Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens
1. 1. The effects of α-tocopherol and γ-tocotrienol on glutathione S-transferase (GST) and γ-glutamyl transpeptidase (γ-GT) activities in cultured hepatocytes prepared from rats treated with diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) were investigated. 2. 2. Both the α-tocopherol and γ...
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| Format: | Article |
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Elsevier
1994
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114063/ http://psasir.upm.edu.my/id/eprint/114063/1/114063.pdf |
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| author | Ong, F.B. Wan Ngah, W.Z. Top, A.G.M. Khaud, B.A.K. Shamaan, N.A. |
| author_facet | Ong, F.B. Wan Ngah, W.Z. Top, A.G.M. Khaud, B.A.K. Shamaan, N.A. |
| author_sort | Ong, F.B. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | 1. 1. The effects of α-tocopherol and γ-tocotrienol on glutathione S-transferase (GST) and γ-glutamyl transpeptidase (γ-GT) activities in cultured hepatocytes prepared from rats treated with diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) were investigated. 2. 2. Both the α-tocopherol and γ-tocotrienol treated hepatocytes showed significantly higher (P < 0.05) GST activities than untreated hepatocytes prepared from the carcinogen treated rats in the first 3 days of culture. Treatment with α-tocopherol and γ-tocotrienol generally resulted in a tendency to increase the GST activities above that in the untreated hepatocytes. 3. 3. Treatment with high doses (125-250 μM) of α-tocopherol and low doses (12.5-25 μM) of γ-tocotrienol generally resulted in a significant reduction in γ-GT activities at 1-3 days. γ-GT activities are reduced as the dose of α-tocopherol and γ-tocotrienol are increased. |
| first_indexed | 2025-11-15T14:19:54Z |
| format | Article |
| id | upm-114063 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:19:54Z |
| publishDate | 1994 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1140632024-12-02T07:14:45Z http://psasir.upm.edu.my/id/eprint/114063/ Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens Ong, F.B. Wan Ngah, W.Z. Top, A.G.M. Khaud, B.A.K. Shamaan, N.A. 1. 1. The effects of α-tocopherol and γ-tocotrienol on glutathione S-transferase (GST) and γ-glutamyl transpeptidase (γ-GT) activities in cultured hepatocytes prepared from rats treated with diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) were investigated. 2. 2. Both the α-tocopherol and γ-tocotrienol treated hepatocytes showed significantly higher (P < 0.05) GST activities than untreated hepatocytes prepared from the carcinogen treated rats in the first 3 days of culture. Treatment with α-tocopherol and γ-tocotrienol generally resulted in a tendency to increase the GST activities above that in the untreated hepatocytes. 3. 3. Treatment with high doses (125-250 μM) of α-tocopherol and low doses (12.5-25 μM) of γ-tocotrienol generally resulted in a significant reduction in γ-GT activities at 1-3 days. γ-GT activities are reduced as the dose of α-tocopherol and γ-tocotrienol are increased. Elsevier 1994 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114063/1/114063.pdf Ong, F.B. and Wan Ngah, W.Z. and Top, A.G.M. and Khaud, B.A.K. and Shamaan, N.A. (1994) Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens. International Journal of Biochemistry, 26 (3). pp. 397-402. ISSN 0020-711X; eISSN: 0020-711X https://linkinghub.elsevier.com/retrieve/pii/0020711X94900604 10.1016/0020-711x(94)90060-4 |
| spellingShingle | Ong, F.B. Wan Ngah, W.Z. Top, A.G.M. Khaud, B.A.K. Shamaan, N.A. Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens |
| title | Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens |
| title_full | Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens |
| title_fullStr | Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens |
| title_full_unstemmed | Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens |
| title_short | Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens |
| title_sort | vitamin e, glutathione s-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens |
| url | http://psasir.upm.edu.my/id/eprint/114063/ http://psasir.upm.edu.my/id/eprint/114063/ http://psasir.upm.edu.my/id/eprint/114063/ http://psasir.upm.edu.my/id/eprint/114063/1/114063.pdf |