Optimization of osmotic shock process variables for enhancement of the release of periplasmic interferon-α2b from Escherichia coli using response surface method

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Optimization of osmotic shock process variables for enhancement of the release of periplasmic interferon-α2b from Escherichia coli using response surface method

The osmotic shock process for the release of periplasmic recombinant human interferon-α2b from Escherichia coli was optimized using response surface method (RSM). The process parameters such as pH, buffer concentration and sucrose concentration in hypertonic solution, cell concentration to hypertoni...

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Bibliographic Details
Main Authors:	Ramanana, Ramakrishnan Nagasundara, Tana, Joo Shun, Mohamed, Mohd Shamzi, Tau, Chuan Ling, Tey, Beng Ti, Ariff, Arbakariya
Format:	Article
Language:	English English
Published:	Elsevier 2010
Online Access:	http://psasir.upm.edu.my/id/eprint/11265/ http://psasir.upm.edu.my/id/eprint/11265/1/Optimization%20of%20osmotic%20shock%20process%20variables%20for%20enhancement%20of%20the%20release%20of%20periplasmic%20interferon.pdf

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