Tropism of subgroup J avian leukosis virus as detected by in situ hybridization
The HPRS-103 strain of avian retrovirus is the prototype of subgroup J avian leukosis virus (ALV-J) and causes myeloid leukosis in meat-type chickens. Using immunohistochemical detection of the viral group-specific antigen (Gag) we have previously demonstrated that the induction of myeloid leukosis...
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| Format: | Article |
| Language: | English |
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Taylor and Francis Group
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113020/ http://psasir.upm.edu.my/id/eprint/113020/1/113020.pdf |
| _version_ | 1848866103607951360 |
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| author | Arshad, S. S. Smith, L. M. Howes, K. Russell, P. H. Venugopal, K. Payne, L. N. |
| author_facet | Arshad, S. S. Smith, L. M. Howes, K. Russell, P. H. Venugopal, K. Payne, L. N. |
| author_sort | Arshad, S. S. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The HPRS-103 strain of avian retrovirus is the prototype of subgroup J avian leukosis virus (ALV-J) and causes myeloid leukosis in meat-type chickens. Using immunohistochemical detection of the viral group-specific antigen (Gag) we have previously demonstrated that the induction of myeloid leukosis by ALV-J is associated with viral tropism for myelomonocytic cells. In this paper we describe an in situ hybridization (ISH) technique using digoxigenin (DIG)-labelled probes for detecting RNA transcripts in tissues from chickens infected with avian leukosis viruses (ALV) of subgroups J (HPRS-103 strain) and A (RAV-1 strain). Virus-specific RNA was detected mainly in the heart, kidney, proventriculus and adrenal in locations similar to those of the Gag protein. Viral gene expression could not be detected in the bone marrow or tumour tissues using this test. Higher levels of viral gene expression in the bursa of Fabricius infected with RAV-1, but not with HPRS-103, might help explain the inability of the latter virus to induce lymphoid leukosis. |
| first_indexed | 2025-11-15T14:15:17Z |
| format | Article |
| id | upm-113020 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:15:17Z |
| publishDate | 1999 |
| publisher | Taylor and Francis Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1130202025-01-22T07:28:31Z http://psasir.upm.edu.my/id/eprint/113020/ Tropism of subgroup J avian leukosis virus as detected by in situ hybridization Arshad, S. S. Smith, L. M. Howes, K. Russell, P. H. Venugopal, K. Payne, L. N. The HPRS-103 strain of avian retrovirus is the prototype of subgroup J avian leukosis virus (ALV-J) and causes myeloid leukosis in meat-type chickens. Using immunohistochemical detection of the viral group-specific antigen (Gag) we have previously demonstrated that the induction of myeloid leukosis by ALV-J is associated with viral tropism for myelomonocytic cells. In this paper we describe an in situ hybridization (ISH) technique using digoxigenin (DIG)-labelled probes for detecting RNA transcripts in tissues from chickens infected with avian leukosis viruses (ALV) of subgroups J (HPRS-103 strain) and A (RAV-1 strain). Virus-specific RNA was detected mainly in the heart, kidney, proventriculus and adrenal in locations similar to those of the Gag protein. Viral gene expression could not be detected in the bone marrow or tumour tissues using this test. Higher levels of viral gene expression in the bursa of Fabricius infected with RAV-1, but not with HPRS-103, might help explain the inability of the latter virus to induce lymphoid leukosis. Taylor and Francis Group 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/113020/1/113020.pdf Arshad, S. S. and Smith, L. M. and Howes, K. and Russell, P. H. and Venugopal, K. and Payne, L. N. (1999) Tropism of subgroup J avian leukosis virus as detected by in situ hybridization. Avian Pathology, 28 (2). pp. 163-169. ISSN 0307-9457; eISSN: 1465-3338 https://www.tandfonline.com/doi/full/10.1080/03079459994894 10.1080/03079459994894 |
| spellingShingle | Arshad, S. S. Smith, L. M. Howes, K. Russell, P. H. Venugopal, K. Payne, L. N. Tropism of subgroup J avian leukosis virus as detected by in situ hybridization |
| title | Tropism of subgroup J avian leukosis virus as detected by in situ hybridization |
| title_full | Tropism of subgroup J avian leukosis virus as detected by in situ hybridization |
| title_fullStr | Tropism of subgroup J avian leukosis virus as detected by in situ hybridization |
| title_full_unstemmed | Tropism of subgroup J avian leukosis virus as detected by in situ hybridization |
| title_short | Tropism of subgroup J avian leukosis virus as detected by in situ hybridization |
| title_sort | tropism of subgroup j avian leukosis virus as detected by in situ hybridization |
| url | http://psasir.upm.edu.my/id/eprint/113020/ http://psasir.upm.edu.my/id/eprint/113020/ http://psasir.upm.edu.my/id/eprint/113020/ http://psasir.upm.edu.my/id/eprint/113020/1/113020.pdf |