Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging
Aims: This study aims to evaluate the storage stability of the freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder expressing K-ras (Kristen rat sarcoma viral oncogene homolog) mimotopes targeting colorectal cancer in vacuum packaging. Methods and results: The freeze-dried L. la...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113631/ http://psasir.upm.edu.my/id/eprint/113631/1/113631.pdf |
| _version_ | 1848866280752283648 |
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| author | How, Yu Hsuan Teo, Michelle Yee Mun In, Lionel Lian Aun Yeo, Siok Koon Bhandari, Bhesh Yusof, Yus Aniza Pui, Liew Phing |
| author_facet | How, Yu Hsuan Teo, Michelle Yee Mun In, Lionel Lian Aun Yeo, Siok Koon Bhandari, Bhesh Yusof, Yus Aniza Pui, Liew Phing |
| author_sort | How, Yu Hsuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aims: This study aims to evaluate the storage stability of the freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder expressing K-ras (Kristen rat sarcoma viral oncogene homolog) mimotopes targeting colorectal cancer in vacuum packaging. Methods and results: The freeze-dried L. lactis-fermented milk powder stored in 4-ply retortable polypropylene (RCPP)-polyamide (PA)-Aluminium (AL)-polyethylene terephthalate (PET) and aluminium polyethylene (ALPE) was evaluated throughout 49 days of accelerated storage (38°C and 90% relative humidity). The fermented milk powder stored in 4-ply packaging remained above 6 log10 CFU g-1 viability, displayed lower moisture content (6.1%), higher flowability (43° angle of repose), water solubility (62%), and survivability of L. lactis after simulated gastric and intestinal digestion (>82%) than ALPE packaging after 42 days of accelerated storage. K-ras mimotope expression was detected intracellularly and extracellularly in the freeze-dried L. lactis-fermented milk powder upon storage. Conclusions: This suggests that fermented milk powder is a suitable food carrier for this live oral vaccine. |
| first_indexed | 2025-11-15T14:18:06Z |
| format | Article |
| id | upm-113631 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:18:06Z |
| publishDate | 2024 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1136312024-11-14T02:58:37Z http://psasir.upm.edu.my/id/eprint/113631/ Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging How, Yu Hsuan Teo, Michelle Yee Mun In, Lionel Lian Aun Yeo, Siok Koon Bhandari, Bhesh Yusof, Yus Aniza Pui, Liew Phing Aims: This study aims to evaluate the storage stability of the freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder expressing K-ras (Kristen rat sarcoma viral oncogene homolog) mimotopes targeting colorectal cancer in vacuum packaging. Methods and results: The freeze-dried L. lactis-fermented milk powder stored in 4-ply retortable polypropylene (RCPP)-polyamide (PA)-Aluminium (AL)-polyethylene terephthalate (PET) and aluminium polyethylene (ALPE) was evaluated throughout 49 days of accelerated storage (38°C and 90% relative humidity). The fermented milk powder stored in 4-ply packaging remained above 6 log10 CFU g-1 viability, displayed lower moisture content (6.1%), higher flowability (43° angle of repose), water solubility (62%), and survivability of L. lactis after simulated gastric and intestinal digestion (>82%) than ALPE packaging after 42 days of accelerated storage. K-ras mimotope expression was detected intracellularly and extracellularly in the freeze-dried L. lactis-fermented milk powder upon storage. Conclusions: This suggests that fermented milk powder is a suitable food carrier for this live oral vaccine. Oxford University Press 2024 Article PeerReviewed text en cc_by_nc_4 http://psasir.upm.edu.my/id/eprint/113631/1/113631.pdf How, Yu Hsuan and Teo, Michelle Yee Mun and In, Lionel Lian Aun and Yeo, Siok Koon and Bhandari, Bhesh and Yusof, Yus Aniza and Pui, Liew Phing (2024) Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging. Journal of Applied Microbiology, 135 (7). art. no. lxae162. pp. 1-18. ISSN 1364-5072; eISSN: 1365-2672 https://academic.oup.com/jambio/article/135/7/lxae162/7704451?login=true 10.1093/jambio/lxae162 |
| spellingShingle | How, Yu Hsuan Teo, Michelle Yee Mun In, Lionel Lian Aun Yeo, Siok Koon Bhandari, Bhesh Yusof, Yus Aniza Pui, Liew Phing Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging |
| title | Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging |
| title_full | Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging |
| title_fullStr | Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging |
| title_full_unstemmed | Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging |
| title_short | Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging |
| title_sort | stability and expression of k-ras mimotopes in freeze-dried recombinant lactococcus lactis nz3900-fermented milk powder during storage in vacuum packaging |
| url | http://psasir.upm.edu.my/id/eprint/113631/ http://psasir.upm.edu.my/id/eprint/113631/ http://psasir.upm.edu.my/id/eprint/113631/ http://psasir.upm.edu.my/id/eprint/113631/1/113631.pdf |