The potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis
Leptospirosis, a notifiable endemic disease in Malaysia, has higher mortality rates than regional dengue fever. Diverse clinical symptoms and limited diagnostic methods complicate leptospirosis diagnosis. The demand for accurate biomarker-based diagnostics is increasing. This study investigated the...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
American Chemical Society
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/113864/ |
| _version_ | 1848866343868170240 |
|---|---|
| author | Fish-Low, Cheng-Yee Than, Leslie Thian Lung Ling, King-Hwa Sekawi, Zamberi |
| author_facet | Fish-Low, Cheng-Yee Than, Leslie Thian Lung Ling, King-Hwa Sekawi, Zamberi |
| author_sort | Fish-Low, Cheng-Yee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Leptospirosis, a notifiable endemic disease in Malaysia, has higher mortality rates than regional dengue fever. Diverse clinical symptoms and limited diagnostic methods complicate leptospirosis diagnosis. The demand for accurate biomarker-based diagnostics is increasing. This study investigated the plasma proteome of leptospirosis patients with leptospiraemia and seroconversion compared with dengue patients and healthy subjects using isobaric tags for relative and absolute quantitation (iTRAQ)-mass spectrometry (MS). The iTRAQ analysis identified a total of 450 proteins, which were refined to a list of 290 proteins through a series of exclusion criteria. Differential expression in the plasma proteome of leptospirosis patients compared to the control groups identified 11 proteins, which are apolipoprotein A-II (APOA2), C-reactive protein (CRP), fermitin family homolog 3 (FERMT3), leucine-rich alpha-2-glycoprotein 1 (LRG1), lipopolysaccharide-binding protein (LBP), myosin-9 (MYH9), platelet basic protein (PPBP), platelet factor 4 (PF4), profilin-1 (PFN1), serum amyloid A-1 protein (SAA1), and thrombospondin-1 (THBS1). Following a study on a verification cohort, a panel of eight plasma protein biomarkers was identified for potential leptospirosis diagnosis: CRP, LRG1, LBP, MYH9, PPBP, PF4, SAA1, and THBS1. In conclusion, a panel of eight protein biomarkers offers a promising approach for leptospirosis diagnosis, addressing the limitations of the “one disease, one biomarker” concept. |
| first_indexed | 2025-11-15T14:19:06Z |
| format | Article |
| id | upm-113864 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:19:06Z |
| publishDate | 2024 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1138642025-02-07T07:16:57Z http://psasir.upm.edu.my/id/eprint/113864/ The potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis Fish-Low, Cheng-Yee Than, Leslie Thian Lung Ling, King-Hwa Sekawi, Zamberi Leptospirosis, a notifiable endemic disease in Malaysia, has higher mortality rates than regional dengue fever. Diverse clinical symptoms and limited diagnostic methods complicate leptospirosis diagnosis. The demand for accurate biomarker-based diagnostics is increasing. This study investigated the plasma proteome of leptospirosis patients with leptospiraemia and seroconversion compared with dengue patients and healthy subjects using isobaric tags for relative and absolute quantitation (iTRAQ)-mass spectrometry (MS). The iTRAQ analysis identified a total of 450 proteins, which were refined to a list of 290 proteins through a series of exclusion criteria. Differential expression in the plasma proteome of leptospirosis patients compared to the control groups identified 11 proteins, which are apolipoprotein A-II (APOA2), C-reactive protein (CRP), fermitin family homolog 3 (FERMT3), leucine-rich alpha-2-glycoprotein 1 (LRG1), lipopolysaccharide-binding protein (LBP), myosin-9 (MYH9), platelet basic protein (PPBP), platelet factor 4 (PF4), profilin-1 (PFN1), serum amyloid A-1 protein (SAA1), and thrombospondin-1 (THBS1). Following a study on a verification cohort, a panel of eight plasma protein biomarkers was identified for potential leptospirosis diagnosis: CRP, LRG1, LBP, MYH9, PPBP, PF4, SAA1, and THBS1. In conclusion, a panel of eight protein biomarkers offers a promising approach for leptospirosis diagnosis, addressing the limitations of the “one disease, one biomarker” concept. American Chemical Society 2024-08-16 Article PeerReviewed Fish-Low, Cheng-Yee and Than, Leslie Thian Lung and Ling, King-Hwa and Sekawi, Zamberi (2024) The potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis. Journal of Proteome Research, 23 (9). pp. 4027-4042. ISSN 1535-3893; eISSN: 1535-3907 https://pubs.acs.org/doi/10.1021/acs.jproteome.4c00376 10.1021/acs.jproteome.4c00376 |
| spellingShingle | Fish-Low, Cheng-Yee Than, Leslie Thian Lung Ling, King-Hwa Sekawi, Zamberi The potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis |
| title | The potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis |
| title_full | The potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis |
| title_fullStr | The potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis |
| title_full_unstemmed | The potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis |
| title_short | The potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis |
| title_sort | potential of eight plasma proteins as biomarkers in redefining leptospirosis diagnosis |
| url | http://psasir.upm.edu.my/id/eprint/113864/ http://psasir.upm.edu.my/id/eprint/113864/ http://psasir.upm.edu.my/id/eprint/113864/ |