Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation
Bacterial nanocellulose (BNC) is a remarkable biopolymer synthesised by bacterium, exhibiting exceptional properties. However, conventional Hestrin-Schramm (HS) medium, particularly the carbon source, poses challenges of high costs and low productivity. This study explores BNC biosynthesis on a modi...
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| Format: | Article |
| Language: | English |
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University of Malaya
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113411/ http://psasir.upm.edu.my/id/eprint/113411/1/113411.pdf |
| _version_ | 1848866215344209920 |
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| author | Daud, Aini Darwina Abdul Rahman, Nor’Aini Foo, Hooi Ling Mohamad, Rosfarizan |
| author_facet | Daud, Aini Darwina Abdul Rahman, Nor’Aini Foo, Hooi Ling Mohamad, Rosfarizan |
| author_sort | Daud, Aini Darwina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bacterial nanocellulose (BNC) is a remarkable biopolymer synthesised by bacterium, exhibiting exceptional properties. However, conventional Hestrin-Schramm (HS) medium, particularly the carbon source, poses challenges of high costs and low productivity. This study explores BNC biosynthesis on a modified HS medium, employing agricultural wastes (sugarcane molasses, banana peel, and pineapple peel) as carbon sources, and compares the overall yield of BNC produced. Sugarcane molasses proved to be the most effective, yielding the highest BNC concentration (8.19 g/L) after 7 days, followed by pineapple peel (2.16 g/L) and banana peel (2.11 g/L). Extensive research was conducted to enhance properties of BNC by an environmentally friendly approach, incorporating silver nanoparticles (AgNP) utilising Momordica charantia fruit extract, resulting in a BNC-Ag nanocomposite. The synthesis involved mixing 1 mM silver nitrate (AgNO3) with 15 mL of M. charantia fruit extract to reduce Ag ions to AgNP, which was confirmed by UV-vis spectroscopy with an absorbance peak between 400 and 410 nm. Characterisation using FESEM and TEM on the synthesized BNC showed minimal impact on BNC fiber diameter from waste-derived carbon sources. XRD indicated slight variations in crystallinity index, with the highest (85%) in TSM-derived BNC. FTIR analysis revealed similar chemical profiles across all BNC, indicating cellulose formation. The BNC-Ag nanocomposite exhibited potent antibacterial activity against multi-drug resistant strains (Pseudomonas aeruginosa, Salmonella typhi, Bacillus subtilis, Staphylococcus aureus) through disc diffusion method with inhibition zones up to 16.8 mm. Overall, the findings from this study contribute to the development of environmentally sustainable for the production of functional BNC materials with enhanced properties for diverse applications. |
| first_indexed | 2025-11-15T14:17:03Z |
| format | Article |
| id | upm-113411 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:17:03Z |
| publishDate | 2024 |
| publisher | University of Malaya |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1134112024-11-22T08:32:52Z http://psasir.upm.edu.my/id/eprint/113411/ Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation Daud, Aini Darwina Abdul Rahman, Nor’Aini Foo, Hooi Ling Mohamad, Rosfarizan Bacterial nanocellulose (BNC) is a remarkable biopolymer synthesised by bacterium, exhibiting exceptional properties. However, conventional Hestrin-Schramm (HS) medium, particularly the carbon source, poses challenges of high costs and low productivity. This study explores BNC biosynthesis on a modified HS medium, employing agricultural wastes (sugarcane molasses, banana peel, and pineapple peel) as carbon sources, and compares the overall yield of BNC produced. Sugarcane molasses proved to be the most effective, yielding the highest BNC concentration (8.19 g/L) after 7 days, followed by pineapple peel (2.16 g/L) and banana peel (2.11 g/L). Extensive research was conducted to enhance properties of BNC by an environmentally friendly approach, incorporating silver nanoparticles (AgNP) utilising Momordica charantia fruit extract, resulting in a BNC-Ag nanocomposite. The synthesis involved mixing 1 mM silver nitrate (AgNO3) with 15 mL of M. charantia fruit extract to reduce Ag ions to AgNP, which was confirmed by UV-vis spectroscopy with an absorbance peak between 400 and 410 nm. Characterisation using FESEM and TEM on the synthesized BNC showed minimal impact on BNC fiber diameter from waste-derived carbon sources. XRD indicated slight variations in crystallinity index, with the highest (85%) in TSM-derived BNC. FTIR analysis revealed similar chemical profiles across all BNC, indicating cellulose formation. The BNC-Ag nanocomposite exhibited potent antibacterial activity against multi-drug resistant strains (Pseudomonas aeruginosa, Salmonella typhi, Bacillus subtilis, Staphylococcus aureus) through disc diffusion method with inhibition zones up to 16.8 mm. Overall, the findings from this study contribute to the development of environmentally sustainable for the production of functional BNC materials with enhanced properties for diverse applications. University of Malaya 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/113411/1/113411.pdf Daud, Aini Darwina and Abdul Rahman, Nor’Aini and Foo, Hooi Ling and Mohamad, Rosfarizan (2024) Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation. Asia-Pacific Journal of Molecular Biology and Biotechnology, 32 (2). pp. 1-14. ISSN 0128-7451; eISSN: 0128-7451 https://www.msmbb.my/images/publication/volume_32/issue_2/01-Daud-et-al.pdf 10.35118/apjmbb.2024.032.2.01 |
| spellingShingle | Daud, Aini Darwina Abdul Rahman, Nor’Aini Foo, Hooi Ling Mohamad, Rosfarizan Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation |
| title | Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation |
| title_full | Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation |
| title_fullStr | Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation |
| title_full_unstemmed | Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation |
| title_short | Bacterial nanocellulose (BNC) biosynthesis by Komagataeibacter hansenii RM-03 using agricultural waste as substrates and BNC-silver nanocomposite preparation |
| title_sort | bacterial nanocellulose (bnc) biosynthesis by komagataeibacter hansenii rm-03 using agricultural waste as substrates and bnc-silver nanocomposite preparation |
| url | http://psasir.upm.edu.my/id/eprint/113411/ http://psasir.upm.edu.my/id/eprint/113411/ http://psasir.upm.edu.my/id/eprint/113411/ http://psasir.upm.edu.my/id/eprint/113411/1/113411.pdf |