Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia
Enterococcus species isolated from poultry sources were characterized for their resistance to antibiotics, plasmid content, presence of van genes and their diversity by randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The results showed that all isolates were multi-resistance...
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| Format: | Article |
| Language: | English |
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Elsevier
2001
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| Online Access: | http://psasir.upm.edu.my/id/eprint/112735/ http://psasir.upm.edu.my/id/eprint/112735/1/112735.pdf |
| _version_ | 1848866022436634624 |
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| author | Radu, Son Toosa, Haryanti Abdul Rahim, Raha Reezal, Abdul Ahmad, Maznah Ngah Hamid, Akma Rusul, Gulam Nishibuchi, Mitsuaki |
| author_facet | Radu, Son Toosa, Haryanti Abdul Rahim, Raha Reezal, Abdul Ahmad, Maznah Ngah Hamid, Akma Rusul, Gulam Nishibuchi, Mitsuaki |
| author_sort | Radu, Son |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enterococcus species isolated from poultry sources were characterized for their resistance to antibiotics, plasmid content, presence of van genes and their diversity by randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The results showed that all isolates were multi-resistance to the antibiotics tested. Ampicillin (15/70) followed by chloramphenicol (37/70) were the most active antibiotics tested against the Enterococcus spp. isolates, while the overall resistant rates against the other antibiotics were between 64.3% to 100%. All vancomycin-resistant E. faecalis, E. durans, E. hirae and E. faecium isolates tested by the disk diffusion assay were positive in PCR detection for presence of vanA gene. All E. casseliflavus isolates were positive for vanC2/C3 gene. However, none of the Enterococcus spp. isolates were positive for vanB and vanC1 genes. Plasmids ranging in sizes between 1.1 to ca. 35.8 MDa were detected in 38/70 of the Enterococcus isolates. When the genetic relationship among all isolates of the individual species were tested by RAPD-PCR, genetic differences detected suggested a high genetic polymorphisms of isolates in each individual species. Our results indicates that further epidemiological studies are necessary to elucidate the role of food animals as reservoir of VRE and the public health significance of infections caused by Enterococcus spp. |
| first_indexed | 2025-11-15T14:14:00Z |
| format | Article |
| id | upm-112735 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:14:00Z |
| publishDate | 2001 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1127352025-01-27T08:06:03Z http://psasir.upm.edu.my/id/eprint/112735/ Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia Radu, Son Toosa, Haryanti Abdul Rahim, Raha Reezal, Abdul Ahmad, Maznah Ngah Hamid, Akma Rusul, Gulam Nishibuchi, Mitsuaki Enterococcus species isolated from poultry sources were characterized for their resistance to antibiotics, plasmid content, presence of van genes and their diversity by randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The results showed that all isolates were multi-resistance to the antibiotics tested. Ampicillin (15/70) followed by chloramphenicol (37/70) were the most active antibiotics tested against the Enterococcus spp. isolates, while the overall resistant rates against the other antibiotics were between 64.3% to 100%. All vancomycin-resistant E. faecalis, E. durans, E. hirae and E. faecium isolates tested by the disk diffusion assay were positive in PCR detection for presence of vanA gene. All E. casseliflavus isolates were positive for vanC2/C3 gene. However, none of the Enterococcus spp. isolates were positive for vanB and vanC1 genes. Plasmids ranging in sizes between 1.1 to ca. 35.8 MDa were detected in 38/70 of the Enterococcus isolates. When the genetic relationship among all isolates of the individual species were tested by RAPD-PCR, genetic differences detected suggested a high genetic polymorphisms of isolates in each individual species. Our results indicates that further epidemiological studies are necessary to elucidate the role of food animals as reservoir of VRE and the public health significance of infections caused by Enterococcus spp. Elsevier 2001 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112735/1/112735.pdf Radu, Son and Toosa, Haryanti and Abdul Rahim, Raha and Reezal, Abdul and Ahmad, Maznah and Ngah Hamid, Akma and Rusul, Gulam and Nishibuchi, Mitsuaki (2001) Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia. Diagnostic Microbiology and Infectious Disease, 39 (3). pp. 145-153. ISSN 0732-8893; eISSN: 1879-0070 https://linkinghub.elsevier.com/retrieve/pii/S073288930100222X 10.1016/s0732-8893(01)00222-x |
| spellingShingle | Radu, Son Toosa, Haryanti Abdul Rahim, Raha Reezal, Abdul Ahmad, Maznah Ngah Hamid, Akma Rusul, Gulam Nishibuchi, Mitsuaki Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia |
| title | Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia |
| title_full | Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia |
| title_fullStr | Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia |
| title_full_unstemmed | Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia |
| title_short | Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia |
| title_sort | occurrence of the vana and vanc2/c3 genes in enterococcus species isolated from poultry sources in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/112735/ http://psasir.upm.edu.my/id/eprint/112735/ http://psasir.upm.edu.my/id/eprint/112735/ http://psasir.upm.edu.my/id/eprint/112735/1/112735.pdf |