAA ATA TC-3') and REV1 591 (5-TGC AGC AGA
AAG AAG GAA-3') which resuhed in amplification of 500-bp, 900-bp and 1 500-
bp products, respectively. Southern blotting analysis suggested that both 500-bp
and 900-bp fragment were present within the 1 500-bp fragment. E. coli TOPIOF |
| first_indexed | 2025-11-15T07:46:18Z |
| format | Thesis |
| id | upm-11436 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:46:18Z |
| publishDate | 2000 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-114362024-06-19T07:26:35Z http://psasir.upm.edu.my/id/eprint/11436/ Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl Ab.Hamid, Suhaili Cloning of alkaline protease gene from a thermophilic bacteria, Bacillus stearothermophilus Fl (BSF1) was done by PCR cloning method. Genomic DNA of BSFI was extracted and the highest concentration obtained was 0.46 ug/uL and with a purity of 2.0. Three sets of primers were used including RM5 (5'-CA(CT) GG(ACGT) ACC AA(CT) GTG GC(CGT) GG-3) and RM6 (5'-(ACG)GG GGT (ACG)GC CAT GGA (CGT)CC-3', F0R900 (5-GCA TGC TAC GAT TAA ATA TC-3) and REV900 (5'-CGG CAA TAT CAC ITA GAG TAC C-3') and FOR900 (5'-GCA TGC TAC GAT TAA ATA TC-3') and REV1 591 (5-TGC AGC AGA AAG AAG GAA-3') which resuhed in amplification of 500-bp, 900-bp and 1 500- bp products, respectively. Southern blotting analysis suggested that both 500-bp and 900-bp fragment were present within the 1 500-bp fragment. E. coli TOPIOF 2000-06 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/11436/1/FSAS_2000_25.pdf Ab.Hamid, Suhaili (2000) Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl. Masters thesis, Universiti Putra Malaysia. Molecular cloning English |
| spellingShingle | Molecular cloning Ab.Hamid, Suhaili Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl |
| title | Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl |
| title_full | Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl |
| title_fullStr | Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl |
| title_full_unstemmed | Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl |
| title_short | Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl |
| title_sort | cloning and expression of alkaline protease gene from bacillus stearothermophilus strain fl |
| topic | Molecular cloning |
| url | http://psasir.upm.edu.my/id/eprint/11436/ http://psasir.upm.edu.my/id/eprint/11436/1/FSAS_2000_25.pdf |