Identification of novel functional compounds from forest onion and its biological activities against breast cancer
The discovery of new molecules from natural sources for the treatment of tumors such as breast cancers is of importance for the development of functional foods and to the pharmaceutical industry. A natural resource with potential activity against breast cancer is forest onion, Eleutherine bulbosa (M...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113755/ http://psasir.upm.edu.my/id/eprint/113755/1/113755.pdf |
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| author | Nurkolis, Fahrul Kurniatanty, Isma Wiyarta, Elvan Permatasari, Happy Kurnia Mayulu, Nelly Taslim, Nurpudji Astuti Tjandrawinata, Raymond Rubianto Hardinsyah, Hardinsyah Tallei, Trina Ekawati Tsopmo, Apollinaire Radu, Son Hadinata, Edwin Kim, Bonglee Ribeiro, Rosy Iara Maciel Azambuja Syahputra, Rony Abdi |
| author_facet | Nurkolis, Fahrul Kurniatanty, Isma Wiyarta, Elvan Permatasari, Happy Kurnia Mayulu, Nelly Taslim, Nurpudji Astuti Tjandrawinata, Raymond Rubianto Hardinsyah, Hardinsyah Tallei, Trina Ekawati Tsopmo, Apollinaire Radu, Son Hadinata, Edwin Kim, Bonglee Ribeiro, Rosy Iara Maciel Azambuja Syahputra, Rony Abdi |
| author_sort | Nurkolis, Fahrul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The discovery of new molecules from natural sources for the treatment of tumors such as breast cancers is of importance for the development of functional foods and to the pharmaceutical industry. A natural resource with potential activity against breast cancer is forest onion, Eleutherine bulbosa (Mill.) Urb., but the identity of its active constituents and their mechanisms of action remain unexplored. Therefore, this study focuses on metabolite profiling, in silico or pharmacoinformatic activity and mechanisms, as well as advanced validation on in vitro cell lines. Ten compounds identified in E. bulbosa bulb ethanolic extract (EBE) showed cancer receptor and radical inhibitory activity via network pharmacology and molecular docking simulation. The most promising compound was avenasterol binding PARP-1, HER2, iNOS receptors with values of −11.26, −8.34, and −9.17 μg/mL, respectively. EBE and avenasterol had a smaller EC50 value, or higher potency, than the control antioxidant Trolox in radical scavenging tests with ABTS and DPPH. In line with the in silico study, EBE and avenasterol showed antiproliferative activity against human breast cancer MCF-7 with LD50 217.8 μg/mL, with relatively low cytotoxicity to normal MCF-10A cells (LD50 > 1000 μg/mL). The antiproliferative mechanism of EBE on MCF-7 was associated with downregulation of TGF-β, HER2, PI3K, and AKT which are known tumor activators. Significant (p < 0.05) upregulation of tumor suppressor gene miR-29a-3p in MCF-7 was observed after treatment with EBE in a dose-dependent manner. © 2024 The Authors |
| first_indexed | 2025-11-15T14:18:37Z |
| format | Article |
| id | upm-113755 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:18:37Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1137552025-01-14T02:11:05Z http://psasir.upm.edu.my/id/eprint/113755/ Identification of novel functional compounds from forest onion and its biological activities against breast cancer Nurkolis, Fahrul Kurniatanty, Isma Wiyarta, Elvan Permatasari, Happy Kurnia Mayulu, Nelly Taslim, Nurpudji Astuti Tjandrawinata, Raymond Rubianto Hardinsyah, Hardinsyah Tallei, Trina Ekawati Tsopmo, Apollinaire Radu, Son Hadinata, Edwin Kim, Bonglee Ribeiro, Rosy Iara Maciel Azambuja Syahputra, Rony Abdi The discovery of new molecules from natural sources for the treatment of tumors such as breast cancers is of importance for the development of functional foods and to the pharmaceutical industry. A natural resource with potential activity against breast cancer is forest onion, Eleutherine bulbosa (Mill.) Urb., but the identity of its active constituents and their mechanisms of action remain unexplored. Therefore, this study focuses on metabolite profiling, in silico or pharmacoinformatic activity and mechanisms, as well as advanced validation on in vitro cell lines. Ten compounds identified in E. bulbosa bulb ethanolic extract (EBE) showed cancer receptor and radical inhibitory activity via network pharmacology and molecular docking simulation. The most promising compound was avenasterol binding PARP-1, HER2, iNOS receptors with values of −11.26, −8.34, and −9.17 μg/mL, respectively. EBE and avenasterol had a smaller EC50 value, or higher potency, than the control antioxidant Trolox in radical scavenging tests with ABTS and DPPH. In line with the in silico study, EBE and avenasterol showed antiproliferative activity against human breast cancer MCF-7 with LD50 217.8 μg/mL, with relatively low cytotoxicity to normal MCF-10A cells (LD50 > 1000 μg/mL). The antiproliferative mechanism of EBE on MCF-7 was associated with downregulation of TGF-β, HER2, PI3K, and AKT which are known tumor activators. Significant (p < 0.05) upregulation of tumor suppressor gene miR-29a-3p in MCF-7 was observed after treatment with EBE in a dose-dependent manner. © 2024 The Authors Elsevier 2024 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/113755/1/113755.pdf Nurkolis, Fahrul and Kurniatanty, Isma and Wiyarta, Elvan and Permatasari, Happy Kurnia and Mayulu, Nelly and Taslim, Nurpudji Astuti and Tjandrawinata, Raymond Rubianto and Hardinsyah, Hardinsyah and Tallei, Trina Ekawati and Tsopmo, Apollinaire and Radu, Son and Hadinata, Edwin and Kim, Bonglee and Ribeiro, Rosy Iara Maciel Azambuja and Syahputra, Rony Abdi (2024) Identification of novel functional compounds from forest onion and its biological activities against breast cancer. Journal of Agriculture and Food Research, 18. art. no. 101362. pp. 1-14. ISSN 2666-1543; eISSN: 2666-1543 https://linkinghub.elsevier.com/retrieve/pii/S2666154324003995 10.1016/j.jafr.2024.101362 |
| spellingShingle | Nurkolis, Fahrul Kurniatanty, Isma Wiyarta, Elvan Permatasari, Happy Kurnia Mayulu, Nelly Taslim, Nurpudji Astuti Tjandrawinata, Raymond Rubianto Hardinsyah, Hardinsyah Tallei, Trina Ekawati Tsopmo, Apollinaire Radu, Son Hadinata, Edwin Kim, Bonglee Ribeiro, Rosy Iara Maciel Azambuja Syahputra, Rony Abdi Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_full | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_fullStr | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_full_unstemmed | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_short | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_sort | identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| url | http://psasir.upm.edu.my/id/eprint/113755/ http://psasir.upm.edu.my/id/eprint/113755/ http://psasir.upm.edu.my/id/eprint/113755/ http://psasir.upm.edu.my/id/eprint/113755/1/113755.pdf |