Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs)
Meniscus-derived stem cells (MeSCs), a unique type of MSC, have outstanding advantages in meniscal cytotherapy and tissue engineering, but the effects and molecular mechanisms of PBM on MeSCs are still unclear. We used 660-nm LED light with different energy densities to irradiate six human MeSC samp...
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| Format: | Article |
| Language: | English |
| Published: |
Nature Research
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113843/ http://psasir.upm.edu.my/id/eprint/113843/1/113843.pdf |
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| author | Tong, Jiabei Subbiah, Suresh Kumar Rampal, Sanjiv Ramasamy, Rajesh Wu, Xiaoyun You, Yanyan Wang, Jiaojiao Mok, Pooi Ling |
| author_facet | Tong, Jiabei Subbiah, Suresh Kumar Rampal, Sanjiv Ramasamy, Rajesh Wu, Xiaoyun You, Yanyan Wang, Jiaojiao Mok, Pooi Ling |
| author_sort | Tong, Jiabei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Meniscus-derived stem cells (MeSCs), a unique type of MSC, have outstanding advantages in meniscal cytotherapy and tissue engineering, but the effects and molecular mechanisms of PBM on MeSCs are still unclear. We used 660-nm LED light with different energy densities to irradiate six human MeSC samples and tested their proliferation rate via cell counting, chondrogenic differentiation capacity via the DMMB assay, mitochondrial activity via the MTT assay, and gene expression via qPCR. The proliferation ability, chondrogenic capacity and mitochondrial activity of the 18 J/cm2 group were greater than those of the 4 J/cm2 and control groups. The mRNA expression levels of Akt, PI3K, TGF-β3, Ki67 and Notch-1 in the 18 J/cm2 group were greater than those in the other groups in most samples. After chondrogenic induction, the expression of Col2A1, Sox9 and Aggrecan in the 18 J/cm2 group was significantly greater than that in the 4 J/cm2 and control groups in most of the samples. The variation in the MTT values and Src, PI3K, Akt, mTOR and GSK3β levels decreased with time. The results showed that 660-nm LED red light promoted proliferation and chondrogenic differentiation and affected the gene expression of MeSCs, and the effects on gene expression and mitochondrial activity decreased with time. |
| first_indexed | 2025-11-15T14:19:00Z |
| format | Article |
| id | upm-113843 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:19:00Z |
| publishDate | 2024 |
| publisher | Nature Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1138432025-01-13T06:42:18Z http://psasir.upm.edu.my/id/eprint/113843/ Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs) Tong, Jiabei Subbiah, Suresh Kumar Rampal, Sanjiv Ramasamy, Rajesh Wu, Xiaoyun You, Yanyan Wang, Jiaojiao Mok, Pooi Ling Meniscus-derived stem cells (MeSCs), a unique type of MSC, have outstanding advantages in meniscal cytotherapy and tissue engineering, but the effects and molecular mechanisms of PBM on MeSCs are still unclear. We used 660-nm LED light with different energy densities to irradiate six human MeSC samples and tested their proliferation rate via cell counting, chondrogenic differentiation capacity via the DMMB assay, mitochondrial activity via the MTT assay, and gene expression via qPCR. The proliferation ability, chondrogenic capacity and mitochondrial activity of the 18 J/cm2 group were greater than those of the 4 J/cm2 and control groups. The mRNA expression levels of Akt, PI3K, TGF-β3, Ki67 and Notch-1 in the 18 J/cm2 group were greater than those in the other groups in most samples. After chondrogenic induction, the expression of Col2A1, Sox9 and Aggrecan in the 18 J/cm2 group was significantly greater than that in the 4 J/cm2 and control groups in most of the samples. The variation in the MTT values and Src, PI3K, Akt, mTOR and GSK3β levels decreased with time. The results showed that 660-nm LED red light promoted proliferation and chondrogenic differentiation and affected the gene expression of MeSCs, and the effects on gene expression and mitochondrial activity decreased with time. Nature Research 2024-08-26 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/113843/1/113843.pdf Tong, Jiabei and Subbiah, Suresh Kumar and Rampal, Sanjiv and Ramasamy, Rajesh and Wu, Xiaoyun and You, Yanyan and Wang, Jiaojiao and Mok, Pooi Ling (2024) Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs). Scientific Reports, 14 (1). art. no. 19735. ISSN 2045-2322; eISSN: 2045-2322 https://www.nature.com/articles/s41598-024-70258-0?error=cookies_not_supported&code=5392ebbb-7e56-4b04-bef2-d3e2e786b728 10.1038/s41598-024-70258-0 |
| spellingShingle | Tong, Jiabei Subbiah, Suresh Kumar Rampal, Sanjiv Ramasamy, Rajesh Wu, Xiaoyun You, Yanyan Wang, Jiaojiao Mok, Pooi Ling Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs) |
| title | Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs) |
| title_full | Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs) |
| title_fullStr | Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs) |
| title_full_unstemmed | Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs) |
| title_short | Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs) |
| title_sort | effect of 660-nm led photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (mescs) |
| url | http://psasir.upm.edu.my/id/eprint/113843/ http://psasir.upm.edu.my/id/eprint/113843/ http://psasir.upm.edu.my/id/eprint/113843/ http://psasir.upm.edu.my/id/eprint/113843/1/113843.pdf |