Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria
Enterobacterial repetitive intergenic consensus based on PCR (ERIC-PCR) was used to generate DNA fingerprints for bifidobacteria and other probiotic bacteria. Two primers (ERIC 1R and ERIC 2) used in ERIC-PCR revealed that all of the probiotic bacteria tested possess enterobacterial repetitive inter...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer
2001
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| Online Access: | http://psasir.upm.edu.my/id/eprint/112670/ http://psasir.upm.edu.my/id/eprint/112670/1/112670.pdf |
| _version_ | 1848866005816705024 |
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| author | Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. |
| author_facet | Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. |
| author_sort | Shuhaimi, M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enterobacterial repetitive intergenic consensus based on PCR (ERIC-PCR) was used to generate DNA fingerprints for bifidobacteria and other probiotic bacteria. Two primers (ERIC 1R and ERIC 2) used in ERIC-PCR revealed that all of the probiotic bacteria tested possess enterobacterial repetitive intergenic consensus sequences with the PCR products ranging from 250 bp to 5000 bp. The bacterial strains can be differentiated by comparing fingerprint patterns. The dendrogram of the fingerprints revealed that most of the bifidobacterial wild type strains fell into one cluster at similarity level of approximately 79%. |
| first_indexed | 2025-11-15T14:13:44Z |
| format | Article |
| id | upm-112670 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:13:44Z |
| publishDate | 2001 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1126702025-03-06T00:58:00Z http://psasir.upm.edu.my/id/eprint/112670/ Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. Enterobacterial repetitive intergenic consensus based on PCR (ERIC-PCR) was used to generate DNA fingerprints for bifidobacteria and other probiotic bacteria. Two primers (ERIC 1R and ERIC 2) used in ERIC-PCR revealed that all of the probiotic bacteria tested possess enterobacterial repetitive intergenic consensus sequences with the PCR products ranging from 250 bp to 5000 bp. The bacterial strains can be differentiated by comparing fingerprint patterns. The dendrogram of the fingerprints revealed that most of the bifidobacterial wild type strains fell into one cluster at similarity level of approximately 79%. Springer 2001 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112670/1/112670.pdf Shuhaimi, M. and Ali, A.M. and Saleh, N.M. and Yazid, A.M. (2001) Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria. Biotechnology Letters, 23 (9). pp. 731-736. ISSN 0141-5492; eISSN: 1573-6776 https://link.springer.com/article/10.1023/A:1010355003674?error=cookies_not_supported&code=1f499e90-de83-4e55-a364-fcd84f02fa62 10.1023/a:1010355003674 |
| spellingShingle | Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria |
| title | Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria |
| title_full | Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria |
| title_fullStr | Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria |
| title_full_unstemmed | Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria |
| title_short | Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria |
| title_sort | utilisation of enterobacterial repetitive intergenic consensus (eric) sequence-based pcr to fingerprint the genomes of bifidobacterium isolates and other probiotic bacteria |
| url | http://psasir.upm.edu.my/id/eprint/112670/ http://psasir.upm.edu.my/id/eprint/112670/ http://psasir.upm.edu.my/id/eprint/112670/ http://psasir.upm.edu.my/id/eprint/112670/1/112670.pdf |