Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection
A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Sim...
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| Format: | Article |
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Springer
2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113117/ |
| _version_ | 1848866132745781248 |
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| author | Kono, Y. Yusnita, Y. Mohd Ali, A. R. Maizan, M. Sharifah, S. H. Fauzia, O. Kubo, M. Aziz, A. J. |
| author_facet | Kono, Y. Yusnita, Y. Mohd Ali, A. R. Maizan, M. Sharifah, S. H. Fauzia, O. Kubo, M. Aziz, A. J. |
| author_sort | Kono, Y. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the genus Bunyavirus was able to amplify a cDNA from Oya virus RNA. The sequence data of the product revealed that the partial gene of Oya virus S-RNA segment had 65-70% homology with published cDNA sequences of Simbu serogroup viruses. The phylogenetic analysis of the data showed that the Oya virus is grouped in Simbu serogroup, but is genetically distinct from the serogroup viruses that have been analyzed molecularly. Serological surveys revealed that the virus distributed widely and densely in Malaysia. |
| first_indexed | 2025-11-15T14:15:45Z |
| format | Article |
| id | upm-113117 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:15:45Z |
| publishDate | 2002 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1131172025-01-10T03:33:42Z http://psasir.upm.edu.my/id/eprint/113117/ Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection Kono, Y. Yusnita, Y. Mohd Ali, A. R. Maizan, M. Sharifah, S. H. Fauzia, O. Kubo, M. Aziz, A. J. A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the genus Bunyavirus was able to amplify a cDNA from Oya virus RNA. The sequence data of the product revealed that the partial gene of Oya virus S-RNA segment had 65-70% homology with published cDNA sequences of Simbu serogroup viruses. The phylogenetic analysis of the data showed that the Oya virus is grouped in Simbu serogroup, but is genetically distinct from the serogroup viruses that have been analyzed molecularly. Serological surveys revealed that the virus distributed widely and densely in Malaysia. Springer 2002 Article PeerReviewed Kono, Y. and Yusnita, Y. and Mohd Ali, A. R. and Maizan, M. and Sharifah, S. H. and Fauzia, O. and Kubo, M. and Aziz, A. J. (2002) Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection. Archives of Virology, 147 (8). pp. 1623-1630. ISSN 0304-8608; eISSN: 1432-8798 https://link.springer.com/article/10.1007/s00705-002-0838-y?error=cookies_not_supported&code=60e8f663-7d1f-4e35-9129-c9fbbee52b6b 10.1007/s00705-002-0838-y |
| spellingShingle | Kono, Y. Yusnita, Y. Mohd Ali, A. R. Maizan, M. Sharifah, S. H. Fauzia, O. Kubo, M. Aziz, A. J. Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection |
| title | Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection |
| title_full | Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection |
| title_fullStr | Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection |
| title_full_unstemmed | Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection |
| title_short | Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection |
| title_sort | characterization and identification of oya virus, a simbu serogroup virus of the genus bunyavirus, isolated from a pig suspected of nipah virus infection |
| url | http://psasir.upm.edu.my/id/eprint/113117/ http://psasir.upm.edu.my/id/eprint/113117/ http://psasir.upm.edu.my/id/eprint/113117/ |