Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells
Specific-pathogen-free (SPF) chickens inoculated with low passage Chicken anaemia virus (CAV), SMSC-1 and 3-1 isolates produced lesions suggestive of CAV infection. Repeated passages of the isolates in cell culture until passage 60 (P60) and passage 123 produced viruses that showed a significantly r...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Springer
2003
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/112956/ |
| _version_ | 1848866086798229504 |
|---|---|
| author | Chowdhury, S.M.Z.H. Omar, A.R. Aini, I. Hair-Bejo, M. Jamaluddin, A.A. Md-Zain, B.M. Kono, Y. |
| author_facet | Chowdhury, S.M.Z.H. Omar, A.R. Aini, I. Hair-Bejo, M. Jamaluddin, A.A. Md-Zain, B.M. Kono, Y. |
| author_sort | Chowdhury, S.M.Z.H. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Specific-pathogen-free (SPF) chickens inoculated with low passage Chicken anaemia virus (CAV), SMSC-1 and 3-1 isolates produced lesions suggestive of CAV infection. Repeated passages of the isolates in cell culture until passage 60 (P60) and passage 123 produced viruses that showed a significantly reduced level of pathogenicity in SPF chickens compared to the low passage isolates. Sequence comparison indicated that nucleotide changes in only the coding region of the P60 passage isolates were thought to contribute to virus attenuation. Phylogenetic analysis indicated that SMSC-1 and 3-1 were highly divergent, but their P60 passage derivatives shared significant homology to a Japanese isolate A2. |
| first_indexed | 2025-11-15T14:15:01Z |
| format | Article |
| id | upm-112956 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:15:01Z |
| publishDate | 2003 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1129562025-01-13T02:41:35Z http://psasir.upm.edu.my/id/eprint/112956/ Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells Chowdhury, S.M.Z.H. Omar, A.R. Aini, I. Hair-Bejo, M. Jamaluddin, A.A. Md-Zain, B.M. Kono, Y. Specific-pathogen-free (SPF) chickens inoculated with low passage Chicken anaemia virus (CAV), SMSC-1 and 3-1 isolates produced lesions suggestive of CAV infection. Repeated passages of the isolates in cell culture until passage 60 (P60) and passage 123 produced viruses that showed a significantly reduced level of pathogenicity in SPF chickens compared to the low passage isolates. Sequence comparison indicated that nucleotide changes in only the coding region of the P60 passage isolates were thought to contribute to virus attenuation. Phylogenetic analysis indicated that SMSC-1 and 3-1 were highly divergent, but their P60 passage derivatives shared significant homology to a Japanese isolate A2. Springer 2003 Article PeerReviewed Chowdhury, S.M.Z.H. and Omar, A.R. and Aini, I. and Hair-Bejo, M. and Jamaluddin, A.A. and Md-Zain, B.M. and Kono, Y. (2003) Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells. Archives of Virology, 148 (12). pp. 2437-2448. ISSN 0304-8608; eISSN: 1432-8798 https://link.springer.com/article/10.1007/s00705-003-0189-3?error=cookies_not_supported&code=425fd377-2081-4ec6-b3ea-c13be7b5aecd 10.1007/s00705-003-0189-3 |
| spellingShingle | Chowdhury, S.M.Z.H. Omar, A.R. Aini, I. Hair-Bejo, M. Jamaluddin, A.A. Md-Zain, B.M. Kono, Y. Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells |
| title | Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells |
| title_full | Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells |
| title_fullStr | Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells |
| title_full_unstemmed | Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells |
| title_short | Pathogenicity, sequence and phylogenetic analysis of Malaysian Chicken anaemia virus obtained after low and high passages in MSB-1 cells |
| title_sort | pathogenicity, sequence and phylogenetic analysis of malaysian chicken anaemia virus obtained after low and high passages in msb-1 cells |
| url | http://psasir.upm.edu.my/id/eprint/112956/ http://psasir.upm.edu.my/id/eprint/112956/ http://psasir.upm.edu.my/id/eprint/112956/ |