Assessment of heavy metals removal using bioflocculant produced by Bacillus subtilis UPMB10
Due to urbanization, industrialization, and agricultural activities, heavy metal pollution has become one of the most significant environmental issues. Heavy metals are highly soluble in the aquatic environment, which makes it possible for them to bio-accumulate and magnify in living organisms, l...
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| Format: | Thesis |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/113134/ http://psasir.upm.edu.my/id/eprint/113134/1/113134.pdf |
| _version_ | 1848866137530433536 |
|---|---|
| author | Wang, Yang |
| author_facet | Wang, Yang |
| author_sort | Wang, Yang |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Due to urbanization, industrialization, and agricultural activities, heavy metal
pollution has become one of the most significant environmental issues. Heavy
metals are highly soluble in the aquatic environment, which makes it possible for
them to bio-accumulate and magnify in living organisms, leading to health
issues. Therefore, metal-contaminated wastewater must be treated before being
released into the environment. Conventional methods for metal removal are
expensive and generate secondary pollution. Bioflocculants have received more
attention as they can be an alternative solution to remove heavy metals because
of their safety and biodegradability. The heavy metal removal efficiency of
bioflocculant produced by Bacillus subtilis UPMB10 for three metals, zinc (Zn),
arsenic (As) and lead (Pb) in single and mixed synthetic metal solutions and exmining
lake water collected from Kg. Gajah, Perak was studied. One-factor-ata-
time (OFAT) analysis was used to assess the influence of bioflocculant
dosage, initial pH, and initial metal concentration on the efficiency of heavy metal
removal. The concentration of metals before and after treatment was measured
by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). The
results showed that at 5 mg/L of metal concentration and pH 7, the removal
efficiency of Zn and As significantly increased with an increment in bioflocculant
dosage from 5% (v/v) to 20% (v/v) (p ≤ 0.05). However, the removal efficiency
of Pb significantly declined with increased bioflocculant dosages (p ≤ 0.05). In
the 5 mg/L of metal concentration with the bioflocculant dosages of 5% (v/v),
heavy metals (Zn, As, Pb) showed the highest removal efficiency at pH 7 than
at pH 4 and 9 with significant difference (p ≤ 0.05). With bioflocculant dosages
of 5% (v/v) at pH 7, the efficiency to remove heavy metals (Zn, As, and Pb)
significantly increased as the heavy metal concentration increased from 1 to 60
mg/L (p ≤ 0.05). Under all conditions, Zn and As were more effectively removed
from mixed metal solution than from single metal solution. Conversely, Pb was
more effectively removed from single metal solution. In this study, the highest
removal efficiencies for Zn (66.40%) and As (45.45%) were found to occur in a
mixed metal solution of 60 mg/L with a bioflocculant dosage of 5% (v/v) and pH
7. Meanwhile, the highest Pb removal efficiency (98.74%) was found in a single
metal solution of 60 mg/L with a bioflocculant dosage of 5% (v/v) and pH 7. On
the other hand, heavy metals (Zn, As, Pb) in the ex-mining lake water samples
were effectively removed at 56.4%, 64.0%, and 78.0% with bioflocculant
dosages of 20% (v/v), respectively. Overall, bioflocculant produced by B. subtilis
UPMB10 has the potential to remove Zn, As, and Pb, and could be used as an
effective bioflocculant agent for environmental remediation of heavy metals |
| first_indexed | 2025-11-15T14:15:49Z |
| format | Thesis |
| id | upm-113134 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:15:49Z |
| publishDate | 2023 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1131342024-10-25T08:52:57Z http://psasir.upm.edu.my/id/eprint/113134/ Assessment of heavy metals removal using bioflocculant produced by Bacillus subtilis UPMB10 Wang, Yang Due to urbanization, industrialization, and agricultural activities, heavy metal pollution has become one of the most significant environmental issues. Heavy metals are highly soluble in the aquatic environment, which makes it possible for them to bio-accumulate and magnify in living organisms, leading to health issues. Therefore, metal-contaminated wastewater must be treated before being released into the environment. Conventional methods for metal removal are expensive and generate secondary pollution. Bioflocculants have received more attention as they can be an alternative solution to remove heavy metals because of their safety and biodegradability. The heavy metal removal efficiency of bioflocculant produced by Bacillus subtilis UPMB10 for three metals, zinc (Zn), arsenic (As) and lead (Pb) in single and mixed synthetic metal solutions and exmining lake water collected from Kg. Gajah, Perak was studied. One-factor-ata- time (OFAT) analysis was used to assess the influence of bioflocculant dosage, initial pH, and initial metal concentration on the efficiency of heavy metal removal. The concentration of metals before and after treatment was measured by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). The results showed that at 5 mg/L of metal concentration and pH 7, the removal efficiency of Zn and As significantly increased with an increment in bioflocculant dosage from 5% (v/v) to 20% (v/v) (p ≤ 0.05). However, the removal efficiency of Pb significantly declined with increased bioflocculant dosages (p ≤ 0.05). In the 5 mg/L of metal concentration with the bioflocculant dosages of 5% (v/v), heavy metals (Zn, As, Pb) showed the highest removal efficiency at pH 7 than at pH 4 and 9 with significant difference (p ≤ 0.05). With bioflocculant dosages of 5% (v/v) at pH 7, the efficiency to remove heavy metals (Zn, As, and Pb) significantly increased as the heavy metal concentration increased from 1 to 60 mg/L (p ≤ 0.05). Under all conditions, Zn and As were more effectively removed from mixed metal solution than from single metal solution. Conversely, Pb was more effectively removed from single metal solution. In this study, the highest removal efficiencies for Zn (66.40%) and As (45.45%) were found to occur in a mixed metal solution of 60 mg/L with a bioflocculant dosage of 5% (v/v) and pH 7. Meanwhile, the highest Pb removal efficiency (98.74%) was found in a single metal solution of 60 mg/L with a bioflocculant dosage of 5% (v/v) and pH 7. On the other hand, heavy metals (Zn, As, Pb) in the ex-mining lake water samples were effectively removed at 56.4%, 64.0%, and 78.0% with bioflocculant dosages of 20% (v/v), respectively. Overall, bioflocculant produced by B. subtilis UPMB10 has the potential to remove Zn, As, and Pb, and could be used as an effective bioflocculant agent for environmental remediation of heavy metals 2023-02 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/113134/1/113134.pdf Wang, Yang (2023) Assessment of heavy metals removal using bioflocculant produced by Bacillus subtilis UPMB10. Masters thesis, Universiti Putra Malaysia. Bacillus subtilis Flocculants Heavy metals |
| spellingShingle | Bacillus subtilis Flocculants Heavy metals Wang, Yang Assessment of heavy metals removal using bioflocculant produced by Bacillus subtilis UPMB10 |
| title | Assessment of heavy metals removal using bioflocculant produced by Bacillus subtilis UPMB10 |
| title_full | Assessment of heavy metals removal using bioflocculant produced by Bacillus subtilis UPMB10 |
| title_fullStr | Assessment of heavy metals removal using bioflocculant produced by Bacillus subtilis UPMB10 |
| title_full_unstemmed | Assessment of heavy metals removal using bioflocculant produced by Bacillus subtilis UPMB10 |
| title_short | Assessment of heavy metals removal using bioflocculant produced by Bacillus subtilis UPMB10 |
| title_sort | assessment of heavy metals removal using bioflocculant produced by bacillus subtilis upmb10 |
| topic | Bacillus subtilis Flocculants Heavy metals |
| url | http://psasir.upm.edu.my/id/eprint/113134/ http://psasir.upm.edu.my/id/eprint/113134/1/113134.pdf |