Plant regeneration of Michelia champaca L., through somatic embryogenesis
Michelia champaca L. is a woody ornamental tree species which has high commercial value to be used as a basic material for perfume, cosmetic, and medicine. The development of an efficient plant regeneration system for M. champaca is essential for the production of Champaca planting material and prec...
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| Format: | Article |
| Language: | English |
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Academic Journals
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11373/ http://psasir.upm.edu.my/id/eprint/11373/1/Plant_regeneration_of_Michelia_champaca_L.%2C_through.pdf |
| _version_ | 1848841622680240128 |
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| author | Armiyanti, Abdul Kadir, Mihdzar Kadzimin, Saleh Panjaitan, Syaiful Bahri |
| author_facet | Armiyanti, Abdul Kadir, Mihdzar Kadzimin, Saleh Panjaitan, Syaiful Bahri |
| author_sort | Armiyanti, |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Michelia champaca L. is a woody ornamental tree species which has high commercial value to be used as a basic material for perfume, cosmetic, and medicine. The development of an efficient plant regeneration system for M. champaca is essential for the production of Champaca planting material and precondition for genetic manipulation. Plant regeneration systems of M. champacathrough somatic embryogenesis derived from immature seed of M. champaca L. which was successfully developed in this study. MS medium supplemented with 2 mgL-1, NAA produced highest percentage of embryogenic callus formation of (43%). The embryogenic cells proliferated and formed somatic embryos (30%) after four to six months of culture in the same medium. Meanwhile, on germination of somatic embryos, hormone free of MS medium resulted in highest percentage of normal plantlets produced (45%) compared with other germination medium which is containing different GA3 concentrations with ranges from 1 - 8% somatic embryos germinated. |
| first_indexed | 2025-11-15T07:46:10Z |
| format | Article |
| id | upm-11373 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:46:10Z |
| publishDate | 2010 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-113732016-06-30T08:57:15Z http://psasir.upm.edu.my/id/eprint/11373/ Plant regeneration of Michelia champaca L., through somatic embryogenesis Armiyanti, Abdul Kadir, Mihdzar Kadzimin, Saleh Panjaitan, Syaiful Bahri Michelia champaca L. is a woody ornamental tree species which has high commercial value to be used as a basic material for perfume, cosmetic, and medicine. The development of an efficient plant regeneration system for M. champaca is essential for the production of Champaca planting material and precondition for genetic manipulation. Plant regeneration systems of M. champacathrough somatic embryogenesis derived from immature seed of M. champaca L. which was successfully developed in this study. MS medium supplemented with 2 mgL-1, NAA produced highest percentage of embryogenic callus formation of (43%). The embryogenic cells proliferated and formed somatic embryos (30%) after four to six months of culture in the same medium. Meanwhile, on germination of somatic embryos, hormone free of MS medium resulted in highest percentage of normal plantlets produced (45%) compared with other germination medium which is containing different GA3 concentrations with ranges from 1 - 8% somatic embryos germinated. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11373/1/Plant_regeneration_of_Michelia_champaca_L.%2C_through.pdf Armiyanti, and Abdul Kadir, Mihdzar and Kadzimin, Saleh and Panjaitan, Syaiful Bahri (2010) Plant regeneration of Michelia champaca L., through somatic embryogenesis. African Journal of Biotechnology, 9 (18). art. no. C5F75FC17976. pp. 2640-2647. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/C5F75FC17976 |
| spellingShingle | Armiyanti, Abdul Kadir, Mihdzar Kadzimin, Saleh Panjaitan, Syaiful Bahri Plant regeneration of Michelia champaca L., through somatic embryogenesis |
| title | Plant regeneration of Michelia champaca L., through somatic embryogenesis |
| title_full | Plant regeneration of Michelia champaca L., through somatic embryogenesis |
| title_fullStr | Plant regeneration of Michelia champaca L., through somatic embryogenesis |
| title_full_unstemmed | Plant regeneration of Michelia champaca L., through somatic embryogenesis |
| title_short | Plant regeneration of Michelia champaca L., through somatic embryogenesis |
| title_sort | plant regeneration of michelia champaca l., through somatic embryogenesis |
| url | http://psasir.upm.edu.my/id/eprint/11373/ http://psasir.upm.edu.my/id/eprint/11373/ http://psasir.upm.edu.my/id/eprint/11373/1/Plant_regeneration_of_Michelia_champaca_L.%2C_through.pdf |