Antiallergic effect of the alpha‐cyclodextrin Moringin complex in rat basophilic leukaemia (RBL‐2H3) cell line
Allergic diseases (ADs) are a major concern when it comes to public well-being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(α-L-rhamnopyranosyloxy)-benzyl isothio...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/113904/ http://psasir.upm.edu.my/id/eprint/113904/1/113904.pdf |
| _version_ | 1848866353481515008 |
|---|---|
| author | Yousef A. Alnakeeb, Ebtisam Abdull Razis, Ahmad Faizal Wei Chan, Kim Ling Tham, Chau Han Chan, Yee Kalifa Kafo, Anwar Salm Jambari, Nuzul Noorahya Rollin, Patrick Djedaini-Pilard, Florence Mutalik, Srinivas |
| author_facet | Yousef A. Alnakeeb, Ebtisam Abdull Razis, Ahmad Faizal Wei Chan, Kim Ling Tham, Chau Han Chan, Yee Kalifa Kafo, Anwar Salm Jambari, Nuzul Noorahya Rollin, Patrick Djedaini-Pilard, Florence Mutalik, Srinivas |
| author_sort | Yousef A. Alnakeeb, Ebtisam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Allergic diseases (ADs) are a major concern when it comes to public well-being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(α-L-rhamnopyranosyloxy)-benzyl isothiocyanate or moringin isolated from Moringa oleifera seeds in the form of alpha-cyclodextrin-moringin (α-CD/MG) complex on rat basophilic leukaemia (RBL-2H3) cell line at both the early and late stages of an allergic reaction. The α-CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and α-CD/MG. The release of beta-hexosaminidase (β-hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor-alpha (TNF-α), and interleukin (IL-4) was considered in the late stage. Higher concentrations of α-CD/MG (5 μM, p<0.001) in mast cell degranulation significantly inhibited the expression of β-hexosaminidase, histamine, TNF-α, PGD2, and IL-4 in both the early and late stages. Thus, α-CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages. |
| first_indexed | 2025-11-15T14:19:15Z |
| format | Article |
| id | upm-113904 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:19:15Z |
| publishDate | 2024 |
| publisher | Hindawi Limited |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1139042024-11-25T02:28:48Z http://psasir.upm.edu.my/id/eprint/113904/ Antiallergic effect of the alpha‐cyclodextrin Moringin complex in rat basophilic leukaemia (RBL‐2H3) cell line Yousef A. Alnakeeb, Ebtisam Abdull Razis, Ahmad Faizal Wei Chan, Kim Ling Tham, Chau Han Chan, Yee Kalifa Kafo, Anwar Salm Jambari, Nuzul Noorahya Rollin, Patrick Djedaini-Pilard, Florence Mutalik, Srinivas Allergic diseases (ADs) are a major concern when it comes to public well-being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4-(α-L-rhamnopyranosyloxy)-benzyl isothiocyanate or moringin isolated from Moringa oleifera seeds in the form of alpha-cyclodextrin-moringin (α-CD/MG) complex on rat basophilic leukaemia (RBL-2H3) cell line at both the early and late stages of an allergic reaction. The α-CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and α-CD/MG. The release of beta-hexosaminidase (β-hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor-alpha (TNF-α), and interleukin (IL-4) was considered in the late stage. Higher concentrations of α-CD/MG (5 μM, p<0.001) in mast cell degranulation significantly inhibited the expression of β-hexosaminidase, histamine, TNF-α, PGD2, and IL-4 in both the early and late stages. Thus, α-CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages. Hindawi Limited 2024 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/113904/1/113904.pdf Yousef A. Alnakeeb, Ebtisam and Abdull Razis, Ahmad Faizal and Wei Chan, Kim and Ling Tham, Chau and Han Chan, Yee and Kalifa Kafo, Anwar Salm and Jambari, Nuzul Noorahya and Rollin, Patrick and Djedaini-Pilard, Florence and Mutalik, Srinivas (2024) Antiallergic effect of the alpha‐cyclodextrin Moringin complex in rat basophilic leukaemia (RBL‐2H3) cell line. Advances in Pharmacological and Pharmaceutical Sciences, 2024. art. no. 8885068. ISSN 2633-4682; eISSN: 2633-4690 https://onlinelibrary.wiley.com/doi/10.1155/2024/8885068 10.1155/2024/8885068 |
| spellingShingle | Yousef A. Alnakeeb, Ebtisam Abdull Razis, Ahmad Faizal Wei Chan, Kim Ling Tham, Chau Han Chan, Yee Kalifa Kafo, Anwar Salm Jambari, Nuzul Noorahya Rollin, Patrick Djedaini-Pilard, Florence Mutalik, Srinivas Antiallergic effect of the alpha‐cyclodextrin Moringin complex in rat basophilic leukaemia (RBL‐2H3) cell line |
| title | Antiallergic effect of the alpha‐cyclodextrin Moringin complex in rat basophilic leukaemia (RBL‐2H3) cell line |
| title_full | Antiallergic effect of the alpha‐cyclodextrin Moringin complex in rat basophilic leukaemia (RBL‐2H3) cell line |
| title_fullStr | Antiallergic effect of the alpha‐cyclodextrin Moringin complex in rat basophilic leukaemia (RBL‐2H3) cell line |
| title_full_unstemmed | Antiallergic effect of the alpha‐cyclodextrin Moringin complex in rat basophilic leukaemia (RBL‐2H3) cell line |
| title_short | Antiallergic effect of the alpha‐cyclodextrin Moringin complex in rat basophilic leukaemia (RBL‐2H3) cell line |
| title_sort | antiallergic effect of the alpha‐cyclodextrin moringin complex in rat basophilic leukaemia (rbl‐2h3) cell line |
| url | http://psasir.upm.edu.my/id/eprint/113904/ http://psasir.upm.edu.my/id/eprint/113904/ http://psasir.upm.edu.my/id/eprint/113904/ http://psasir.upm.edu.my/id/eprint/113904/1/113904.pdf |