Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells
Matrix metallopeptidase 3 (MMP3) is among the key players in breast cancer metastasis that contributes to the highest cancer-related deaths in females globally. Previously, in silico analyses had shown that several coding single nucleotide polymorphisms (SNPs) of MMP3 were predicted to alter the sec...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113612/ http://psasir.upm.edu.my/id/eprint/113612/1/113612.pdf |
| _version_ | 1848866274452439040 |
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| author | Ahmad Suhaimi, Shafinah Soon, Choy Chan Chong, Pei Pei Saad, Norazalina Chau, De Ming Rosli, Rozita |
| author_facet | Ahmad Suhaimi, Shafinah Soon, Choy Chan Chong, Pei Pei Saad, Norazalina Chau, De Ming Rosli, Rozita |
| author_sort | Ahmad Suhaimi, Shafinah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Matrix metallopeptidase 3 (MMP3) is among the key players in breast cancer metastasis that contributes to the highest cancer-related deaths in females globally. Previously, in silico analyses had shown that several coding single nucleotide polymorphisms (SNPs) of MMP3 were predicted to alter the secondary structures of MMP3 and subsequently reduce its mRNA stability. To validate the mentioned hypotheses, this study aimed to determine the effects of six coding SNPs of MMP3 on its mRNA stability, protein expression level as well as cell invasiveness in vitro. In this study, breast adenocarcinoma MCF7 cells were transfected with MMP3 wild type (MMP3-WT) and a variant containing SNPs (MMP3-Var). Following transfection, protein expression level, mRNA stability and enzyme activity of MMP3-WT and MMP3-Var were evaluated. Finally, the effect of MMP3 coding SNPs on cell invasiveness in breast cancer was determined. In this study, the mRNA stability, protein expression level and enzymatic activity of MMP3-Var were significantly reduced. Moreover, the presence of MMP3 coding SNPs led to attenuated invasiveness of transfected MCF7 cells. In conclusion, these findings may contribute to the current understanding of these coding SNPs with metastasis in breast cancer. |
| first_indexed | 2025-11-15T14:18:00Z |
| format | Article |
| id | upm-113612 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:18:00Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1136122024-11-14T03:33:21Z http://psasir.upm.edu.my/id/eprint/113612/ Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells Ahmad Suhaimi, Shafinah Soon, Choy Chan Chong, Pei Pei Saad, Norazalina Chau, De Ming Rosli, Rozita Matrix metallopeptidase 3 (MMP3) is among the key players in breast cancer metastasis that contributes to the highest cancer-related deaths in females globally. Previously, in silico analyses had shown that several coding single nucleotide polymorphisms (SNPs) of MMP3 were predicted to alter the secondary structures of MMP3 and subsequently reduce its mRNA stability. To validate the mentioned hypotheses, this study aimed to determine the effects of six coding SNPs of MMP3 on its mRNA stability, protein expression level as well as cell invasiveness in vitro. In this study, breast adenocarcinoma MCF7 cells were transfected with MMP3 wild type (MMP3-WT) and a variant containing SNPs (MMP3-Var). Following transfection, protein expression level, mRNA stability and enzyme activity of MMP3-WT and MMP3-Var were evaluated. Finally, the effect of MMP3 coding SNPs on cell invasiveness in breast cancer was determined. In this study, the mRNA stability, protein expression level and enzymatic activity of MMP3-Var were significantly reduced. Moreover, the presence of MMP3 coding SNPs led to attenuated invasiveness of transfected MCF7 cells. In conclusion, these findings may contribute to the current understanding of these coding SNPs with metastasis in breast cancer. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/113612/1/113612.pdf Ahmad Suhaimi, Shafinah and Soon, Choy Chan and Chong, Pei Pei and Saad, Norazalina and Chau, De Ming and Rosli, Rozita (2024) Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells. Sains Malaysiana, 53 (6). art. no. undefined. pp. 1243-1253. ISSN 0126-6039 https://www.ukm.my/jsm/pdf_files/SM-PDF-53-6-2024/2.pdf 10.17576/jsm-2024-5306-02 |
| spellingShingle | Ahmad Suhaimi, Shafinah Soon, Choy Chan Chong, Pei Pei Saad, Norazalina Chau, De Ming Rosli, Rozita Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_full | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_fullStr | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_full_unstemmed | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_short | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_sort | matrix metallopeptidase 3 coding snps suppress cell invasion in mcf7 breast cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/113612/ http://psasir.upm.edu.my/id/eprint/113612/ http://psasir.upm.edu.my/id/eprint/113612/ http://psasir.upm.edu.my/id/eprint/113612/1/113612.pdf |