Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm- like bodies by vitrification
Protocorm-like bodies of Dendrobium Bobby Messina were cryopreserved by vitrification method. In this study, protocorm-like bodies (PLBs) with the size range of 1-2 and 3-4 mm were selected from 4 weeks old culture, pretreated with half strength semi-solid Murashige and Skoog (MS) media supplemented...
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| Format: | Article |
| Language: | English |
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Academic Journals
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11372/ http://psasir.upm.edu.my/id/eprint/11372/1/Preliminary_Study_of_Cryopreservation_of_Dendrobium_Bobby_Messina_Protocorm-like_Bodies_by_Vitrification.pdf |
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| author | James Antony, Jessica Jeyanthi Chan, Lai Keng Rathinam, Xavier Sinniah, Uma Rani Subramaniam, Sreeramanan |
| author_facet | James Antony, Jessica Jeyanthi Chan, Lai Keng Rathinam, Xavier Sinniah, Uma Rani Subramaniam, Sreeramanan |
| author_sort | James Antony, Jessica Jeyanthi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Protocorm-like bodies of Dendrobium Bobby Messina were cryopreserved by vitrification method. In this study, protocorm-like bodies (PLBs) with the size range of 1-2 and 3-4 mm were selected from 4 weeks old culture, pretreated with half strength semi-solid Murashige and Skoog (MS) media supplemented with 0.5 M sucrose at 25°C for 24 h. Pretreated PLBs were then treated with a mixture of 2 M glycerol and 0.4 M sucrose supplemented with half strength liquid MS media at 25°C for 20 min. Osmoprotected PLBs were then dehydrated with plant vitrification solution 2 at 0°C for 20 min before storage in liquid nitrogen. After rapid warming in water bath at 40°C for 90 s, the PLBs were washed with half strength liquid MS media supplemented with 1.2 M sucrose and then cultured on half strength semi-solid MS media supplemented with 2% sucrose without the presence of any growth regulators. Survival of the cryopreserved PLBs was assessed based on triphenyl tetrazoliumchloride (TTC) spectrophotometrical analysis. The PLBs with 3-4 mm size range showed better viability comparative to size range 1-2 mm for both cryopreserved and non-cryopreserved PLBs. The best pretreatment concentration used in pretreatment media was 0.6 M sucrose and 1.2 M sorbitol, respectively. |
| first_indexed | 2025-11-15T07:46:10Z |
| format | Article |
| id | upm-11372 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:46:10Z |
| publishDate | 2010 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-113722017-11-15T09:37:34Z http://psasir.upm.edu.my/id/eprint/11372/ Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm- like bodies by vitrification James Antony, Jessica Jeyanthi Chan, Lai Keng Rathinam, Xavier Sinniah, Uma Rani Subramaniam, Sreeramanan Protocorm-like bodies of Dendrobium Bobby Messina were cryopreserved by vitrification method. In this study, protocorm-like bodies (PLBs) with the size range of 1-2 and 3-4 mm were selected from 4 weeks old culture, pretreated with half strength semi-solid Murashige and Skoog (MS) media supplemented with 0.5 M sucrose at 25°C for 24 h. Pretreated PLBs were then treated with a mixture of 2 M glycerol and 0.4 M sucrose supplemented with half strength liquid MS media at 25°C for 20 min. Osmoprotected PLBs were then dehydrated with plant vitrification solution 2 at 0°C for 20 min before storage in liquid nitrogen. After rapid warming in water bath at 40°C for 90 s, the PLBs were washed with half strength liquid MS media supplemented with 1.2 M sucrose and then cultured on half strength semi-solid MS media supplemented with 2% sucrose without the presence of any growth regulators. Survival of the cryopreserved PLBs was assessed based on triphenyl tetrazoliumchloride (TTC) spectrophotometrical analysis. The PLBs with 3-4 mm size range showed better viability comparative to size range 1-2 mm for both cryopreserved and non-cryopreserved PLBs. The best pretreatment concentration used in pretreatment media was 0.6 M sucrose and 1.2 M sorbitol, respectively. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11372/1/Preliminary_Study_of_Cryopreservation_of_Dendrobium_Bobby_Messina_Protocorm-like_Bodies_by_Vitrification.pdf James Antony, Jessica Jeyanthi and Chan, Lai Keng and Rathinam, Xavier and Sinniah, Uma Rani and Subramaniam, Sreeramanan (2010) Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm- like bodies by vitrification. African Journal of Biotechnology, 9 (42). art. no. 358261823850. pp. 7063-7070. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/358261823850 |
| spellingShingle | James Antony, Jessica Jeyanthi Chan, Lai Keng Rathinam, Xavier Sinniah, Uma Rani Subramaniam, Sreeramanan Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm- like bodies by vitrification |
| title | Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm- like bodies by vitrification |
| title_full | Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm- like bodies by vitrification |
| title_fullStr | Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm- like bodies by vitrification |
| title_full_unstemmed | Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm- like bodies by vitrification |
| title_short | Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm- like bodies by vitrification |
| title_sort | preliminary study on cryopreservation of dendrobium bobby messina protocorm- like bodies by vitrification |
| url | http://psasir.upm.edu.my/id/eprint/11372/ http://psasir.upm.edu.my/id/eprint/11372/ http://psasir.upm.edu.my/id/eprint/11372/1/Preliminary_Study_of_Cryopreservation_of_Dendrobium_Bobby_Messina_Protocorm-like_Bodies_by_Vitrification.pdf |