A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase
A total of 131 organic solvent tolerant isolates originating from soil samples were successfully isolated via direct plating method using 1% (v/v) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source. Six isolates demonstrated high tolerance of up to 75% (v/v) co...
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| Format: | Article |
| Language: | English |
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Elsevier Science B.V.
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114350/ http://psasir.upm.edu.my/id/eprint/114350/1/114350.pdf |
| _version_ | 1848866467181756416 |
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| author | Hun, Chin John Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_facet | Hun, Chin John Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_sort | Hun, Chin John |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A total of 131 organic solvent tolerant isolates originating from soil samples were successfully isolated via direct plating method using 1% (v/v) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source. Six isolates demonstrated high tolerance of up to 75% (v/v) concentration of BTEX (n-benzene, toluene, ethyl benzene and p-xylene). These isolates were screened for lipolytic activity using triolein plates. Isolate 205y was found to produce the highest lipase yield in liquid medium and later identified as Bacillus sphaericus by biochemical test and 16S ribosomal DNA sequence. No work on B. sphaericus producing lipase has been reported so far. Four production media were tested and medium M3 yielded the highest lipase activity of 0.42 U ml-1 min-1 at 36 h incubation time. Stability studies in various organic solvents at 25% (v/v) showed that lipase from B. sphaericus 205y were not only stable but also activated by n-hexane and p-xylene by 3.5- and 2.9-folds, respectively. The activity, however, was greatly reduced in dimethyl sulfoxide and was completely inactivated by hexadecane and acetonitrile. |
| first_indexed | 2025-11-15T14:21:04Z |
| format | Article |
| id | upm-114350 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:21:04Z |
| publishDate | 2003 |
| publisher | Elsevier Science B.V. |
| recordtype | eprints |
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| spelling | upm-1143502025-02-05T03:34:17Z http://psasir.upm.edu.my/id/eprint/114350/ A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase Hun, Chin John Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran A total of 131 organic solvent tolerant isolates originating from soil samples were successfully isolated via direct plating method using 1% (v/v) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source. Six isolates demonstrated high tolerance of up to 75% (v/v) concentration of BTEX (n-benzene, toluene, ethyl benzene and p-xylene). These isolates were screened for lipolytic activity using triolein plates. Isolate 205y was found to produce the highest lipase yield in liquid medium and later identified as Bacillus sphaericus by biochemical test and 16S ribosomal DNA sequence. No work on B. sphaericus producing lipase has been reported so far. Four production media were tested and medium M3 yielded the highest lipase activity of 0.42 U ml-1 min-1 at 36 h incubation time. Stability studies in various organic solvents at 25% (v/v) showed that lipase from B. sphaericus 205y were not only stable but also activated by n-hexane and p-xylene by 3.5- and 2.9-folds, respectively. The activity, however, was greatly reduced in dimethyl sulfoxide and was completely inactivated by hexadecane and acetonitrile. Elsevier Science B.V. 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114350/1/114350.pdf Hun, Chin John and Raja Abd Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2003) A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase. Biochemical Engineering Journal, 15 (2). pp. 147-151. ISSN 1369-703X; eISSN: 1369-703X https://linkinghub.elsevier.com/retrieve/pii/S1369703X02001857 10.1016/S1369-703X(02)00185-7 |
| spellingShingle | Hun, Chin John Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase |
| title | A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase |
| title_full | A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase |
| title_fullStr | A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase |
| title_full_unstemmed | A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase |
| title_short | A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase |
| title_sort | newly isolated organic solvent tolerant bacillus sphaericus 205y producing organic solvent-stable lipase |
| url | http://psasir.upm.edu.my/id/eprint/114350/ http://psasir.upm.edu.my/id/eprint/114350/ http://psasir.upm.edu.my/id/eprint/114350/ http://psasir.upm.edu.my/id/eprint/114350/1/114350.pdf |