Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique
Genomic DNA of sergestid shrimp, Acetes japonicus was successfully extracted by using the Promega Wizard Genomic DNA Purification Kit. Population genetic characterization of A. japonicus along the western coast of Peninsular Malaysia (state of Perak, Malacca and Kedah) was examined using the Random...
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| Format: | Article |
| Language: | English English |
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2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11388/ http://psasir.upm.edu.my/id/eprint/11388/1/Population_Characterization_of_Planktonic_Shrimp.pdf |
| _version_ | 1848841625167462400 |
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| author | Deraman, Aziz Siraj, Siti Shapor Arshad, Aziz Nurul Amin, Sanker Mohammad Harmin, Sharr Azni |
| author_facet | Deraman, Aziz Siraj, Siti Shapor Arshad, Aziz Nurul Amin, Sanker Mohammad Harmin, Sharr Azni |
| author_sort | Deraman, Aziz |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Genomic DNA of sergestid shrimp, Acetes japonicus was successfully extracted by using the Promega Wizard Genomic DNA Purification Kit. Population genetic characterization of A. japonicus along the western coast of Peninsular Malaysia (state of Perak, Malacca and Kedah) was examined using the Random Amplified Polymorphic DNA (RAPD) marker. Twenty oligonucleotides from operon A kit were used to screen the populations, of which 6 were able to be amplified (OPA03, OPA04, OPA07, OPA09, OPA10, OPA16). The percentages of polymorphic bands of the three populations investigated varied from 57.77 to 87.77%. Genetic distances between populations and cluster analysis from UPGMA grouped the populations into two major clusters. The Perak and Malacca populations were in one cluster, while the Kedah population was clustered by itself, indicating a different population. The genetic distance was highest as 0.0999 for the Kedah and the Malacca populations while lowest value was 0.0413 for the Perak and the Malacca populations, which probably have a closed ancestral relationship. The results of this study suggested that RAPD analysis, if carried out carefully and accurately would give a good indication of the separation between individuals of different populations and is suitable for identification of closely related genotypes. |
| first_indexed | 2025-11-15T07:46:12Z |
| format | Article |
| id | upm-11388 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:46:12Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-113882013-05-27T07:48:49Z http://psasir.upm.edu.my/id/eprint/11388/ Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique Deraman, Aziz Siraj, Siti Shapor Arshad, Aziz Nurul Amin, Sanker Mohammad Harmin, Sharr Azni Genomic DNA of sergestid shrimp, Acetes japonicus was successfully extracted by using the Promega Wizard Genomic DNA Purification Kit. Population genetic characterization of A. japonicus along the western coast of Peninsular Malaysia (state of Perak, Malacca and Kedah) was examined using the Random Amplified Polymorphic DNA (RAPD) marker. Twenty oligonucleotides from operon A kit were used to screen the populations, of which 6 were able to be amplified (OPA03, OPA04, OPA07, OPA09, OPA10, OPA16). The percentages of polymorphic bands of the three populations investigated varied from 57.77 to 87.77%. Genetic distances between populations and cluster analysis from UPGMA grouped the populations into two major clusters. The Perak and Malacca populations were in one cluster, while the Kedah population was clustered by itself, indicating a different population. The genetic distance was highest as 0.0999 for the Kedah and the Malacca populations while lowest value was 0.0413 for the Perak and the Malacca populations, which probably have a closed ancestral relationship. The results of this study suggested that RAPD analysis, if carried out carefully and accurately would give a good indication of the separation between individuals of different populations and is suitable for identification of closely related genotypes. 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11388/1/Population_Characterization_of_Planktonic_Shrimp.pdf Deraman, Aziz and Siraj, Siti Shapor and Arshad, Aziz and Nurul Amin, Sanker Mohammad and Harmin, Sharr Azni (2010) Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique. Journal of Biological Sciences, 10 (4). pp. 355-361. ISSN 1727-3048 10.3923/jbs.2010.355.361 English |
| spellingShingle | Deraman, Aziz Siraj, Siti Shapor Arshad, Aziz Nurul Amin, Sanker Mohammad Harmin, Sharr Azni Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique |
| title | Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique |
| title_full | Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique |
| title_fullStr | Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique |
| title_full_unstemmed | Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique |
| title_short | Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique |
| title_sort | population characterization of planktonic shrimp, acetes japonicus (decapoda: sergestidae) using rapd technique |
| url | http://psasir.upm.edu.my/id/eprint/11388/ http://psasir.upm.edu.my/id/eprint/11388/ http://psasir.upm.edu.my/id/eprint/11388/1/Population_Characterization_of_Planktonic_Shrimp.pdf |